Team:Grenoble/Biology/Protocols/LB

From 2012.igem.org

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     <h2>Add the antibiotic</h2>
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     <h2>Add the right antibiotic</h2>
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     If you want to prepare LB Agar plates added with antibiotics you need to:
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    <ul>
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        <li>heat the LB Agar up, in order to solubilize it</li>
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        <li>after the solubilization, wait for the solution to refresh</li>
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        <li>when the solution is not so hot, you can add the antibiotic solution</li>
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    </ul>
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    <br/>
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    <h3>Antibiotics concentrations</h3>
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    <br/>
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    <table>
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        <tr>
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            <th>Antibiotics</th>
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            <th>Finale concentration</th>
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        <tr>
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            <td>Chloramphenicol</td>
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            <td></td>
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        </tr>
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        <tr>
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            <td>Kanamycin</td>
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            <td></td>
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        </tr>
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        <tr>
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            <td>Ampicillin</td>
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            <td></td>
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        </tr>
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    </table>
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Revision as of 07:31, 23 August 2012

iGEM Grenoble 2012

iGEM Grenoble iGEM
iGEM 2012
main page
Project

LB Agar plates preparation

Goal

Prepare solid medium to culture bacteria.

Prepare the LB Agar

In a 500mL bottle add 6.4g of bacteriological agar to 400mL of LB medium.
Then sterelize using the autoclave.
You can store it at room temperature, but before use it, you need to heat it up.

Add the right antibiotic

If you want to prepare LB Agar plates added with antibiotics you need to:
  • heat the LB Agar up, in order to solubilize it
  • after the solubilization, wait for the solution to refresh
  • when the solution is not so hot, you can add the antibiotic solution

Antibiotics concentrations


Antibiotics Finale concentration
Chloramphenicol
Kanamycin
Ampicillin

Pour the plates

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