Team:Grenoble/Biology/Protocols/BB

===DNA Kit Plate Instructions=== To use the DNA in the Distribution Kit you may follow these instructions: :# With a pipette tip, punch a hole through the foil cover into the corresponding well of the Biobrick™-standard part that you want. [http://partsregistry.org/Help:Spring_2011_DNA_distribution#DNA_Kit_Plate_Orientation Make sure you have properly oriented the plate]. We recommend that you do not remove the foil cover, as it could lead to cross contamination between the wells. :# Pipette 10uL of dH2O (distilled water) into the well. Pipette up and down a few times and let sit for 5 minutes to make sure the dried DNA is fully resuspended. We recommend that you do not use TE to resuspend the dried DNA. :# [[Help:Transformation_Protocol|Transform]] 1 or 2uL of the resuspended DNA into your desired competent cells, plate your transformation with the appropriate antibiotic* and grow overnight. :#Pick a single colony and inoculate broth (again, with the correct antibiotic) and grow for 16 hours. :#Use the resulting culture to [http://openwetware.org/wiki/Miniprep/Kit-free_high-throughput_protocol miniprep] the DNA AND make your own glycerol stock (for further instruction on making a glycerol see [http://openwetware.org/wiki/Endy:Making_a_long_term_stock_of_bacteria this page]). We recommend using the miniprepped DNA to run QC tests, such as restriction digests and sequencing. ''* To know which antibiotics to use, look at the plasmid that the part is in. The [http://partsregistry.org/wiki/index.php/Help:Plasmids/Nomenclature naming scheme] for plasmids is specifically designed to indicate antibiotic resistance.'' ''Note: There is not enough DNA in each well to perform anything but [[Help:Transformation_Protocol|transformations]]''