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Team:Calgary/Notebook/Protocols/mgtacircuit
From 2012.igem.org
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| - | TITLE=Characterization of mgtA | + | TITLE=Characterization of the mgtA regulation with S7 killgene| |
CONTENT=<html> | CONTENT=<html> | ||
<ol> | <ol> | ||
<li>Cells with the appropriate circuits were grown in LB overnight with 10mM MgCl<font style="text-transform: lowercase;">2</font>.</li> | <li>Cells with the appropriate circuits were grown in LB overnight with 10mM MgCl<font style="text-transform: lowercase;">2</font>.</li> | ||
| - | <li>The next morning the cells were spun down and washed with M9 minimal media and appropriate amounts of MgCl<font style="text-transform: lowercase;">2</font>.</li> | + | <li>The next morning the cells were spun down and washed with <a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/m9media">M9 minimal media</a> and appropriate amounts of MgCl<font style="text-transform: lowercase;">2</font>.</li> |
<li>Cells were then aliquoted into 96 well plates in triplicates for each concentration and OD was measured at 600nm for every 4 hour for 8 hours.</li> | <li>Cells were then aliquoted into 96 well plates in triplicates for each concentration and OD was measured at 600nm for every 4 hour for 8 hours.</li> | ||
<li>Additionally, the cells were plated on the appropriate antibiotic in dilutions of 1:1000 and 1:10000 for measuring CFU.</li> | <li>Additionally, the cells were plated on the appropriate antibiotic in dilutions of 1:1000 and 1:10000 for measuring CFU.</li> | ||
Latest revision as of 02:19, 4 October 2012
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Characterization of the mgtA regulation with S7 killgene
- Cells with the appropriate circuits were grown in LB overnight with 10mM MgCl2.
- The next morning the cells were spun down and washed with M9 minimal media and appropriate amounts of MgCl2.
- Cells were then aliquoted into 96 well plates in triplicates for each concentration and OD was measured at 600nm for every 4 hour for 8 hours.
- Additionally, the cells were plated on the appropriate antibiotic in dilutions of 1:1000 and 1:10000 for measuring CFU.
