Team:Groningen/Notebook/Wetwork 5August2012

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Nisa / Emeraldo<br><br>
Nisa / Emeraldo<br><br>
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Plasmid isolation from E. coli:
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Plasmid isolation from E. coli:<br>
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1.) 4 colonies alsT (150) promoter + GFP in pSB1C3
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1.) 4 colonies alsT (150) promoter + GFP in pSB1C3<br>
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2.) 4 colonies alsT (250) promoter + GFP in pSB1C3
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2.) 4 colonies alsT (250) promoter + GFP in pSB1C3<br>
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3.) 4 colonies alsT (300) promoter + GFP in pSB1C3
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3.) 4 colonies alsT (300) promoter + GFP in pSB1C3<br>
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4.) 4 colonies alsT (500) promoter + GFP in pSB1C3
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4.) 4 colonies alsT (500) promoter + GFP in pSB1C3<br>
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5.) 2 colonies of LacI promoter in pSB1C3
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5.) 2 colonies of LacI promoter in pSB1C3<br>
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6.) pSac-CM + lycopene
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6.) pSac-CM + lycopene<br>
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7.) all pigments from Uppsala 2011 (amilCP+rbs, AmilGFP, AmilCP)
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7.) all pigments from Uppsala 2011 (amilCP+rbs, AmilGFP, AmilCP)<br>
PCR the BBa_B0015 with BioBrick prefix and suffix again, ligate into pSac-Cm MCS.
PCR the BBa_B0015 with BioBrick prefix and suffix again, ligate into pSac-Cm MCS.
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<img src="https://static.igem.org/mediawiki/2012/b/b5/Groningen_RR_20120806_TAMC.png" width="450" height="320">
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Revision as of 13:31, 25 September 2012





Nisa / Emeraldo

Plasmid isolation from E. coli:
1.) 4 colonies alsT (150) promoter + GFP in pSB1C3
2.) 4 colonies alsT (250) promoter + GFP in pSB1C3
3.) 4 colonies alsT (300) promoter + GFP in pSB1C3
4.) 4 colonies alsT (500) promoter + GFP in pSB1C3
5.) 2 colonies of LacI promoter in pSB1C3
6.) pSac-CM + lycopene
7.) all pigments from Uppsala 2011 (amilCP+rbs, AmilGFP, AmilCP)

PCR the BBa_B0015 with BioBrick prefix and suffix again, ligate into pSac-Cm MCS.

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