Search results

Create the page "Material and Methods" on this wiki!

• Team:TU Munich/Notebook/Labjournal
  P1-923 and PCR1-73 are the tube numbers for plasmids/PCR products from our [[Team:TU_M '''Hybridisation of the primers O1 with O2 and O3 with O4'''
  793 KB (114,680 words) - 14:38, 3 May 2013
• Team:Bielefeld-Germany/Labjournal
  ... formed of 15 students and weekly meetings began. Our team was established and it was time to find a suitable project. The first weekly meeting were more like big group brainstorming and we discussed idea, which in some cases were totally different from each oth
  333 KB (50,931 words) - 03:59, 27 October 2012
• Team:Potsdam Bioware/Lab/Labjournal/September
  ...olor: rgb(238, 221, 130); font-weight: bold;">Miniprep of mVENUS construct and wildtype AID from transfected CHO cells</p> *Miniprep of YFP-construct and wildtype AID from transfected CHO cells to proof whether it is possible to
  236 KB (34,819 words) - 22:05, 26 October 2012
• Jamboree/Team Abstracts
  ====[[Team:BostonU | Team BostonU]]: Abandon All Hope, Ye Who PCR: MoClo and the Quest for Genetic Circuit Characterization==== ...ies. We built and characterized various genetic circuits using MoClo Parts and compared them against their pre-existing BioBrick™ counterparts in order
  173 KB (25,944 words) - 17:38, 29 September 2013
• Team:XMU-China/wetlabjournal
  ...ion, how to run on a gel for restriction analysis, how to recover the DNA and ligate two target fragment.</p> We learn how to isolate the plasmids and how to run on a gel for restriction analysis. Everything is new for us!!!
  98 KB (16,065 words) - 19:36, 26 September 2012
• Team:Calgary/Notebook/Hydrocarbon
  ...e enclosed in <p> tags. For bulleted lists, <ul> tags will create the list and <li> tags will surround each list item. If there are any questions, please ...NO LONGER EXISTS AND WILL NOT APPEAR ON THE WIKI. Please migrate your data and information to your respective pages (as outlined in the email I sent you a
  101 KB (16,854 words) - 18:44, 21 September 2012
• Team:UC Davis/Notebook
  ...mmer. We rehydrated some parts from the distribution kits to get practice, and those parts were: J23101 E0240 E0040 R0010 pSB1C3 pSB1A3 pSB1AK3 pSB1K3 B00 ... or more commonly known as GFP! Our team took time to discuss our project, and comparing our interest with the research that has been conducted thus far,
  94 KB (14,677 words) - 03:40, 27 October 2012
• Team:Potsdam Bioware/Lab/Labjournal/August
  ...rgb(238, 221, 130); font-weight: bold;">glycerol stocks & miniprep of eGFP and GFP</p> preparative digestion with eGFP and CMV+mod. AID (without NES, with NLS)
  133 KB (20,216 words) - 22:44, 26 September 2012
• Team:TU Munich/Notebook/Meetings
  * Give detailed information, such as: nucleotide and/or amino acid sequence, Link to PDB-entry, ... ...f the system works unspecific, we thought that only general stress factors and transcription factors are involved. In this case, chances are that KlADH4 c
  103 KB (15,460 words) - 13:23, 26 September 2012
• Team:TU Darmstadt/Project/Philosophy
  ...="/Team:TU_Darmstadt/Project/Material_Science" title="Material Science">4. Material Science</a></li> The following technology assessment is the performance of Anja Pflug and Vanessa Parra Sanchez. It had a lasting effect on our opinion concerning ph
  57 KB (9,304 words) - 03:36, 26 September 2012
• Team:Calgary/Notebook/Desulfurization
  ...zation process as well as the reaction conditions we would want to employ, and identifying specific model compounds in addition to dibenzothiophene (DBT) ...S8 is the most studied model organism in investigations of the 4S pathway, and has been shown in many different research endeavors to be capable of conver
  65 KB (10,832 words) - 16:10, 3 September 2013
• Team:WHU-China/Project
  ...our probiotic-- E.coslim has three advantages: monitoring, high efficiency and long term efficiency.", "https://2012.igem.org/Team:WHU-China/Project?catal ...o novel synthetic promoters, Pcar and PfadR, our E.coslim is able to sense and respond to the high calorie molecules.", "https://2012.igem.org/Team:WHU-Ch
  91 KB (12,752 words) - 04:04, 27 October 2012
