Revision as of 07:59, 23 August 2012

iGEM Grenoble 2012

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LB Agar plates preparation

Goal

Prepare solid medium to culture bacteria.

Prepare the LB Agar

In a 500mL bottle add 6.4g of bacteriological agar to 400mL of LB medium. Then sterilize it using the autoclave.
You can store it at room temperature, but before use it, you need to heat it up.

Add the right antibiotic

If you want to prepare LB Agar plates with antibiotics you need to:

heat the LB Agar up, in order to melt it down
after the melting, wait for the solution to cool down
when the solution is not so hot (around 50°C), add the antibiotic solution (in a flow hood of course)

Antibiotics concentrations:

Antibiotics	Finale concentration
Chloramphenicol
Kanamycin
Ampicillin

Pour into plates

When the antibiotic solution is added or when the bottle is not so hot (around 50°C), you can pour into plates:

In a flow hood, pour approximately 15mL of the LB Agar solution into each plate
Let each plate cool until it becomes solid
When it's ok, flip the plates (bottom-up), write on the back and store them at 4°C

@@ Line 47: / Line 47: @@
 </section>
 <section>
-     <h2>Pour into petri dishes</h2>
+     <h2>Pour into plates</h2>
      When the antibiotic solution is added or when the bottle is not so hot (around 50°C), you can pour into plates:
      <ul>
          <li>In a flow hood, pour approximately 15mL of the LB Agar solution into each plate</li>
          <li>Let each plate cool until it becomes solid</li>
-         <li>When it's ok, flip the plate(bottom-up), write on the back and store them at 4°C</li>
+         <li>When it's ok, flip the plates (bottom-up), write on the back and store them at 4°C</li>
      </ul>

Team:Grenoble/Biology/Protocols/LB

From 2012.igem.org