Team:Grenoble/Biology/Notebook/July/week 30

From 2012.igem.org

Revision as of 13:48, 7 August 2012 by Adeline millet (Talk | contribs)

iGEM Grenoble 2012

iGEM Grenoble iGEM
iGEM 2012
main page
Project

July

week 27 week 28 week 29 week 30

Week 30: July 23rd to 29th

Goal of the week:

Test the Gibson Assembly and the transformation protocols, recover and amplify some biobricks involved in our genetic networks :
  • pAra/Bad_RBS_GFP (1300bp)
  • RBS_Cya (2600bp)
  • fha (80bp)
  • eCFP (800bp)
  • pSB4K5 (2400bp)

    • Monday, July 23rd:

    We did PCRs with HF Phusion enzyme (protocol) on miniprep in order to amplify pAra/Bad_RBS_GFP, and on colony to amplify RBS_Cya. We did the PCR both with and without DMSO.

    To separate the PCR products (12/07/23 and 12/07/20), we prepared a 1.8% TAE agarose gel.
    Migration conditions = 100V during 30 min.
    In order to reveal the DNA fragments, we used EtBr.

Retrieved from "http://2012.igem.org/Team:Grenoble/Biology/Notebook/July/week_30"