"
Team:Grenoble/Biology/Notebook/June/week 26
From 2012.igem.org
(Difference between revisions)
| Line 17: | Line 17: | ||
</p> | </p> | ||
| - | <p | + | <p> |
<br/> | <br/> | ||
It will be a proof of concept for a bigger system which is a complete pathogene detection module : | It will be a proof of concept for a bigger system which is a complete pathogene detection module : | ||
| Line 26: | Line 26: | ||
<center><img src="https://static.igem.org/mediawiki/2012/3/33/Network_RsmA-rsmY.jpg" alt="amplifier_1"/></center> | <center><img src="https://static.igem.org/mediawiki/2012/3/33/Network_RsmA-rsmY.jpg" alt="amplifier_1"/></center> | ||
| + | |||
| + | <h3>Amplifier 1 : RsmA-rsmY system characterisation: (3 final plasmids)</h3> | ||
| + | <h4>Plasmid Mapping</h4> | ||
| + | On a pSB4K5 plasmid, we wanted to put the construction: pLAC_fha1_eCFP | ||
| + | <center><img src="https://static.igem.org/mediawiki/2012/3/33/Network_RsmA-rsmY.jpg" alt="amplifier_1"/></center> | ||
| + | On a pSB3C5 plasmid, we wanted to put the construction: pLAC_RBS_RsmA | ||
| + | On a pSB1A3 plasmid, we wanted to put the construction: pLAC_rsmY | ||
| + | <center><img src="https://static.igem.org/mediawiki/2012/3/33/Network_RsmA-rsmY.jpg" alt="amplifier_1"/></center> | ||
| + | |||
| + | <h4>Biobricks involved</h4> | ||
| + | pLAC_RBS (BBa_I13601) => final length ≃ 90bp | ||
| + | pLAC (BBa_I13601) => final length ≃ 90bp | ||
| + | eCFP (BBa_E0422 or BBa_E0022) => final length ≃ 800bp | ||
| + | plasmid pSB4K5 => final length ≃ 2400bp | ||
| + | plasmid pSB3C5 => final length ≃ 2400bp | ||
| + | plasmid pSB1A3 => final length ≃ 2400bp | ||
| + | <h4>New parts</h4> | ||
| + | RsmA (iGEM Grenoble 2011) => final length ≃ 200bp | ||
| + | rsmY (iGEM Grenoble 2011) => final length ≃ 170bp | ||
| + | fha1 (iGEM Grenoble 2011) => final length ≃ 80bp | ||
| + | |||
</section> | </section> | ||
Revision as of 10:06, 6 August 2012
Week 26: June 25th to July 01st
For the detection module, we decided to work on a modified membrane receptor. It consists of two merged E. coli membrane receptors. The extracellular part is the extracellular part of Tap, a dipeptide membrane receptor. The cytoplasmic part is the cytoplasmic part of the EnvZ receptor, which has the ability to activate OmpR (by phosphorilation) which is a transcription factor.
It will be a proof of concept for a bigger system which is a complete pathogene detection module :
1st network : with amplifier 1 (RsmA-rsmY)
Amplifier 1 : RsmA-rsmY system characterisation: (3 final plasmids)
Plasmid Mapping
On a pSB4K5 plasmid, we wanted to put the construction: pLAC_fha1_eCFP
