Team:NYMU-Taipei/ymijf.html

From 2012.igem.org

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   <p><strong><em>Into the cell</em></strong>
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   <p><img src="https://static.igem.org/mediawiki/igem.org/c/c8/Ymicheck.gif" alt=""width="35" /> We have already <a href="https://igem.org/Team_List?year=2012"><b>registered</b></a> a team, work hard in summer, </p>
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  <p style="margin-left:40px;">and ready to join Jamboree!!.</p>
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  <br />
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  <p><img src="https://static.igem.org/mediawiki/igem.org/c/c8/Ymicheck.gif" alt=""width="35" /> We have already completed and submit our judging form.</p>
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  <br />
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  <p><img src="https://static.igem.org/mediawiki/igem.org/c/c8/Ymicheck.gif" alt=""width="35" /> We have not only finished our project description on <a href="https://2012.igem.org/Team:NYMU-Taipei"><b>iGEM wiki</b></a>, </p>
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  <p style="margin-left:40px;">but also completed 80% of our big plan!!</p>
 +
  <br />
 +
  <p><img src="https://static.igem.org/mediawiki/igem.org/c/c8/Ymicheck.gif" alt=""width="35" /> We have well prepared for presentation in iGEM Jamboree and be sure, </p>
 +
  <p style="margin-left:40px;">it’s gonna be a fantastic one!!</p>
 +
  <br />
 +
  <p><img src="https://static.igem.org/mediawiki/igem.org/c/c8/Ymicheck.gif" alt=""width="35" /> In our project, we have already established several standard <a href="https://2012.igem.org/Ymiparts.html"><b>BioBrick Parts </b></a></p>
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  <p style="margin-left:40px;">in the Registry of Standard Biological Parts.</p>
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  <br />
 +
  <p><img src="https://static.igem.org/mediawiki/igem.org/c/c8/Ymicheck.gif" alt=""width="35" /> In our project, we’ve already performed experiments to demonstrate </p>
 +
  <p style="margin-left:40px;">that our new BioBrick Part work!! For example, <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K896000"><b>SQR(sulfide quinone reductase, </b></a></p>
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  <p style="margin-left:40px;"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K896000"><b>BBa_K896000) </b></a>, <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K896001"><b>CysI(surfur reductase, BBa_K896001) </b></a>, and <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K896002"><b>Dsr (surfur reductase, </b></a></p>
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  <p style="margin-left:40px;"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K896002"><b>BBa_K896002) </b></a>!!</p>
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<br />
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  <p><img src="https://static.igem.org/mediawiki/igem.org/c/c8/Ymicheck.gif" alt=""width="35" /> We have characterised several of our <a href="https://2012.igem.org/Ymiparts.html"><b>BioBricks</b></a>. </p>
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  <p style="margin-left:40px;">Our favourites are <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K896000" ><b>SQR(sulfide quinone reductase, BBa_K896000) </b></a>,</p>
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  <p style="margin-left:40px;"><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K896001"><b>CysI(surfur reductase, BBa_K896001) </b></a>, and <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K896002"><b>Dsr (surfur reductase, BBa_K896002) </b></a></p>
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  <p style="margin-left:40px;">We’ve already characterized the operation of your new part/device, such as decrease </p>
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  <p style="margin-left:40px;">SOX, NOX in the environment, create a new bioremediation with our artificial cyanobacteria.</p>
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  <p style="margin-left:40px;">Fortunately, they are the same as our expected!!<br />
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  </p>
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    We construct a plasmid that contains LLO, invasin, and mRFP. Then  add the transgenic E.coli (absorbance of  0.5 at OD600, 1ml ) into the medium of amoeba (10ml) and let stand for 1,4, 8, 12, 24 hours.<br />
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  <p><img src="https://static.igem.org/mediawiki/igem.org/c/c8/Ymicheck.gif" alt=""width="35" /> We’ve already modified several existing BioBrick Part, and used experiment </p>
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   <img src="images/cdf.gif" alt=" " width="407" height="187" /></p>
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  <p style="margin-left:40px;">to prove the function<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K896012"><b>Inv+LLO+RFP (reporter for invasin &amp; listeriolysin) BBa_K896012</b></a>. </p>
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   <p>Then we observe the amoeba with fluorescent  microscope to see if there is any E.coli inside of amoeba. We also compared the  fluorescent between amoeba cell that were co-cultured with the cell. To make  sure the E.coli really stay INSIDE of amoeba. We use centrifugation to confirm  our observation. Dictystelium discoideum in ML-5 medium should be settled with  1000 x g for 4 min and E.coli should not be settled beneath 2000 x g for 5min.  We will test this statement. If it is true, then we will spin down the liquid  medium containing amoeba and E.coli at 1000 x g for 4 min. Two types of E.coli  will be tested. One can express LLO, invasin, and GFP; the other express GFP only.  Discard the supernatant after centrifugation and wash the  pellet with ML-5 medium. Repeat  three times. Then we can use the spectrometer to compare the fluorescence in  the pellet.</p>
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  <p style="margin-left:40px;">We successfully fused the old one with our project and further elevated our idea to</p>
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  <p style="margin-left:40px;"> a higher level, to create venusian!!</p>
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  <br />
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   <p><strong><em>Kill-switch of  amoeba<br />
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   <p><img src="https://static.igem.org/mediawiki/igem.org/c/c8/Ymicheck.gif" alt=""width="35" /> We have already cooperated with <a href="https://2012.igem.org/Ymico.html"><b>NTU-Taida </b></a>!! not only to further</p>
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        <br />
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   <p style="margin-left:40px;"> accelerated our progression, also debugged with each other for win-win situation!!</p>
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   </em></strong>We cloned and tested  the function of zinTp. The transgenic E.coli contains zinTp+GFP, which should fluoresce  when Cd2+ exist. We added different concentration of cadmium ion into LB medium  and compared the fluorescence level of E.coli which contain zinTp+GFP,  pTetR+GFP, and wildtype E.coli.
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  <br />
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</p>
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  <p><img src="https://static.igem.org/mediawiki/igem.org/c/c8/Ymicheck.gif" alt=""width="35" /> Our team members are absolutely glad to help other iGEMers!! We’ve already shared our </p>
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</div>
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   <p style="margin-left:40px;">experiences with NTU-Taida. Also, we’ve held a <a href="https://2012.igem.org/Ymihpa.html"><b>meet-up </b></a> with invited NTU-Taida and </p>
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   <p style="margin-left:40px;">NCTU_Formosa iGEMers.</p>
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   <p><strong><em>Tolerance to cadmium ions</em></strong><br />
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    <br />
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  <p><img src="https://static.igem.org/mediawiki/igem.org/c/c8/Ymicheck.gif" alt=""width="35" /> Human practice is the most colorful part inside our project!! </p>
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    We compared the tolerance level  between non-transgenic E.coli and transgenic E.coli. We tested the growth curve  of E.coli with different cadmium concentration.  </p>
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  <p style="margin-left:40px;">We have already outlined and detailed a extremely new human practice-</p>
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   <p style="margin-left:40px;"><a href="https://2012.igem.org/Ymihph.html"><b>outer space human practice project </b></a> , which is the newest one among all other  teams!! </p>
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  <p style="margin-left:40px;">Also, we create new approaches for learning synthetic biology. </p>
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Revision as of 00:35, 27 September 2012

