Team:Calgary/Notebook/Protocols/mgtacircuit

From 2012.igem.org

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<li>Cells were then aliquoted into 96 well plates in triplicates for each concentration and OD was measured at 600nm for every 4 hour for 8 hours.</li>
<li>Cells were then aliquoted into 96 well plates in triplicates for each concentration and OD was measured at 600nm for every 4 hour for 8 hours.</li>
<li>Additionally, the cells were plated on the appropriate antibiotic in dilutions of 1:1000 and 1:10000 for measuring CFU.</li>
<li>Additionally, the cells were plated on the appropriate antibiotic in dilutions of 1:1000 and 1:10000 for measuring CFU.</li>
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</html>}}

Revision as of 03:37, 2 October 2012

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Characterization of mgtA circuit with S7 promoter

  • Cells with the appropriate circuits were grown in LB overnight with 10mM MgCl2.
  • The next morning the cells were spun down and washed with M9 minimal media and appropriate amounts of MgCl2.
  • Cells were then aliquoted into 96 well plates in triplicates for each concentration and OD was measured at 600nm for every 4 hour for 8 hours.
  • Additionally, the cells were plated on the appropriate antibiotic in dilutions of 1:1000 and 1:10000 for measuring CFU.

    • Retrieved from "http://2012.igem.org/Team:Calgary/Notebook/Protocols/mgtacircuit"