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Team:Calgary/Project/Post-Regionals
From 2012.igem.org
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<li><p>Tested an additional <a class="orange" href="https://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch/Regulation"><b>complete kill switch device</b></a> using the <a class="orange" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K902084"><b><i>Prha</i> (BBa_K902084)</b></a> promoter with our S7 kill gene.</li></p> | <li><p>Tested an additional <a class="orange" href="https://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch/Regulation"><b>complete kill switch device</b></a> using the <a class="orange" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K902084"><b><i>Prha</i> (BBa_K902084)</b></a> promoter with our S7 kill gene.</li></p> | ||
| - | <li><p><a class="orange" href="https://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch/Regulation"><b>Further characterized</b></a> our previously validated <b>magnesium riboswitch kill gene construct</b><a class="orange" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K902018"> <b>(BBa_K902018)</b></a>.</li></p></ul> | + | <li><p><a class="orange" href="https://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch/Regulation"><b>Further characterized</b></a> our previously validated <b>magnesium riboswitch kill gene construct</b><a class="orange" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K902018"> <b>(BBa_K902018)</b></a>.</li></p> |
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| + | <li><p>Tested a <a class="purple" href="https://2012.igem.org/Team:Calgary/Project/Synergy">glycine auxotroph killswitch</a>.</li></p></ul> | ||
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Revision as of 03:56, 27 October 2012
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Post-Regional Accomplishments
Our team has had many accomplishments since the regional jamboree!
In our Human Practices project, we...
Returned to industry experts to see if we accomplished our goals and what the next steps should be.
Characterized the functionality of our previously submitted Prha (BBa_K902066) promoter using GFP fluorescence.
Tested an additional complete kill switch device using the Prha (BBa_K902084) promoter with our S7 kill gene.
Further characterized our previously validated magnesium riboswitch kill gene construct (BBa_K902018).
Tested a glycine auxotroph killswitch.
In terms of FRED, we...
Electrochemically characterized an inducible lacz (BBa_K902090) circuit, replacing a faulty existing circuit in the registry containing a frameshift.
Electrochemically characterized a constitutive bglX (BBa_K902005) generator in terms of its electrochemical activity.
Tested one of our transposon library hits with our electrochemical reporter, showing that we can use our system to detect toxins electrochemically.
Validated our system with tailings using our transposon system and electrochemical reporter to be able to selectively detect toxins on real oil sands samples.
In terms of OSCAR, we...
Obtained additional characterization data in validation of our flux-variability analysis model.
Characterized the ability of our novel amdA (BBa_K902041) BioBrick to selectively remove primary amides from ring structures with remarkable efficiency, turning them into a substrate that the Petrobrick (BBa_K590025) can likely convert into hydrocarbons.
Validated a scale up model for using our bioreactor/belt skimmer system in producing and extracting hydrocarbons.
