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Team:Grenoble/Biology/Protocols/Gel
From 2012.igem.org
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<ol> | <ol> | ||
<li>When the gel is solid, remove carefully the comb.</li> | <li>When the gel is solid, remove carefully the comb.</li> | ||
| - | <li>Place the gel in the buffer tank and cover it with the buffer solution.</li> | + | <li>Place the gel in the buffer tank and cover it with the 1X TAE buffer solution.</li> |
<li>Load each sample into the wells (don't forget the loading dye and the DNA ladder).</li> | <li>Load each sample into the wells (don't forget the loading dye and the DNA ladder).</li> | ||
</ol> | </ol> | ||
Revision as of 08:58, 28 August 2012
Gel electrophoresis
Goal
Separate DNA strands of different lengths.Prepare the gel
- Add 1 g of agarose powder to 50 mL of 1X TAE buffer.
- Heat the solution using the microwave until the powder is completely dissolved.
- Once the solution is cool enough to be touched, pour it into gel cast (don't forget the comb).
Load the gel
- When the gel is solid, remove carefully the comb.
- Place the gel in the buffer tank and cover it with the 1X TAE buffer solution.
- Load each sample into the wells (don't forget the loading dye and the DNA ladder).