• Team:WHU-China/Notebooks
  ...our probiotic-- E.coslim has three advantages: monitoring, high efficiency and long term efficiency.", "https://2012.igem.org/Team:WHU-China/Project?catal ...o novel synthetic promoters, Pcar and PfadR, our E.coslim is able to sense and respond to the high calorie molecules.", "https://2012.igem.org/Team:WHU-Ch
  104 KB (15,269 words) - 04:04, 27 October 2012
• Team:Tec-Monterrey/antifreeze/notebook
  ...ng the course of this week we improved our dry lab research, sharing ideas and checking for possible mistakes."; apr_info[21] = "We worked on our projects and also we began to make budget estimates for the materials we would use in th
  69 KB (9,927 words) - 03:34, 26 October 2012
• Team:Tec-Monterrey/allergen/notebook
  ...ng the course of this week we improved our dry lab research, sharing ideas and checking for possible mistakes."; apr_info[21] = "We worked on our projects and also we began to make budget estimates for the materials we would use in th
  69 KB (9,925 words) - 03:29, 26 October 2012
• Team:Cornell/notebook/drylab/june
  ...Before the meeting was adjourned, we were assigned to research products or methods related to our components.<p></p> Dry Lab takes a close look at batteries and discusses various approaches to food delivery. <a href="#" class="technical
  43 KB (6,441 words) - 03:34, 27 October 2012
• Team:UNITN-Trento/Project
  ...ki needs it for you to enjoy the full experience. Please enable Javascript and refresh the page.</h2> ...reaction between calcite (i.e. CaCO3), the sulphur components of acid rain and atmospheric pollutants.--></p>
  64 KB (9,720 words) - 22:48, 26 October 2012
• Team:UNITN-Trento/Project/CrustAway
  ...ki needs it for you to enjoy the full experience. Please enable Javascript and refresh the page.</h2> ...reaction between calcite (i.e. CaCO3), the sulphur components of acid rain and atmospheric pollutants.--></p>
  64 KB (9,720 words) - 22:47, 26 October 2012
• Team:Evry/GB
  ... today contains almost over 3,000 basic characterized biological parts [2] and is a sources of inspiration for thousands of biologists around the world.<p ...mbling genetic parts together, leaving little time for characterizing them and testing their systems.</p>
  37 KB (6,031 words) - 00:29, 27 October 2012
• Team:Potsdam Bioware/Lab/Group Meetings
  presentation of topics, progress and deepening of projects<br> * knock-out mutant for magnetosomes – biosynthesis and transformation with essential gene
  48 KB (7,113 words) - 22:07, 26 October 2012
• Team:Wellesley HCI/Notebook/NicoleNotebook
  /*hide default igem banner and reformat style into blank slate*/ ...ssigned to research more about semantic search--what it was, its benefits, and whether or not it would be worthwhile to pursue this field in the context o
  43 KB (7,100 words) - 18:58, 3 October 2012
• Team:British Columbia/Human Practices/IP FAQ
  ... starting point for people trying to understand intellectual property laws and will guide you in determining the right direction for your project. The co ...aws vary from place to place. The content here is often a generalization, and will likely be lacking in jurisdiction-specific details.
  24 KB (3,914 words) - 03:55, 4 October 2012
• Team:Bielefeld-Germany/Protocols/molecular genetics
  = Bacterial specific methods = ...loning-and-dna-markers/cloning-tools-and-competent-cells/bacterial-strains-and-competent-cells/single-step-_krx_-competent-cells/ ''KRX''],
  30 KB (4,325 words) - 01:57, 27 September 2012
• Team:Cornell/project/drylab/components
  ...y for characterization. Finally, the device must be able to be sterilized and allow for sterile loading of Shewanella within the reactor at start-up. ...each end of the reactor were included to allow sterile loading, food feed, and electrodes.
  23 KB (3,617 words) - 03:35, 27 October 2012
• Team:Tianjin/Safety
  ...e our handbook can help people with the work of gene pollution prevention. And we believe our biosafety handbook will provide reference to other iGEM team ...he career of the gene pollution prevention, we will show the possibilities and examples in the third part.
