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Team:Calgary/Notebook/Protocols
From 2012.igem.org
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<h2>General Protocols</h2> | <h2>General Protocols</h2> | ||
| - | <li><a href="https:// | + | <li><a href="https://2012.igem.org/Team:Calgary/Notebook/Protocols/plasmidminiprep">Plasmid Isolation (from <i>E. coli</i>)</a></li> |
<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process2">RNA Extraction</a></li> | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process2">RNA Extraction</a></li> | ||
<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process3">cDNA Synthesis</a></li> | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process3">cDNA Synthesis</a></li> | ||
Revision as of 00:49, 2 October 2012
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Protocols
Here is a list of all the procedures we used this summer. Each contains a description and list of materials required.
- Electrode Plating
- Buffer Preparation
- Electrical Settings
- Testing For Oxidation
- Plasmid Isolation (from E. coli)
- RNA Extraction
- cDNA Synthesis
- Quantitative Real Time PCR
- Testing the pBAD-lacZ Construct
- Taq PCR Protocol
- PCR Purification
- Gel Extraction
- Testing the lacI-lacZ I732901 Gene
- Bacterial Transformation
- Rehydration of Registry DNA
- Construction Techniques
- Agarose Gel Electrophresis
- LB Agar Plates
- Overnight Cultures
- Preparing Chemically Competent Cells (E. coli)
- Preparing Glycerol Stocks (E. coli)
