Team:Bordeaux/Tenth

From 2012.igem.org

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<li><a href="http://2012.igem.org/Team:Bordeaux/Eleventh">Eleventh Week</a></li>
<li><a href="http://2012.igem.org/Team:Bordeaux/Eleventh">Eleventh Week</a></li>
<li><a href="http://2012.igem.org/Team:Bordeaux/Twelfth">Twelfth Week</a></li>
<li><a href="http://2012.igem.org/Team:Bordeaux/Twelfth">Twelfth Week</a></li>
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<li><a href="http://2012.igem.org/Team:Bordeaux/Thirteenth">Thirteenth Week</a></li>
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Latest revision as of 18:49, 26 September 2012

Note Book - iGEM Bordeaux 2012

Day 44 : 03-09-2012


Today we did a miniprep of the precultures and runned a gel to test the plasmids.

Operon 2 completed !
But IVA5AIVB , 5AIIIC , IAIB ,ICIE and 5AIVDIVEIVC have to be redone.

Day 45 : 06-09-2012


Today we did the ligations for IVA5AIVB , 5AIVDIVEIVC and 5AIIIC
then inactivated the ligase after 1h 20 °C incubation and put overnight at 4°C

Day 46 : 07-09-2012


We transformed the ligations from yesterday plus biobricks IA ,IB ,IC ,IE and two new bricks IA' and IB' to make a new stock
We then put the plate at room temperature over the weekend