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Team:Bordeaux/Third
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<li><a href="https://2012.igem.org/Team:Bordeaux/Fourth">Fourth Week</a></li> | <li><a href="https://2012.igem.org/Team:Bordeaux/Fourth">Fourth Week</a></li> | ||
<li><a href="https://2012.igem.org/Team:Bordeaux/Fifth">Fifth Week</a></li> | <li><a href="https://2012.igem.org/Team:Bordeaux/Fifth">Fifth Week</a></li> | ||
| + | <li><a href="https://2012.igem.org/Team:Bordeaux/Sixth">Sixth Week</a></li> | ||
| + | <li><a href="https://2012.igem.org/Team:Bordeaux/Seventh">Seventh Week</a></li> | ||
| + | <li><a href="https://2012.igem.org/Team:Bordeaux/Eighth">Eighth Week</a></li> | ||
| + | <li><a href="https://2012.igem.org/Team:Bordeaux/Ninth">Ninth Week</a></li> | ||
| + | <li><a href="https://2012.igem.org/Team:Bordeaux/Tenth">Tenth Week</a></li> | ||
| + | <li><a href="https://2012.igem.org/Team:Bordeaux/Eleventh">Eleventh Week</a></li> | ||
| + | <li><a href="https://2012.igem.org/Team:Bordeaux/Twelfth">Twelfth Week</a></li> | ||
| + | <li><a href="https://2012.igem.org/Team:Bordeaux/Thirteenth">Thirteenth Week</a></li> | ||
| + | |||
| + | |||
</ul> | </ul> | ||
</div> | </div> | ||
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We followed the protocol proposed by the patregistry.org <a href="http://partsregistry.org/Help:Protocols/Competent_Cells" target="_blanck" class="more">Read Protocol</a> | We followed the protocol proposed by the patregistry.org <a href="http://partsregistry.org/Help:Protocols/Competent_Cells" target="_blanck" class="more">Read Protocol</a> | ||
</p> | </p> | ||
| + | |||
| + | <h4>Day 11 : 17-07-2012</h4> | ||
| + | <hr> | ||
| + | <p> | ||
| + | Day 2 of making new competent cells <a href="http://partsregistry.org/Help:Protocols/Competent_Cells" target="_blanck" class="more">Read Protocol</a> | ||
| + | </br> | ||
| + | We stopped incubating our cells at an OD600nm of 0.31 | ||
| + | </br> | ||
| + | In the end we made 192 tubes of 50μL competent cells. | ||
| + | </p> | ||
| + | |||
| - | <h4>Day | + | <h4>Day 12 : 18-07-2012</h4> |
<hr> | <hr> | ||
<p> | <p> | ||
| - | The competence test gave good results with a good amount of colonies on each plates and no colonies on the control plate. | + | The competence test gave good results with a good amount of colonies on each plates ( ≈ 300 ) and no colonies on the control plate. |
</br> | </br> | ||
Today we will transform DNA for every biobrick left. | Today we will transform DNA for every biobrick left. | ||
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</p> | </p> | ||
| - | <h4>Day | + | <h4>Day 13 : 19-07-2012</h4> |
<hr> | <hr> | ||
<p> | <p> | ||
| + | Today we picked single colonies and put the to grow in two 5 mL LB + Ampicilin 100μg/mL | ||
| + | </br> | ||
| + | Except for 3.B who gave no colonies | ||
| + | </br> | ||
| + | We put the tubes to incubate overnight at 37°C | ||
</p> | </p> | ||
| - | + | <h4>Day 14 : 20-07-2012</h4> | |
| + | <hr> | ||
| + | <p> | ||
| + | After 13 hours incubating we did a miniprep with the tubes. We got really poor results on the nanodrop for every biobrick (>3ng/μL). As we had really small cells pellets after the first centrifugation we suspect we didn't have enough cells in the beginning. | ||
| + | </p> | ||
</div> | </div> | ||
Latest revision as of 18:47, 26 September 2012
Day 10 : 16-07-2012
This week we decided to make our own competent cells Regarding the bad/average results we had with our commercial DH5α and XL1blue we decided to try with DH10B strain We followed the protocol proposed by the patregistry.org Read Protocol
Day 11 : 17-07-2012
Day 2 of making new competent cells Read Protocol We stopped incubating our cells at an OD600nm of 0.31 In the end we made 192 tubes of 50μL competent cells.
Day 12 : 18-07-2012
The competence test gave good results with a good amount of colonies on each plates ( ≈ 300 ) and no colonies on the control plate. Today we will transform DNA for every biobrick left. We will use our new competent cells.
We will proceed by heat-shock as described in the partregistry.org protocol Read Protocol
Day 13 : 19-07-2012
Today we picked single colonies and put the to grow in two 5 mL LB + Ampicilin 100μg/mL Except for 3.B who gave no colonies We put the tubes to incubate overnight at 37°C
Day 14 : 20-07-2012
After 13 hours incubating we did a miniprep with the tubes. We got really poor results on the nanodrop for every biobrick (>3ng/μL). As we had really small cells pellets after the first centrifugation we suspect we didn't have enough cells in the beginning.
