"
Team:Grenoble/Biology/Network
From 2012.igem.org
(Difference between revisions)
| Line 23: | Line 23: | ||
Once <i>S. aureus</i> is present, the linker is cut by the protease and the dipeptide is released.<br/> | Once <i>S. aureus</i> is present, the linker is cut by the protease and the dipeptide is released.<br/> | ||
<br/> | <br/> | ||
| - | The dipeptide | + | The dipeptide binds to his receptor which is an engineered receptor: |
<ul><li>the extracellular part is the extracellular part of Tap, a dipeptide receptor involved in the chemotaxism</li> | <ul><li>the extracellular part is the extracellular part of Tap, a dipeptide receptor involved in the chemotaxism</li> | ||
<li>the intracellular part is the intracellular part of EnvZ, a kinase involved in the osmoregulation</li> | <li>the intracellular part is the intracellular part of EnvZ, a kinase involved in the osmoregulation</li> | ||
| Line 39: | Line 39: | ||
<br/> | <br/> | ||
<center><img src="https://static.igem.org/mediawiki/2012/c/c7/AND.png"/></center> | <center><img src="https://static.igem.org/mediawiki/2012/c/c7/AND.png"/></center> | ||
| - | + | <br/> | |
| + | cAMP binds to CRP (C-reactive protein) and then this complex activate the production of AraC. | ||
</section> | </section> | ||
</div> | </div> | ||
Revision as of 22:19, 23 September 2012
Network details
Our system is divided in two modules:- signaling module
- amplification module
Signaling module
The signaling module allows our bacterial strain to integrate the input signal = the pathogene presence.
Stapylococcus aureus secrete a protease nom de la protéase which cut a specific amino-acids sequence. This specific sequence can be used as a linker between a membrane protein and a dipeptide.
Once S. aureus is present, the linker is cut by the protease and the dipeptide is released.
The dipeptide binds to his receptor which is an engineered receptor:
- the extracellular part is the extracellular part of Tap, a dipeptide receptor involved in the chemotaxism
- the intracellular part is the intracellular part of EnvZ, a kinase involved in the osmoregulation
Once the dipeptide is bound, the EnvZ part allows the phosphorylation of OmpR, a transcriptional activator.
Amplification module
The amplification module allows our bacterial strain to amplify the input signal and to produce an output signal = fluorescence.
Once OmpR is phosphorylated, it allows the production of adenyl cyclase by activating the OmpC promoter.
Adenyl cyclase is an enzyme which catalyse the conversion of ATP (Adenosine Tri-Phosphate) to cAMP (cyclic Adenosine Mono-Phosphate).
cAMP binds to CRP (C-reactive protein) and then this complex activate the production of AraC.
