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Team:NYMU-Taipei/ymico1.html
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<p>Since long ago, We’ve had close relationship with NTU-Taida iGEMers. By inviting them for a iGEMers fest, meeting with each other, technique assistance and having FUN together, we elevate the quality of our projects. As a result, we, NMYU-Taipei team collaborated with NTU-Taida again!!<br /> | <p>Since long ago, We’ve had close relationship with NTU-Taida iGEMers. By inviting them for a iGEMers fest, meeting with each other, technique assistance and having FUN together, we elevate the quality of our projects. As a result, we, NMYU-Taipei team collaborated with NTU-Taida again!!<br /> | ||
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| - | In similar, both NMYU-Taipei and NTU-Taida team did bacteria engineering. In our projects, we utilized cyanobacteria to remove several pollutants inside our environment. Moreover, we try to achieve Venusian goals. Fortunately, NTU-Taida had a powerful promoter, a thermosensitive promoter, phs, which is suitable for gene expression on Venus (high temperature environment). So NTU-Taida help us to create two new plasmid, phs-CysI and phs-SQR. </p><div class=out style='text-align:center'><img src=" | + | In similar, both NMYU-Taipei and NTU-Taida team did bacteria engineering. In our projects, we utilized cyanobacteria to remove several pollutants inside our environment. Moreover, we try to achieve Venusian goals. Fortunately, NTU-Taida had a powerful promoter, a thermosensitive promoter, phs, which is suitable for gene expression on Venus (high temperature environment). So NTU-Taida help us to create two new plasmid, phs-CysI and phs-SQR. </p><div class=out style='text-align:center'><img src="https://static.igem.org/mediawiki/2012/a/a0/Ymico2.gif" alt="" width="425" height="321" /><br /> |
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In NTU-Taida’s project, they want to create a impotent E.coli which help people to lost weight. The gene express function is quite important in their artificial E.coli. That’s the reason why we help NTU-Taida to quality control their bacteria. To test the plasmid removal rate of bacteria the incubated three plasmid transformed E.coli, IR, tnpR, SmBC and diluted in to 10-7, 10-8, 10-9 three different concentration. Then we incubated E.cloi in LB solid agar plate and calculated the colonies of each group. We found out that the same result we got as NTU-Taida did. That is to say, they got a reliable data, also we successfully quality controlled of their E.coli.<br /> | In NTU-Taida’s project, they want to create a impotent E.coli which help people to lost weight. The gene express function is quite important in their artificial E.coli. That’s the reason why we help NTU-Taida to quality control their bacteria. To test the plasmid removal rate of bacteria the incubated three plasmid transformed E.coli, IR, tnpR, SmBC and diluted in to 10-7, 10-8, 10-9 three different concentration. Then we incubated E.cloi in LB solid agar plate and calculated the colonies of each group. We found out that the same result we got as NTU-Taida did. That is to say, they got a reliable data, also we successfully quality controlled of their E.coli.<br /> | ||
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| - | <div class=out style='text-align:center'><img src=" | + | <div class=out style='text-align:center'><img src="https://static.igem.org/mediawiki/2012/4/49/Ymico3.gif" alt="" width="554" height="589" /><br /> |
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The result indicates that tnpR, SmBC transformed E.coli have much more E.coli colonies, compared to the IR tansformed E.coli. In other words, IR have higher removal rate that is the same as our expected. </p> | The result indicates that tnpR, SmBC transformed E.coli have much more E.coli colonies, compared to the IR tansformed E.coli. In other words, IR have higher removal rate that is the same as our expected. </p> | ||
Revision as of 02:03, 27 September 2012
Extras
Collaboration with NTU
Since long ago, We’ve had close relationship with NTU-Taida iGEMers. By inviting them for a iGEMers fest, meeting with each other, technique assistance and having FUN together, we elevate the quality of our projects. As a result, we, NMYU-Taipei team collaborated with NTU-Taida again!!
In similar, both NMYU-Taipei and NTU-Taida team did bacteria engineering. In our projects, we utilized cyanobacteria to remove several pollutants inside our environment. Moreover, we try to achieve Venusian goals. Fortunately, NTU-Taida had a powerful promoter, a thermosensitive promoter, phs, which is suitable for gene expression on Venus (high temperature environment). So NTU-Taida help us to create two new plasmid, phs-CysI and phs-SQR.
In NTU-Taida’s project, they want to create a impotent E.coli which help people to lost weight. The gene express function is quite important in their artificial E.coli. That’s the reason why we help NTU-Taida to quality control their bacteria. To test the plasmid removal rate of bacteria the incubated three plasmid transformed E.coli, IR, tnpR, SmBC and diluted in to 10-7, 10-8, 10-9 three different concentration. Then we incubated E.cloi in LB solid agar plate and calculated the colonies of each group. We found out that the same result we got as NTU-Taida did. That is to say, they got a reliable data, also we successfully quality controlled of their E.coli.
The result indicates that tnpR, SmBC transformed E.coli have much more E.coli colonies, compared to the IR tansformed E.coli. In other words, IR have higher removal rate that is the same as our expected.
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Extras
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Protocol
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Notebook
