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Team:Grenoble/Biology/Protocols/LB
From 2012.igem.org
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<li>heat the LB Agar up, in order to solubilize it</li> | <li>heat the LB Agar up, in order to solubilize it</li> | ||
<li>after the solubilization, wait for the solution to refresh</li> | <li>after the solubilization, wait for the solution to refresh</li> | ||
| - | <li>when the solution is not so hot, you can add the antibiotic solution</li> | + | <li>when the solution is not so hot, you can add the antibiotic solution (in a flow hood of course)</li> |
</ul> | </ul> | ||
<br/> | <br/> | ||
Revision as of 07:36, 23 August 2012
LB Agar plates preparation
Goal
Prepare solid medium to culture bacteria.Prepare the LB Agar
In a 500mL bottle add 6.4g of bacteriological agar to 400mL of LB medium. Then sterelize it using the autoclave.You can store it at room temperature, but before use it, you need to heat it up.
Add the right antibiotic
If you want to prepare LB Agar plates added with antibiotics you need to:- heat the LB Agar up, in order to solubilize it
- after the solubilization, wait for the solution to refresh
- when the solution is not so hot, you can add the antibiotic solution (in a flow hood of course)
Antibiotics concentrations:
| Antibiotics | Finale concentration |
|---|---|
| Chloramphenicol | |
| Kanamycin | |
| Ampicillin |
