Team:Grenoble/Biology/Network

From 2012.igem.org

Revision as of 21:26, 23 September 2012 by Adeline millet (Talk | contribs)

iGEM Grenoble 2012

Project

Network details

Signaling module

The signaling module allows our bacterial strain to integrate the input signal = the pathogene presence.

Stapylococcus aureus secrete a protease nom de la protéase which cut a specific amino-acids sequence. This specific sequence can be used as a linker between a membrane protein and a dipeptide.
Once S. aureus is present, the linker is cut by the protease and the dipeptide is released.

The dipeptide bind to his receptor which is an engineered receptor:
  • the extracellular part is the extracellular part of Tap, a dipeptide receptor involved in the chemotaxism
  • the intracellular part is the intracellular part of EnvZ, an osmolarity sensor protein
Once the dipeptide is bound, the EnvZ part allows the phosphorylation of OmpR

Amplification module