"
Team:NYMU-Taipei/ymip2.html
From 2012.igem.org
(Difference between revisions)
| Line 79: | Line 79: | ||
<div id="ymi_left_column"> | <div id="ymi_left_column"> | ||
<div class="text_area" align="justify"> | <div class="text_area" align="justify"> | ||
| - | <div class="title"> | + | <div class="title">Mesenchymal Stem Cells Culturing</div> |
<div align="left"> | <div align="left"> | ||
| - | + | ||
</div> | </div> | ||
<div align="left"> | <div align="left"> | ||
Revision as of 03:33, 19 October 2012
Mesenchymal Stem Cells Culturing
- Mesenchymal stem cells from human umbilical cord were provided by Prof. Chun-Min Lo from National Yang Ming University.
- View cultures using a microscopy and after the cultures was full of the dish, remove the spent medium.
- Wash the cell monolayer with PBS and remove it.
- Add HyQTase(1ml per 25 cm2 of surface area) and incubate for 1 minutes.
- After making sure cells were detached, resuspend the cells with medium(more than 5 folds volume of HyQTase).
- Centrifuge under 2000 rpm, 5minutes, 25°C.
- Remove the supernatant and add 5 ml medium to suspend cells.
- Count the cells by Bright Line Counting Chamber. Subject the cell diversity to 104/ml
- Seed the cells for 104/well, 1ml/well in the 24-well plate.
- Until cells grow full of each well, do the coculturing experiment.
