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Team:Calgary/Notebook/Protocols/onculture
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<li> 10mL culture tube. Use 16mm x 160mm or 16mm x 125mm</li> | <li> 10mL culture tube. Use 16mm x 160mm or 16mm x 125mm</li> | ||
<li> 5 mL LB</li> | <li> 5 mL LB</li> | ||
| - | <li> 5 | + | <li> 5 μL 1000X antibiotics</li> |
<li> Single colonies on a plate (best not to start an over night from a glycerol stock)</li> | <li> Single colonies on a plate (best not to start an over night from a glycerol stock)</li> | ||
</ul> | </ul> | ||
Latest revision as of 03:31, 4 October 2012
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Overnight Cultures
Reagents and Materials (per culture prepared):
- 10mL culture tube. Use 16mm x 160mm or 16mm x 125mm
- 5 mL LB
- 5 μL 1000X antibiotics
- Single colonies on a plate (best not to start an over night from a glycerol stock)
Protocol
- Add 5mL sterile/autoclaved LB in a 10mL culture tube.
- Pipet 5μL of 1000X antibiotic into the LB.
- Select a single colony using a sterile toothpick or pipette tip.
- Place toothpick or pipette tip in the culture tube and stir.
- Remove toothpick, or in the case of a pipette tip, leave in the tube.
- Place culture tube in incubator at 37°C overnight shaking vigorously (250 RPM)
