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Team:Calgary/Project/Post-Regionals
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<li><p><a class="orange" href="https://2012.igem.org/Team:Calgary/Project/HumanPractices/Interviews"><b>Returned to industry experts</b></a> to see if we accomplished our goals and what the <a class="purple" href="https://2012.igem.org/Team:Calgary/Project/Synergy"><b>next steps</b></a> should be.</li></p> | <li><p><a class="orange" href="https://2012.igem.org/Team:Calgary/Project/HumanPractices/Interviews"><b>Returned to industry experts</b></a> to see if we accomplished our goals and what the <a class="purple" href="https://2012.igem.org/Team:Calgary/Project/Synergy"><b>next steps</b></a> should be.</li></p> | ||
| - | <li><p><a class="orange" href="https://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch/Regulation"><b>Characterized</b></a> the functionality of our previously submitted <a class="orange" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K902066"><b><i>Prha</i> (BBa_K902066)</b></a> promoter | + | <li><p><a class="orange" href="https://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch/Regulation"><b>Characterized</b></a> the functionality of our previously submitted <a class="orange" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K902066"><b><i>Prha</i> (BBa_K902066)</b></a> promoter using GFP fluorescence.</li></p> |
| - | <li><p>Tested an additional <b>complete kill switch device</b> using the <i>Prha</i> promoter with our S7 kill gene.</li></p> | + | <li><p>Tested an additional <a class="orange" href="https://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch/Regulation"><b>complete kill switch device</b></a> using the <a class="orange" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K902084"><b><i>Prha</i> (BBa_K902084)</b></a> promoter with our S7 kill gene.</li></p> |
| - | <li><p><b>Further characterized</b> our previously validated <b>magnesium riboswitch kill gene construct</b>.</li></p></ul> | + | <li><p><a class="orange" href="https://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch/Regulation"><b>Further characterized</b></a> our previously validated <b>magnesium riboswitch kill gene construct</b><a class="orange" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K902018"> <b>(BBa_K902018)</b></a>.</li></p></ul> |
<br> | <br> | ||
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<ul> | <ul> | ||
| - | <li><p><b>Electrochemically characterized</b> an inducible <b><i>lacz</i> | + | <li><p><a class="green" href="https://2012.igem.org/Team:Calgary/Project/FRED/Reporting"><b>Electrochemically characterized</b></a> an inducible <a class="green" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K902090"><b><i>lacz</i> (BBa_K902090)</b></a> circuit, replacing a faulty existing circuit in the registry containing a frameshift.</p></li> |
| - | <li><p><b>Electrochemically characterized</b> a constitutive <b>bglX generator</b> in terms of its electrochemical activity.</p></li> | + | <li><p><a class="green" href="https://2012.igem.org/Team:Calgary/Project/FRED/Reporting"><b>Electrochemically characterized</b></a> a constitutive <a class="green" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K902005"><b><i>bglX</i> (BBa_K902005)</b></a> <b>generator</b> in terms of its electrochemical activity.</p></li> |
| - | <li><p><b>Characterized</b> and demonstrated how <b>3 different hydrolase-producing circuits</b> can be used simultaneously in order to create a <b>multiplex biosensor.</b> </p></li> | + | <li><p><a class="green" href="https://2012.igem.org/Team:Calgary/Project/FRED/Reporting"><b>Characterized</b></a> and demonstrated how <b>3 different hydrolase-producing circuits</b> can be used simultaneously in order to create a <b>multiplex biosensor.</b> </p></li> |
| - | <li><p><b>Tested one of our transposon library hits</b> with our electrochemical reporter, showing that we can use our system to <b>detect toxins electrochemically</b>.</p></li> | + | <li><p><a class="purple" href="https://2012.igem.org/Team:Calgary/Project/Synergy"><b>Tested one of our transposon library hits</b></a> with our electrochemical reporter, showing that we can use our system to <b>detect toxins electrochemically</b>.</p></li> |
</ul> | </ul> | ||
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<p><b>In terms of <FONT COLOR=#1088CC>OCSAR</FONT>, we...</b></p> | <p><b>In terms of <FONT COLOR=#1088CC>OCSAR</FONT>, we...</b></p> | ||
<ul> | <ul> | ||
| - | <li><p>Obtained <b>additional characterization data</b> in validation of our flux-variability analysis model.</p></li> | + | <li><p>Obtained <a class="blue" href="https://2012.igem.org/Team:Calgary/Project/OSCAR/FluxAnalysis"><b>additional characterization data</b></a> in validation of our flux-variability analysis model.</p></li> |
<li><p>Characterized the ability of our <b>novel amidase BioBrick</b> to remove nitrogen atoms from ring structures with remarkeable efficiency, turning them into a substrate that the Petrobrick (BBa_K) can likely convert into hydrocarbons.</p></li> | <li><p>Characterized the ability of our <b>novel amidase BioBrick</b> to remove nitrogen atoms from ring structures with remarkeable efficiency, turning them into a substrate that the Petrobrick (BBa_K) can likely convert into hydrocarbons.</p></li> | ||
Revision as of 20:22, 26 October 2012
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Post-Regional Accomplishments
Our team has had many accomplishments since the regional jamboree!
In our Human Practices project, we...
Returned to industry experts to see if we accomplished our goals and what the next steps should be.
Characterized the functionality of our previously submitted Prha (BBa_K902066) promoter using GFP fluorescence.
Tested an additional complete kill switch device using the Prha (BBa_K902084) promoter with our S7 kill gene.
Further characterized our previously validated magnesium riboswitch kill gene construct (BBa_K902018).
In terms of FRED, we...
Electrochemically characterized an inducible lacz (BBa_K902090) circuit, replacing a faulty existing circuit in the registry containing a frameshift.
Electrochemically characterized a constitutive bglX (BBa_K902005) generator in terms of its electrochemical activity.
Characterized and demonstrated how 3 different hydrolase-producing circuits can be used simultaneously in order to create a multiplex biosensor.
Tested one of our transposon library hits with our electrochemical reporter, showing that we can use our system to detect toxins electrochemically.
In terms of OCSAR, we...
Obtained additional characterization data in validation of our flux-variability analysis model.
Characterized the ability of our novel amidase BioBrick to remove nitrogen atoms from ring structures with remarkeable efficiency, turning them into a substrate that the Petrobrick (BBa_K) can likely convert into hydrocarbons.
Tested out our bioreactor and it’s ability to grow cells containing the PetroBrick as well as extract the hydrocarbon layer produced using our belt skimmer