  26 KB (3,898 words) - 03:57, 27 September 2012
• Team:MIT/MaterialsAndMethods
  document.title = "MIT iGEM - Methods"; <h1> Methods and Protocols Overview</h1>
  42 KB (6,746 words) - 03:12, 4 October 2012
• Team:TU Darmstadt/Project/Ecology
  ...="/Team:TU_Darmstadt/Project/Material_Science" title="Material Science">4. Material Science</a></li> ...ontent by Carolin Groß. Their evaluation is primarily focussed on Germany and Europe. This is due to the fact that there is hardly any detailed informati
  17 KB (2,633 words) - 01:23, 27 September 2012
• Team:Calgary/Notebook/Bioreactor
  ...e enclosed in <p> tags. For bulleted lists, <ul> tags will create the list and <li> tags will surround each list item. If there are any questions, please <p>Successfully completed both WHIMS and Biosafety level one training which provided us with training to ensure we a
  19 KB (3,352 words) - 03:27, 26 October 2012
• Team:Paris Bettencourt/Modeling
  ... in many fields of engineering, such as mechanical engineering, aircrafts, and manufactures. However, the risks they face are surely different from the ri *Differences in the physiology of natural and synthetic organisms will affect how they interact with the surrounding envi
  21 KB (3,033 words) - 02:47, 27 October 2012
• Team:Bielefeld-Germany/Sewage treatment plant
  ...Obere Lutter''] in Gütersloh with a population equivalent of over 300,000 and the treatment plant [http://www.schlossholtestukenbrock.de/060/sr_seiten/ar ...nds from the waste water. We were discussing about advantages, limitations and problems, that could arise.
  26 KB (3,703 words) - 02:01, 27 October 2012
• Team:Wellesley HCI/Notebook/NahumNotebook
  /*hide default igem banner and reformat style into blank slate*/ ... topics that the software developers were talking about. Being a biologist and a computer science lab wil be very interesting this summer…
  22 KB (3,750 words) - 18:57, 3 October 2012
• Team:HKUST-Hong Kong/Characterization
  ...a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Parts_and_Device">Parts and Devices</a></p></div> ...sing different methods. The results indicate that our parts are functional and we can quantitatively control their activities by changing the experimental
  50 KB (7,318 words) - 00:41, 27 September 2012
• Team:Calgary/Project/OSCAR/Bioreactor
  ...gn of OSCAR's home: culture conditions, method for hydrocarbon extraction, and containment of the genetically modified organisms. ...with such diverse applications as wastewater treatment, tissue engineering and beer fermentation.
  14 KB (2,311 words) - 02:51, 27 October 2012
• Team:NRP-UEA-Norwich/Human Outreach
  ...ting with all the other UK teams, in order to organize the UK team meet up and steam it live on the internet. ...he NRP-UEA team at Google campus London (Friday 17th August) The most open and publically accessible UK team meetup EVER===
  18 KB (2,902 words) - 00:52, 27 September 2012
• Team:UC Chile2/Bactomithril/Notebook
  ...and Bactomythril. The leaders of each group were elected: Carla (Cyanolux) and Claudia (Bactomithril). Carla and Max were chosen to maintain the wiki updated.
  23 KB (3,819 words) - 23:53, 21 September 2012
• Team:UC Chile/Bactomithril/notepad
  ...and Bactomythril. The leaders of each group were elected: Carla (Cyanolux) and Claudia (Bactomithril). Carla and Max were chosen to maintain the wiki updated.
  23 KB (3,784 words) - 02:45, 26 September 2012
• Team:TU Munich/Project/Safety
  ...rganisms (GMOs) in food production in general is relevant for this project and will be discussed here. ====General safety rules and safety briefing====
  14 KB (2,113 words) - 20:24, 26 October 2012
• Team:Colombia/Safety
  ... general regulations of the University regarding the proper standard rules and safety guidelines for laboratory practices. ...to. In our case, the Faculty of Sciences Department of Biological Sciences and Department of Physics.
  14 KB (2,161 words) - 00:17, 27 September 2012
• Team:Goettingen/News
  ...ately 3000 plastic petri dishes and let our <i>E. coli</i> strains grow on and in around 150 l LB-agar/broth. <br> Additionally all of us need new tires and brakes from driving up and down the “Faßberg” (the hill on which the MPI is positioned) by bike.
