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Team:Groningen/Notebook/Wetwork 11July2012
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| - | Plasmid isolation of Biobrick parts BBa_K116603, BBa_K116602, BBa_J31019, BBa_K116639, BBa_K116609. Concentrations of 69.9, 76.5, 109.8, 70.2, 106.2 ng/ul respectively. | + | <body><br><br><br> |
| - | EcoRI restiction of with these isolated plasmids. | + | <p class="margin"> |
| - | + | Tom/Emeraldo<br> | |
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| - | Arjan/Renske | + | AlsT PCR with Pfu and Taq polymerase at 60 °C with primers 150, 250, 300, 500 bp.<br> |
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| - | D-Day: washed and scanned the slides! The slides were subsequently washed using the ArrayIt® Microarray Wash Station and dried by centrifuging (2 min, 2000 rpm, RT). Slides were scanned using a Genepix Professional 4200AL (Axon instruments, Union City CA USA) array scanner, using the Genepix Pro 6.1 software (Axon instruments), default settings for Cy3/Cy5. | + | Nisa/Emeraldo<br> |
| - | + | <br> | |
| - | < | + | Plasmid isolation of Biobrick parts BBa_K116603, BBa_K116602, BBa_J31019, BBa_K116639, BBa_K116609. Concentrations of 69.9, 76.5, 109.8, 70.2, 106.2 ng/ul respectively.<br> |
| + | EcoRI restiction of with these isolated plasmids.<br> | ||
| + | <br> | ||
| + | <br> | ||
| + | Arjan/Renske<br> | ||
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| + | D-Day: washed and scanned the slides! The slides were subsequently washed using the ArrayIt® Microarray Wash Station and dried by centrifuging (2 min, 2000 rpm, RT). Slides were scanned using a Genepix Professional 4200AL (Axon instruments, Union City CA USA) array scanner, using the Genepix Pro 6.1 software (Axon instruments), default settings for Cy3/Cy5.<br> | ||
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<A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A> | <A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A> | ||
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Latest revision as of 21:03, 25 September 2012
Tom/Emeraldo
AlsT PCR with Pfu and Taq polymerase at 60 °C with primers 150, 250, 300, 500 bp.
Nisa/Emeraldo
Plasmid isolation of Biobrick parts BBa_K116603, BBa_K116602, BBa_J31019, BBa_K116639, BBa_K116609. Concentrations of 69.9, 76.5, 109.8, 70.2, 106.2 ng/ul respectively.
EcoRI restiction of with these isolated plasmids.
Arjan/Renske
D-Day: washed and scanned the slides! The slides were subsequently washed using the ArrayIt® Microarray Wash Station and dried by centrifuging (2 min, 2000 rpm, RT). Slides were scanned using a Genepix Professional 4200AL (Axon instruments, Union City CA USA) array scanner, using the Genepix Pro 6.1 software (Axon instruments), default settings for Cy3/Cy5.
Back to notebook
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