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Team:NYMU-Taipei/ymip2.html
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| - | href="https://2012.igem.org/Team:NYMU-Taipei/ymip3.html">Reprogramming of Somatic | + | href="https://2012.igem.org/Team:NYMU-Taipei/ymip3.html">Reprogramming of Somatic Cells into Stem & Separation of iPS cells</a></li> |
| - | Cells into Stem | + | |
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Revision as of 03:41, 19 October 2012
Mesenchymal Stem Cells Culturing
- Mesenchymal stem cells from human umbilical cord were provided by Prof. Chun-Min Lo from National Yang Ming University.
- View cultures using a microscopy and after the cultures was full of the dish, remove the spent medium.
- Wash the cell monolayer with PBS and remove it.
- Add HyQTase(1ml per 25 cm2 of surface area) and incubate for 1 minutes.
- After making sure cells were detached, resuspend the cells with medium(more than 5 folds volume of HyQTase).
- Centrifuge under 2000 rpm, 5minutes, 25°C.
- Remove the supernatant and add 5 ml medium to suspend cells.
- Count the cells by Bright Line Counting Chamber. Subject the cell diversity to 104/ml
- Seed the cells for 104/well, 1ml/well in the 24-well plate.
- Until cells grow full of each well, do the coculturing experiment.
