Note Book - iGEM Bordeaux 2012

Day 47 : 11-09-2012

Today we put the transformants into LB preculture.

Day 48 : 12-09-2012

Today we did a miniprep of the precultures and tested the plasmids on agarose gel.

Every brick is Ok The ligations didn't worked well the only good one is 5AIIIC.

Day 49 : 14-09-2012

Today we digested the bricks needed for the ligations IAIB , IA'IB' , ICIE , IVAIIC5B , 5AIIICIIIEIIID , 5AIVDIVEIVC , IA IIC5B , IVA5AIVB , IVEIVC
We decided to try new assemblies for a two operons system because of the little time left we will not be able to complete the 3 operons project