Latest revision as of 17:31, 18 November 2012

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Post-Jamboree Accomplishments

Our team has accomplished so much this year!

Gold Medallist
Best Human Practices, Americas West
Best Wiki, Americas West
Best Poster, Americas West
Best Model, Americas West
Safety Commendation, Americas West
Best Human Practices, World Championship
"Sweet Sixteen", World Championship

See all of the iGEM 2012 results here and here!

See what we accomplished since the Americas West Regionals!

In our Human Practices project, we...

Returned to industry experts to see if we accomplished our goals and what the next steps should be.

Characterized the functionality of our previously submitted Prha (BBa_K902066) promoter using GFP fluorescence.

Tested an additional complete kill switch device using the Prha (BBa_K902084) promoter with our S7 kill gene.

Tested a glycine auxotroph killswitch.

In terms of FRED, we...

Electrochemically characterized an inducible lacz (BBa_K902090) circuit, replacing a faulty existing circuit in the registry containing a frameshift.
Tested one of our transposon library hits with our electrochemical reporter, showing that we can use our system to detect toxins electrochemically.
Validated our system with tailings using our transposon system and electrochemical reporter to be able to selectively detect toxins on real oil sands samples.

In terms of OSCAR, we...

Obtained additional characterization data in validation of our flux-variability analysis model.
Characterized the ability of our novel amdA (BBa_K902041) BioBrick to selectively remove primary amides from ring structures with remarkable efficiency, turning them into a substrate that the Petrobrick (BBa_K590025) can likely convert into hydrocarbons.
Validated a scale up model for using our bioreactor/belt skimmer system in producing and extracting hydrocarbons.