  22 KB (3,654 words) - 01:47, 27 September 2012
• Team:Leicester/Project
  ...e done some modeling of the first enzyme in the toluene degrading pathway, and future Leicester iGEM teams may be able to artificially mutate the first en ...le to degrade aromatic and aliphatic hydrocarbons that can fit polystyrene and its derivatives into their active sites. The bacteria that we insert these
  41 KB (6,336 words) - 02:27, 27 September 2012
• Team:Slovenia/SafetyMechanismsMicrocapsuleDegradation
  ... href='https://2012.igem.org/Team:Slovenia/ModelingMethods'><span>Modeling methods</span></a></li> ...><a href='https://2012.igem.org/Team:Slovenia/Notebook'><span>Experimental methods</span></a></li>
  40 KB (5,490 words) - 22:02, 26 October 2012
• Team:NTU-Taida/Human Practice/Safety-in-iGEM
  ...ng the interview, we demonstrated how iGEMers deal with biosafety problems and get feedbacks from the doctors.</p> ...teams. To monitor or to avoid spreading of BioBricks into environment, the methods showed even more diversity: adding a barcode or watermark that can be easil
  21 KB (3,264 words) - 19:42, 26 October 2012
• Team:Bielefeld-Germany/Protocols
  <li><a href="#5"><strong>Material</strong></a></li> ...tion of our protocol pages you can read more about our methods for cloning and BioBrick assembly.
  4 KB (636 words) - 11:39, 26 September 2012
• Team:Grenoble/Grenoble/Scientific
  ...r scientific center, especially in the fields of physics, computer science and applied mathematics. Grenoble’s high-tech expertise mainly revolves aroun ...issariat à l'Energie Atomique et aux énergies alternatives (CEA), INRIA, and other institutes.</p>
  15 KB (2,227 words) - 23:12, 25 September 2012
• Team:USTC-China/team
  alt="University of Science and Technology of China"/> ...artment of Chemistry, Duke University (USA) and Department of Biochemistry and Biophysics UNC-Chapel Hill (USA). Since 2001, he has been a professor of co
  28 KB (4,803 words) - 02:49, 27 September 2012
• Team:Tianjin/HumanPractice/Handbook
  ...e our handbook can help people with the work of gene pollution prevention. And we believe our biosafety handbook will provide reference to other iGEM team ...he career of the gene pollution prevention, we will show the possibilities and examples in the third part.
  13 KB (2,011 words) - 03:35, 27 September 2012
• Team:Bielefeld-Germany/Human Practices/Politics
  ...tag"). From left to right: Dr. Jörn Kalinowski, members of the two former and the actual iGEM Bielefeld teams, René Röspel.]] ...for realization, its advantages, risks and limits compared to conventional methods of waste water treatment.
  11 KB (1,678 words) - 02:53, 27 September 2012
• Team:Bielefeld-Germany/Protocols2
  <li><a href="#5"><strong>Material</strong></a></li> ...tion of our protocol pages you can read more about our methods for cloning and BioBrick assembly.
