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Team:Grenoble/Biology/Protocols/Medium
From 2012.igem.org
(Difference between revisions)
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<table> | <table> | ||
<tr> | <tr> | ||
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<td>250 µL</td> | <td>250 µL</td> | ||
</table> | </table> | ||
| + | <br/> | ||
| + | The composition for a <b>2X</b> solution of the salts is the following: | ||
| + | <br/> | ||
| + | <br/> | ||
| + | <table> | ||
| + | <tr> | ||
| + | <td></td> | ||
| + | <th>Amount for 1L in g</th> | ||
| + | <th>FW</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>Na<span class="indice">2</span>HPO<span class="indice">4</span> x 2H<span class="indice">2</span>O</td> | ||
| + | <td>13.8</td> | ||
| + | <td>141.96</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>KH<span class="indice">2</span>PO<span class="indice">4</span></td> | ||
| + | <td>6.1</td> | ||
| + | <td>136.09</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>NaCl</td> | ||
| + | <td>1.0</td> | ||
| + | <td>58.44</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>NH<span class="indice">4</span>Cl</td> | ||
| + | <td>4.1</td> | ||
| + | <td>53.49</td> | ||
| + | </table> | ||
| + | |||
</section> | </section> | ||
Revision as of 20:08, 22 August 2012
Medium composition
LB medium
M9 medium
Add ingredients in the following order:| H2O | 396.2 mL |
| CaCl2 (1M) | 50 µL |
| MgSO4 (1M) | 1 mL |
| Salts (5X) | 100 mL |
| Vitamine B1 (1%) | 250 µL |
The composition for a 2X solution of the salts is the following:
| Amount for 1L in g | FW | |
|---|---|---|
| Na2HPO4 x 2H2O | 13.8 | 141.96 |
| KH2PO4 | 6.1 | 136.09 |
| NaCl | 1.0 | 58.44 |
| NH4Cl | 4.1 | 53.49 |
