Team:Grenoble/Biology/Notebook/June/week 25

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<a href="https://2012.igem.org/Team:Grenoble/Biology/Notebook/June/week_25">Week 25</a> •  
<a href="https://2012.igem.org/Team:Grenoble/Biology/Notebook/June/week_25">Week 25</a> •  
<a href="https://2012.igem.org/Team:Grenoble/Biology/Notebook/June/week_26">Week 26</a>
<a href="https://2012.igem.org/Team:Grenoble/Biology/Notebook/June/week_26">Week 26</a>
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<h1> Week 23: June 04<span class="exposant">th</span> to 10<span class="exposant">th</span> </h1>
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<h1> Week 25: June 18<span class="exposant">th</span> to 24<span class="exposant">th</span> </h1>
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During this week we chose the project on which we wanted to work. <br/>
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After a meeting with our instructors, we decided to work on a second amplifier (cAMP-CRP system). We designed the genetic network for the second amplifier according to results we found in the literature: <br/>
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We decided to create a system which can detect a pathogene, like <i>Staphylococcus aureus</i>, for example.<br/>
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<center><img src="https://static.igem.org/mediawiki/2012/d/d5/Network_cAMP-CRP.jpg" alt="pLAC_rsmY"/></center>
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We intend to build an ultra sensitive detector. The goal is to produce a detector which can greatly amplify an extremely low input signal. It consists of three modules:<br/>
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We looked for the biobricks involved in this network and we designed the primers for both amplifiers (RsmA-rsmY and cAMP-CRP systems) that we wanted to test in parallel.<br/>
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    <li>the first gets the signal</li>
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    <li>the second amplifies it</li>
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    <li>the third sends the output</li>
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The detection module is a membrane receptor that once activated by a biomolecule actuates an amplification loop. The loop enhances the cell response efficiency. <br/>
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We looked for the plasmids on which we wanted to insert the selected biobricks in order to construct the amplifiers and to test them. <br/>
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The amplification system contains a genetic feed forward loop. It acts as a biologic loud filter aiming at reducing false positive outputs. Once amplified and filtered the signal is transmitted to the neighboring bacteria. In this way a noticeable fluorescent emission is generated. <br/>
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We searched in the literature to find a way to build a detection module.<br/>
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<center> <h4>Scheme of the concept</h4> </center>
 
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<center><img src="https://static.igem.org/mediawiki/2012/9/9d/Schema1.png" alt="Scheme of the concept" style="horizontal-align: center;"/></center>
 
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<center> <h4>Theoretical response of the device depending on the input signal</h4> <br/>
 
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<img src="https://static.igem.org/mediawiki/2012/c/cf/Schema2.png" alt="Theoretical response of the device depending on the input signal" style="horizontal-align: center;"/></center>
 
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Revision as of 12:27, 20 August 2012

iGEM Grenoble 2012

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June

Week 23 • Week 24 • Week 25 • Week 26


Week 25: June 18th to 24th

After a meeting with our instructors, we decided to work on a second amplifier (cAMP-CRP system). We designed the genetic network for the second amplifier according to results we found in the literature:

pLAC_rsmY

We looked for the biobricks involved in this network and we designed the primers for both amplifiers (RsmA-rsmY and cAMP-CRP systems) that we wanted to test in parallel.

We looked for the plasmids on which we wanted to insert the selected biobricks in order to construct the amplifiers and to test them.

We searched in the literature to find a way to build a detection module.



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