Team:Grenoble/Project/Main Results
From 2012.igem.org
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As you can see the paraBAD works as expected. | As you can see the paraBAD works as expected. | ||
We began the construction of the amplification loop (paraBAD_gfp_cyaA). | We began the construction of the amplification loop (paraBAD_gfp_cyaA). |
Revision as of 23:42, 26 September 2012
Main results
In a first place, biologists and modellers worked on a simple amplification loop.
The modellers proved that a simple amplification loop could not work. There is no state where GFP is not expressed.
Modelling results confirmed that built system will work.
A possible track would be to add another regulation system on the adenylate cyclase production with the riboswitch RsmA/rsmY.
References
[1]Accumulation of Toxic Concentrations of Methylglyoxal by Wild-Type Escherichia coli K-12, Rollie S. Ackerman, Nicholas R. Cozzarelli and Wolfgang Epstein[2]Metabolic flux analysis of Escherichia coli in glucose-limited continuous culture. II. Dynamic response to famine and feast, activation of the methylglyoxal pathway and oscillatory behaviour Jan Weber†, Anke Kayser‡ and Ursula Rinas