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From 2012.igem.org

Plasmid isolation of psaccm-eforRed, cut with EcoRI and PstI for insert check, cut with EcoRI and XbaI for ligation reaction later with cut PwapA and PsboA with EcoRI and SpeI. Cut amilGFP with EcoRI and XbaI for ligation with PwapA. Ligation reaction of PwapA with eforRed and amilGFP, PsboA with eforRed. Transformation of E. coli with ligated product.

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