<ul><ul><img src="https://static.igem.org/mediawiki/2012/4/49/1_mod.png" alt="" /> Is the sensitivity of the amplification module satisfying regarding to the quantity of adenylate cyclase that the signaling module can create?</ul></ul>

<ul><ul><img src="https://static.igem.org/mediawiki/2012/1/1e/2_mod.png" alt="" /> What is the time of answer of our system?

</ul></ul>

<h1><img src="https://static.igem.org/mediawiki/2012/4/49/1_mod.png" alt="" /> Sensitivity </h1>

In the amplification module, we saw that we could get a signal if there was more than 10<SUP>-6</SUP> mol/L of initial cAMP. In the signaling part, we saw that when the dipeptide is detected, it will lead to the production of adenylate cyclase. Then, when the adenylate cyclase is produced, it leads to the production of cAMP, and this will launch the amplification module. Thus, we will have:

We notice that for an initial concentration of dipeptide around 10<SUP>-8,5</SUP> mol/L of dipeptide we can get a signal. If we make the conversion in number of molecule (we multiply by the Avogadro number (=6,02*10<SUP>23</SUP> mol/L) and by the volume of the cell (given in the quorum sensing part = 10<SUP>-15</SUP> L), we obtain that our detection threshold is around 20 molecules.

<h1><img src="https://static.igem.org/mediawiki/2012/1/1e/2_mod.png" alt="" /> Temporal evolution</h1>

Once activated, we need to know the time we will have to wait to start up the amplification module. We take the equations of the signaling module, when the production of adenylate cyclase is activated. We have: