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Team:Groningen/Notebook/Wetwork 14September2012
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3 constructs of alsT with amilgfp, amilcp and lycopene in psb1c3. 3 constructs of fnr with amilgfp, amilcp and lycopene in psb1c3. 4 constructs of sboA with 2x amilgfp,amilcp and lycopene in psb1c3.<br> | 3 constructs of alsT with amilgfp, amilcp and lycopene in psb1c3. 3 constructs of fnr with amilgfp, amilcp and lycopene in psb1c3. 4 constructs of sboA with 2x amilgfp,amilcp and lycopene in psb1c3.<br> | ||
| - | Transformation in <i>E. coli</i> did not work probably due to bad competent cells.<br> | + | Transformation in <i>E. coli</i> did not work probably due to bad competent cells.<br> <br> |
| + | Nisa <br><br> | ||
| + | Plasmid isolation results: some E. coli containing volatile detection device showed very strong colour. We also isolated positive control of GFP expression that has been provided by Jeroen (rrnB promoter+GFP)<br> <br> | ||
| + | Cloned strong promoter rrnB to pSac-Cm+GFP opt. <br> | ||
<br> | <br> | ||
<A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A> | <A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A> | ||
Latest revision as of 15:11, 26 September 2012
Tom
Restriction and ligation of constructs and linearized psb1c3 for submitting of parts.
3 constructs of alsT with amilgfp, amilcp and lycopene in psb1c3. 3 constructs of fnr with amilgfp, amilcp and lycopene in psb1c3. 4 constructs of sboA with 2x amilgfp,amilcp and lycopene in psb1c3.
Transformation in E. coli did not work probably due to bad competent cells.
Nisa
Plasmid isolation results: some E. coli containing volatile detection device showed very strong colour. We also isolated positive control of GFP expression that has been provided by Jeroen (rrnB promoter+GFP)
Cloned strong promoter rrnB to pSac-Cm+GFP opt.
Back to notebook
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