Team:Grenoble/Modeling/Amplification/Stochastic/results

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iGEM Grenoble 2012

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Goal


In this part we would like to answer 2 questions thanks to the stochastic modeling.

      Is the sensibility given by stochastic modeling the same that in ODE modeling ?
      What is the part of false positives ?
Thanks to those 2 questions we will be able to establish if our device is still performing when we take into account random variations.

Sensibility


The ODE modeling gave 10-6 molecules of CAMPi as the sensibility. This means, if we have 10-6 molecules of CAMP at the initial point, the system will turn on. But this result is given by a deterministic analysis. What happens if we take into account the random phenomena of the bacterium ? Is the sensibility still so good ?

To perform that study, we use a Gillespie Algorithm to add randomness in our system. We want to obtain the evolution of the output signal (CA or GFP) depending on the concentration of the input signal (CAMPi) after 6h40 (400 min). To get that graph we simulate the algorithm hundreed times for each concentration of CAMPi (1, 5, 10, …, 100 000 molecules).

False positives

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