Team:Grenoble/Biology/Notebook/July/week 33

From 2012.igem.org

Revision as of 10:50, 26 September 2012 by Adeline millet (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

iGEM Grenoble 2012

Project

August

a href="http://2012.igem.org/Team:Grenoble/Biology/Notebook/August">Week 31 • Week 32Week 33Week 34Week 35

Week 33: August 13th to 19th

Goal of the week:

Assembly of:
  • pAra/Bad_RBS_GFP and RBS_Cya.
  • pompC and mcherry
  • Tap and EnvZ

Monday, August 13th:

We did some verification PCRs on miniprep with HF Phusion enzyme (protocol) in order to check the Gibson Assembly products (12/09/09>.
Annealing temperature = 55°C.

To separate (protocol) the PCR products, we prepared a 1.8% TAE agarose gel.
Migration conditions = 100V during 30 min.
In order to reveal the DNA fragments, we used EtBr.

It showed no significant results (data not shown).

Tuesday, August 14th:

We did an experiment (protocol) in order to test the "AND" gate (CRP-cAMP & araC) without the amplifier module.

We transformed (new protocol) BW25113 WT competent cells (protocol) with pSB3C5.