NYMU iGEM

Extras

Achievements


We have already registered a team, work hard in summer,

and ready to join Jamboree!!.


We have already completed and submit our judging form.


We have not only finished our project description on iGEM wiki,

but also completed 80% of our big plan!!


We have well prepared for presentation in iGEM Jamboree and be sure,

it’s gonna be a fantastic one!!


In our project, we have already established several standard BioBrick Parts

in the Registry of Standard Biological Parts.


In our project, we’ve already performed experiments to demonstrate

that our new BioBrick Part work!! For example, SQR(sulfide quinone reductase,

BBa_K896000) , CysI(surfur reductase, BBa_K896001) , and Dsr (surfur reductase,

BBa_K896002) !!


We have characterised several of our BioBricks.

Our favourites are SQR(sulfide quinone reductase, BBa_K896000) ,

CysI(surfur reductase, BBa_K896001) , and Dsr (surfur reductase, BBa_K896002)

We’ve already characterized the operation of your new part/device, such as decrease

SOX, NOX in the environment, create a new bioremediation with our artificial cyanobacteria.

Fortunately, they are the same as our expected!!

We’ve already modified several existing BioBrick Part, and used experiment

to prove the functionInv+LLO+RFP (reporter for invasin & listeriolysin) BBa_K896012.

We successfully fused the old one with our project and further elevated our idea to

a higher level, to create venusian!!


We have already cooperated with NTU-Taida !! not only to further

accelerated our progression, also debugged with each other for win-win situation!!


Our team members are absolutely glad to help other iGEMers!! We’ve already shared our

experiences with NTU-Taida. Also, we’ve held a meet-up with invited NTU-Taida and

NCTU_Formosa iGEMers.


Human practice is the most colorful part inside our project!!

We have already outlined and detailed a extremely new human practice-

outer space human practice project , which is the newest one among all other teams!!

Also, we create new approaches for learning synthetic biology.

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