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Team:Grenoble/Biology/Notebook/July/week 30
From 2012.igem.org
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<li>pSB4K5 (2400bp)</li> | <li>pSB4K5 (2400bp)</li> | ||
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| - | <h2> | + | <h2> Monday, July 23<span class="exposant">rd</span>:</h2> |
| + | We did PCRs with HF Phusion enzyme (protocol) on miniprep in order to amplify pAra/Bad_RBS_GFP, and on colony to amplify RBS_Cya. We did the PCR both with and without DMSO.<br/> | ||
| + | <br/> | ||
| + | To separate the PCR products (12/07/23 and 12/07/20), we prepared a 1.8% TAE agarose gel.<br/> | ||
| + | Migration conditions = 100V during 30 min.<br/> | ||
| + | In order to reveal the DNA fragments, we used EtBr.<br/> | ||
| + | <br/> | ||
| + | |||
| + | |||
| + | |||
</section> | </section> | ||
</body> | </body> | ||
Revision as of 13:48, 7 August 2012
July
week 27 week 28 week 29 week 30Week 30: July 23rd to 29th
Goal of the week:
Test the Gibson Assembly and the transformation protocols, recover and amplify some biobricks involved in our genetic networks :- pAra/Bad_RBS_GFP (1300bp)
- RBS_Cya (2600bp)
- fha (80bp)
- eCFP (800bp)
- pSB4K5 (2400bp)
Monday, July 23rd:
We did PCRs with HF Phusion enzyme (protocol) on miniprep in order to amplify pAra/Bad_RBS_GFP, and on colony to amplify RBS_Cya. We did the PCR both with and without DMSO.To separate the PCR products (12/07/23 and 12/07/20), we prepared a 1.8% TAE agarose gel.
Migration conditions = 100V during 30 min.
In order to reveal the DNA fragments, we used EtBr.
