Team:Groningen/Notebook/Wetwork 5August2012

From 2012.igem.org

(Difference between revisions)
 
(One intermediate revision not shown)
Line 1: Line 1:
{{HeaderGroningen2012}}
{{HeaderGroningen2012}}
 +
<html>
 +
<head>
 +
<style type="text/css">
 +
p.margin
 +
{
 +
font-size:12pt;
 +
line-height:14pt;
 +
color:white;
-
<div style="margin-left: 100px; margin-right:100px; color:white; font-size:12pt;">
+
margin-top:0px;
 +
margin-bottom:20px;
 +
margin-left:150px;
 +
margin-right:250px;
 +
}
 +
 +
</style>
 +
</head>
 +
 +
<body><br><br><br>
 +
<p class="margin">
Nisa / Emeraldo<br><br>
Nisa / Emeraldo<br><br>
Line 14: Line 32:
6.) pSac-CM + lycopene<br>
6.) pSac-CM + lycopene<br>
7.) all pigments from Uppsala 2011 (amilCP+rbs, AmilGFP, AmilCP)<br>
7.) all pigments from Uppsala 2011 (amilCP+rbs, AmilGFP, AmilCP)<br>
-
 
+
<br>
-
PCR the BBa_B0015 with BioBrick prefix and suffix again, ligate into pSac-Cm MCS.
+
PCR the BBa_B0015 with BioBrick prefix and suffix again, ligate into pSac-Cm MCS.<br>
-
<html>
+
<br>
-
<div style="position:absolute; left: 100px; top: 500px; z-index:-1;">
+
<img src="https://static.igem.org/mediawiki/2012/b/b5/Groningen_RR_20120806_TAMC.png" width="450" height="320">
<img src="https://static.igem.org/mediawiki/2012/b/b5/Groningen_RR_20120806_TAMC.png" width="450" height="320">
-
</div>
+
<br>
 +
<A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A>
 +
</p><br><br>
 +
</body>
</html>
</html>
 +
 +
{{Template:SponsorsGroningen2012}}

Latest revision as of 13:14, 26 September 2012







Nisa / Emeraldo

Plasmid isolation from E. coli:
1.) 4 colonies alsT (150) promoter + GFP in pSB1C3
2.) 4 colonies alsT (250) promoter + GFP in pSB1C3
3.) 4 colonies alsT (300) promoter + GFP in pSB1C3
4.) 4 colonies alsT (500) promoter + GFP in pSB1C3
5.) 2 colonies of LacI promoter in pSB1C3
6.) pSac-CM + lycopene
7.) all pigments from Uppsala 2011 (amilCP+rbs, AmilGFP, AmilCP)

PCR the BBa_B0015 with BioBrick prefix and suffix again, ligate into pSac-Cm MCS.


Back to notebook



Our sponsors:

Retrieved from "http://2012.igem.org/Team:Groningen/Notebook/Wetwork_5August2012"