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-TITLE=Post-Regional Achievements|CONTENT=
+{{Team:Calgary/BasicPage|proj_hp|
+SECTION = Project|
+SIDELIST =
 <html>
-<p>Here is a collection of all our achievements in the past two weeks.</p>
+<head>
+<style>
+/*link coloring schema*/
+a.green{
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+a.blue{
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+#sidebar #list{
+	border-left: 10px solid #C6D !important;
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+</style>
+</head>
+<ul>
+	<li>
+		<a class="drop" href="https://2012.igem.org/Team:Calgary/Project">Overview</a>
+		<ul>
+			<li><a href="https://2012.igem.org/Team:Calgary/Project/DataPage">Data Page</a></li>
+<li><a href="https://2012.igem.org/Team:Calgary/Project/Accomplish">Accomplishments</a></li>
+<li><a href="https://2012.igem.org/Team:Calgary/Project/Post-Regionals">Post-Jamboree</a></li>
+		</ul>
+	</li>
+	<li>
+		<a class="drop" href="https://2012.igem.org/Team:Calgary/Project/HumanPractices">Human Practices</a>
+		<ul>
+			<li><a href="https://2012.igem.org/Team:Calgary/Project/HumanPractices/Collaborations">Initiative</a></li>
+			<li><a href="https://2012.igem.org/Team:Calgary/Project/HumanPractices/Interviews">Interviews</a></li>
+			<li><a href="https://2012.igem.org/Team:Calgary/Project/HumanPractices/Design">Design</a></li>
+			<li><a href="https://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch">Killswitch</a></li><ul><li><a href="https://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch/Regulation">Regulation</a></li>
+<li><a href="https://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch/KillGenes">Kill Genes</a></li></ul>
+			<li><a href="https://2012.igem.org/Team:Calgary/Safety">Safety</a></li>
+		</ul>
+	</li>
+	<li>
+		<a class="drop" href="https://2012.igem.org/Team:Calgary/Project/FRED">FRED</a>
+		<ul>
+			<li><a href="https://2012.igem.org/Team:Calgary/Project/FRED/Detecting">Toxin Sensing</a></li>
+			<li><a href="https://2012.igem.org/Team:Calgary/Project/FRED/Reporting">Electroreporting</a></li>
+			<li><a href="https://2012.igem.org/Team:Calgary/Project/FRED/Modelling">Modelling</a></li>
+			<li><a href="https://2012.igem.org/Team:Calgary/Project/FRED/Prototype">Device Prototype</a></li>
+		</ul>
+	</li>
+	<li>
+		<a class="drop" href="https://2012.igem.org/Team:Calgary/Project/OSCAR">OSCAR</a>
+		<ul>
+			<li><a href="https://2012.igem.org/Team:Calgary/Project/OSCAR/Decarboxylation">Decarboxylation</a></li>
+<li><a href="https://2012.igem.org/Team:Calgary/Project/OSCAR/CatecholDegradation">Decatecholization</a></li>
+<li><a href="https://2012.igem.org/Team:Calgary/Project/OSCAR/FluxAnalysis">Flux Analysis</a></li>
+<li><a href="https://2012.igem.org/Team:Calgary/Project/OSCAR/Bioreactor">Bioreactor</a></li>
+<li><a href="https://2012.igem.org/Team:Calgary/Project/OSCAR/Upgrading">Upgrading</a></li><ul>
+			<li><a href="https://2012.igem.org/Team:Calgary/Project/OSCAR/Desulfurization">Desulfurization</a></li>
+			<li><a href="https://2012.igem.org/Team:Calgary/Project/OSCAR/Denitrogenation">Denitrogenation</a></li></ul>
+		</ul>
+<li><a href="https://2012.igem.org/Team:Calgary/Project/Synergy">Synergy</a></li>
+	</li>
+<li><a href="https://2012.igem.org/Team:Calgary/Project/References">References</a></li>
+	<li><a href="https://2012.igem.org/Team:Calgary/Project/Attributions">Attributions</a></li>
+</ul>
+</html>|
+TITLE=Post-Jamboree Accomplishments|CONTENT=
+<html>
+<img src="https://static.igem.org/mediawiki/2012/3/3d/UCalgary2012_FRED_and_OSCAR_Achievements.png" style="width: 280px; float: right; padding: 10px;"></img>
+<h2>Our team has accomplished so much this year!</h2>
+<ul>
+<li><p>Gold Medallist</p></li>
+<li><p>Best Human Practices, Americas West</p></li>
+<li><p>Best Wiki, Americas West</p></li>
+<li><p>Best Poster, Americas West</p></li>
+<li><p>Best Model, Americas West</p></li>
+<li><p>Safety Commendation, Americas West</p></li>
+<li><p>Best Human Practices, World Championship</p></li>
+<li><p>"Sweet Sixteen", World Championship</p></li>
+</ul>
+<p>See all of the iGEM 2012 results <a href="https://2012.igem.org/Jamborees" target="_blank">here</a> and <a href="https://igem.org/Results?year=2012&region=Championship&division=igem" target="_blank">here</a>!</p>
+<h2>See what we accomplished since the Americas West Regionals!</h2>
+<p><b>In our <FONT COLOR="FF7A00">Human Practices</FONT> project, we...</b></p>
+<ul>
+<li><p><a class="orange" href="https://2012.igem.org/Team:Calgary/Project/HumanPractices/Interviews"><b>Returned to industry experts</b></a> to see if we accomplished our goals and what the <a class="orange" href="https://2012.igem.org/Team:Calgary/Project/Synergy"><b>next steps</b></a> should be.</li></p>
+<li><p><a class="orange" href="https://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch/Regulation#Prha_results"><b>Characterized</b></a> the functionality of our previously submitted <a class="orange" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K902066"><b><i>Prha</i> (BBa_K902066)</b></a> promoter using GFP fluorescence.</li></p>
+<li><p>Tested an additional <a class="orange" href="https://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch/Regulation"><b>complete kill switch device</b></a> using the <a class="orange" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K902084"><b><i>Prha</i> (BBa_K902084)</b></a> promoter with our S7 kill gene.</li></p>
+<li><p>Tested a <a class="orange" href="https://2012.igem.org/Team:Calgary/Project/Synergy"><b>glycine auxotroph killswitch</b></a>.</li></p></ul>
+<br>
+<p><b>In terms of <FONT COLOR=#159900>FRED</FONT>, we...</b></p>
+<ul>
+<li><p><a class="green" href="https://2012.igem.org/Team:Calgary/Project/FRED/Reporting"><b>Electrochemically characterized</b></a> an inducible <a class="green" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K902090"><b><i>lacz</i> (BBa_K902090)</b></a> circuit, replacing a faulty existing circuit in the registry containing a frameshift.</p></li>
+<li><p><a class="green" href="https://2012.igem.org/Team:Calgary/Project/Synergy"><b>Tested one of our transposon library hits</b></a> with our electrochemical reporter, showing that we can use our system to <b>detect toxins electrochemically</b>.</p></li>
+<li><p><a class="green" href="https://2012.igem.org/Team:Calgary/Project/Synergy"><b>Validated our system with tailings</b></a> using our transposon system and electrochemical reporter to be able to selectively detect toxins on <b>real oil sands samples</b>.</p></li>
+</ul>
+<br>
+<p><b>In terms of <FONT COLOR=#1088CC>OSCAR</FONT>, we...</b></p>
+<ul>
+<li><p>Obtained <a class="blue" href="https://2012.igem.org/Team:Calgary/Project/OSCAR/FluxAnalysis"><b>additional characterization data</b></a> in validation of our flux-variability analysis model.</p></li>
+<li><p>Characterized the ability of our <b>novel</b> <a class="blue" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K902041"><b><i>amdA</i> (BBa_K902041)</b></a> <b>BioBrick</b> to selectively remove primary amides from ring structures with remarkable efficiency, turning them into a substrate that the <a class="blue" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K590025"><b>Petrobrick (BBa_K590025)</b></a> can likely convert into hydrocarbons.</p></li>
+<li><p><a class="blue" href="https://2012.igem.org/Team:Calgary/Project/Synergy"><b>Validated a scale up model</b></a> for using our bioreactor/belt skimmer system in producing and extracting hydrocarbons.</p></li>
+</ul>
+<h2>
+<ul>
+</p>
 </html>
 }}