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Team:Calgary/Project/OSCAR
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</html>[[IMAGE:UCalgary2012_OSCAR+definition.png|320px|right]]<html> | </html>[[IMAGE:UCalgary2012_OSCAR+definition.png|320px|right]]<html> | ||
| - | <p>The <FONT COLOR=43BBFF>O</FONT>ptimized <FONT COLOR=43BBFF>S</FONT>ystem for <FONT COLOR=43BBFF>C</FONT>arboxylic <FONT COLOR=43BBFF>A</FONT>cid <FONT COLOR=43BBFF>R</FONT>emediation, or OSCAR, is the <i><b>Destroy</i></b> component to our iGEM 2012 Calgary project. Building on last years biosensor, OSCAR converts naphthenic acids (NAs) into hydrocarbons by removing unwanted functional groups | + | <p>The <FONT COLOR=43BBFF>O</FONT>ptimized <FONT COLOR=43BBFF>S</FONT>ystem for <FONT COLOR=43BBFF>C</FONT>arboxylic <FONT COLOR=43BBFF>A</FONT>cid <FONT COLOR=43BBFF>R</FONT>emediation, or OSCAR, is the <i><b>Destroy</i></b> component to our iGEM 2012 Calgary project. Building on last years biosensor, OSCAR converts naphthenic acids (NAs) into hydrocarbons by removing unwanted carboxylic acid functional groups from their intricate chemical structures. By creating hydrocarbons we can provide not only detoxify tailings waters but provide an economically viable method for doing so. To further characterize this potential our group aimed to also convert catechol into less toxic more valuable components. By using flux balance analysis we developed a system to optimize the output of carboxylic acid removal system which we validated in the wetlab. Furthermore we developed a bioreactor prototype to demonstrate the applicability of our system using novel hydrocarbon collection methodologies. Finally, we developed constructs and genetic circuits to upgrade these hydrocarbons to reduce sulfur and nitrogen content. Altogether, OSCAR provides a method to upgrade naphthenic acids and other toxic components from waste products into useable fuels.</p> |
<h2>Overview of OSCAR Removal Pathways ⎯ OSCAR in 4D</h2> | <h2>Overview of OSCAR Removal Pathways ⎯ OSCAR in 4D</h2> | ||
Revision as of 04:21, 1 October 2012
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OSCAR
The Optimized System for Carboxylic Acid Remediation, or OSCAR, is the Destroy component to our iGEM 2012 Calgary project. Building on last years biosensor, OSCAR converts naphthenic acids (NAs) into hydrocarbons by removing unwanted carboxylic acid functional groups from their intricate chemical structures. By creating hydrocarbons we can provide not only detoxify tailings waters but provide an economically viable method for doing so. To further characterize this potential our group aimed to also convert catechol into less toxic more valuable components. By using flux balance analysis we developed a system to optimize the output of carboxylic acid removal system which we validated in the wetlab. Furthermore we developed a bioreactor prototype to demonstrate the applicability of our system using novel hydrocarbon collection methodologies. Finally, we developed constructs and genetic circuits to upgrade these hydrocarbons to reduce sulfur and nitrogen content. Altogether, OSCAR provides a method to upgrade naphthenic acids and other toxic components from waste products into useable fuels.
Overview of OSCAR Removal Pathways ⎯ OSCAR in 4D
Decarboxylation
Functional group. Pathway. Progress
Desulfurization
Functional group. Pathway. Progress
Denitrogenation
Functional group. Pathway. Progress
Decatecholization
Functional group. Pathway. Progress
Bioreactor: The House of OSCAR
Design.Prototype
Flux-Balance Modelling for OSCAR Optimization
Model. Software
