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Team:Grenoble/Biology/Notebook/June/week 26
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<center><img src="https://static.igem.org/mediawiki/2012/d/d5/Network_cAMP-CRP.jpg" alt="pLAC_rsmY"/></center> | <center><img src="https://static.igem.org/mediawiki/2012/d/d5/Network_cAMP-CRP.jpg" alt="pLAC_rsmY"/></center> | ||
| + | <h3>Amplifier 2: cAMP-CRP system (first construction): (1 final plasmid)</h3> | ||
| + | <h4>Plasmid Mapping</h4> | ||
| + | On the pSB4K5 plasmid, we wanted to put the construction: pAra/Bad_RBS_GFP_RBS_Cya | ||
| + | <center><img src="" alt="pLAC_fha1_eCFP"/></center> | ||
| + | <h4>Biobricks involved</h4> | ||
| + | pSB4K5 plasmid => final length ≃ 2400bp | ||
| + | <h4>New parts</h4> | ||
| + | pAra/Bad_RBS_GFP (Grenoble lab LAPM) => final length ≃ 1300bp <br/> | ||
| + | RBS_Cya (E. coli chromosom) => final length ≃ 2600bp<br/> | ||
</section> | </section> | ||
Revision as of 12:45, 6 August 2012
Week 26: June 25th to July 01st
For the detection module, we decided to work on a modified membrane receptor. It consists of two merged E. coli membrane receptors. The extracellular part is the extracellular part of Tap, a dipeptide membrane receptor. The cytoplasmic part is the cytoplasmic part of the EnvZ receptor, which has the ability to activate OmpR (by phosphorilation) which is a transcription factor.
It will be a proof of concept for a bigger system which is a complete pathogene detection module :
1st network : with amplifier 1 (RsmA-rsmY)
Amplifier 1 : RsmA-rsmY system characterisation: (3 final plasmids)
Plasmid Mapping
On a pSB4K5 plasmid, we wanted to put the construction: pLAC_fha1_eCFP
Biobricks involved
pLAC_RBS (BBa_I13601) => final length ≃ 90bppLAC (BBa_I13601) => final length ≃ 90bp
eCFP (BBa_E0422 or BBa_E0022) => final length ≃ 800bp
plasmid pSB4K5 => final length ≃ 2400bp
plasmid pSB3C5 => final length ≃ 2400bp
plasmid pSB1A3 => final length ≃ 2400bp
New parts
RsmA (iGEM Grenoble 2011) => final length ≃ 200bprsmY (iGEM Grenoble 2011) => final length ≃ 170bp
fha1 (iGEM Grenoble 2011) => final length ≃ 80bp
2nd network : with amplifier 2 (cAMP-CRP)
Amplifier 2: cAMP-CRP system (first construction): (1 final plasmid)
Plasmid Mapping
On the pSB4K5 plasmid, we wanted to put the construction: pAra/Bad_RBS_GFP_RBS_CyaBiobricks involved
pSB4K5 plasmid => final length ≃ 2400bpNew parts
pAra/Bad_RBS_GFP (Grenoble lab LAPM) => final length ≃ 1300bpRBS_Cya (E. coli chromosom) => final length ≃ 2600bp
