Note Book - iGEM Bordeaux 2012

Day 39 : 27-08-2012

Today we put the transformants (5 coloines per plate ) on LB precuture.

Day 40 : 28-08-2012

Today we did a miniprep of the precultures.
And we runned a gel to test the ligations.
IIIAIIB , 5AIVB and IIIDIIE are OK but ICIE will have to be redone.

Day 41 : 29-08-2012

Today we did ligations for IVA5AIVB , 5AIVDIVEIVC , IIAIIBIIC(1)5B ,IAIB , 5AIIIC , ICIE.
We inactivated the ligase after 1 hour 20°C incubation and put the samples on the fridge overnight.

Day 42 : 30-08-2012

Today we purified and transformed the ligations by electoporation.

Day 43 : 31-08-2012

Today we put the transformants into a LB preculture and put them at room temperature over the weekend.