"
Team:Grenoble/Biology/Protocols/Glycerol
From 2012.igem.org
(Difference between revisions)
(Created page with "{{:Team:Grenoble/Templates/Biology}} <html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en"> <body id="Biology"> <div id="cadre"> <section> <h1>Glycerol stock pr...") |
Greghansen (Talk | contribs) |
||
| (4 intermediate revisions not shown) | |||
| Line 7: | Line 7: | ||
<h1>Glycerol stock preparation</h1> | <h1>Glycerol stock preparation</h1> | ||
<h2>Goal</h2> | <h2>Goal</h2> | ||
| - | + | Glycerol stock is a way to store bacteria for a long period. | |
</section> | </section> | ||
<br/> | <br/> | ||
| Line 14: | Line 14: | ||
You need to: | You need to: | ||
<ol> | <ol> | ||
| - | <li> | + | <li>Grow a liquid culture overnight.</li> |
| - | <li> | + | <li>Prepare sterile 80% glycerol.</li> |
| - | <li> | + | <li>Mix 200 µL of glycerol and 800 µL of liquid culture.</li> |
</ol> | </ol> | ||
| + | <br/> | ||
Then store the glycerol stock at -80°C. | Then store the glycerol stock at -80°C. | ||
</section> | </section> | ||
Latest revision as of 08:53, 28 August 2012
Glycerol stock preparation
Goal
Glycerol stock is a way to store bacteria for a long period.Protocol
You need to:- Grow a liquid culture overnight.
- Prepare sterile 80% glycerol.
- Mix 200 µL of glycerol and 800 µL of liquid culture.
Then store the glycerol stock at -80°C.
