Note Book - iGEM Bordeaux 2012

Day 44 : 03-09-2012

Today we did a miniprep of the precultures and runned a gel to test the plasmids.

Operon 2 completed !
But IVA5AIVB , 5AIIIC , IAIB ,ICIE and 5AIVDIVEIVC have to be redone.

Day 45 : 06-09-2012

Today we did the ligations for IVA5AIVB , 5AIVDIVEIVC and 5AIIIC
then inactivated the ligase after 1h 20 °C incubation and put overnight at 4°C

Day 46 : 07-09-2012

We transformed the ligations from yesterday plus biobricks IA ,IB ,IC ,IE and two new bricks IA' and IB' to make a new stock
We then put the plate at room temperature over the weekend