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Team:Grenoble/Biology/Protocols/Gel
From 2012.igem.org
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<h2>Prepare the gel</h2> | <h2>Prepare the gel</h2> | ||
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<li>Add 1 g of agarose powder to 50 mL of 1X TAE buffer.</li> | <li>Add 1 g of agarose powder to 50 mL of 1X TAE buffer.</li> | ||
<li>Heat the solution using the microwave until the powder is completely dissolved.</li> | <li>Heat the solution using the microwave until the powder is completely dissolved.</li> | ||
<li>Once the solution is cool enough to be touched, pour it into gel cast (don't forget to pull the comb).</li> | <li>Once the solution is cool enough to be touched, pour it into gel cast (don't forget to pull the comb).</li> | ||
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</section> | </section> | ||
</div> | </div> | ||
Revision as of 20:09, 27 August 2012
Gel electrophoresis
Goal
Separate DNA strands of different lengths.Prepare the gel
- Add 1 g of agarose powder to 50 mL of 1X TAE buffer.
- Heat the solution using the microwave until the powder is completely dissolved.
- Once the solution is cool enough to be touched, pour it into gel cast (don't forget to pull the comb).