  4 KB (573 words) - 09:33, 20 September 2012
• Team:Exeter/Applications
  ...olymer itself. Polysaccharides appear in every corner of the natural world and have multiple applications ranging from protection to energy storage.</p> ... by doing so created a huge variety of uses within the medicinal, material and consumable sectors, as shown by the wealth of scientific literature availab
  19 KB (2,793 words) - 01:44, 27 September 2012
• Team:TU Darmstadt/Labjournal/Material Science
  ...="/Team:TU_Darmstadt/Project/Material_Science" title="Material Science">4. Material Science</a></li> = Material Science =
  19 KB (2,751 words) - 01:07, 27 September 2012
• Team:TU Darmstadt/Team
  ...="/Team:TU_Darmstadt/Project/Material_Science" title="Material Science">4. Material Science</a></li> .../Team:TU_Darmstadt/Results achievement] was made possible by the hard work and anticipation of the people of our team.</center>
  13 KB (2,161 words) - 03:38, 27 September 2012
• Team:Wageningen UR/Applications
  ... to construct ''standardized'' self-assembling particles with a ''simple'' and ''versatile'' [https://2012.igem.org/Team:Wageningen_UR/Coil_system attachm ...ps://2012.igem.org/Team:Wageningen_UR/Coil_system Plug ‘n Apply]) System and Virus-Like Particles ([https://2012.igem.org/Team:Wageningen_UR/VLPs VLPs])
  12 KB (1,790 words) - 16:14, 26 October 2012
• Team:Evry/Safety
  ...pped for bacterial genetics and molecular biology work in terms of devices and has <a href="http://www.who.int/csr/delibepidemics/WHO_CDS_CSR_LYO_2004_11/ ...udent working on site. All the iSSB research team is always around to help and advise us about good laboratory practices. </br></br>
  14 KB (2,193 words) - 21:45, 25 September 2012
• Team:Frankfurt/Safety
  ..., hands and eventually contaminated parts of the body are washed carefully and are disinfected with sterilium. ...thidiumbromide as a depleted solution for staining gels. The manufacturing and disposition of the ethidiumbromide solution is only done by experienced uni
  4 KB (580 words) - 17:29, 26 September 2012
• Team:Goettingen/testseite
  li:hover works in IE7 and FF2. a:hover works in IE6 and FF2.
  35 KB (5,138 words) - 08:47, 1 August 2012
• Team:Goettingen/Patrick
  li:hover works in IE7 and FF2. a:hover works in IE6 and FF2.
  35 KB (5,138 words) - 08:50, 1 August 2012
• Team:UC Chile2/SpiderColi/Notepad
  ...and Bactomythril. The leaders of each group were elected: Carla (Cyanolux) and Claudia (Bactomithril). Carla and Max were chosen to maintain the wiki updated.
  19 KB (2,992 words) - 22:58, 30 August 2012
• Team:UC Chile2/Bactomithril/Results
  ...and Bactomythril. The leaders of each group were elected: Carla (Cyanolux) and Claudia (Bactomithril). Carla and Max were chosen to maintain the wiki updated.
  19 KB (2,995 words) - 23:53, 21 September 2012
• Team:TU Darmstadt/Protocols/Metabolism
  ...="/Team:TU_Darmstadt/Project/Material_Science" title="Material Science">4. Material Science</a></li> * Inoculate 10 ml of LB-Media with an E. coli colony and incubate at 37 °C overnight
  17 KB (2,545 words) - 23:00, 26 September 2012
• Team:Washington/Optogenetics
  ...eppers.png|border|180px|right|thumb|An image of two peppers by shining red and green light on bacteria with light receptors.[3]]] ... wanted to make the tools to control optogenetic systems readily available and easier to access.
  18 KB (2,989 words) - 03:59, 4 October 2012
• Team:Leicester/July2012
  ..."open"], //Two CSS classes to be applied to the header when it's collapsed and expanded, respectively ["class1", "class2"] ..."none", "", ""], //Additional HTML added to the header when it's collapsed and expanded, respectively ["position", "html1", "html2"] (see docs)
  24 KB (4,216 words) - 01:14, 27 September 2012
• Team:Georgia Tech/HumanPractices
  ...in general and their research topic specifically by creating presentations and activities designed for the high school level. 1. Do an activity that will get the students up and moving. They sit behind a desk all day,You need to get them excited!
  15 KB (2,424 words) - 18:03, 3 October 2012
• Team:University College London/LabBook/Week11
  '''Methods:''' '''Results:''' The following table shows readings of the colony and halo diameters, plus the OD, taken at different times over 28 hours.
  9 KB (1,223 words) - 02:20, 27 September 2012
• Team:UT-Tokyo/Project/H2 E.coli/System
  == FHL complex and FhlA == ... hycBCDF, are thought to shuttle electrons between formate dehydrogenase H and Hyd-3.<br />
  9 KB (1,191 words) - 23:48, 26 September 2012
• Team:British Columbia/Team
  ... We have 2 undergraduate team members from the Faculty of Applied Sciences and 7 undergraduate team members from the Faculty of Science. ... engineering and environmental genomics, and my role in iGEM was designing and carrying out experiments to create a tunable synthetic consortium. I was al
  13 KB (1,939 words) - 02:35, 4 October 2012
• Team:Fudan Lux/nanotube
  ...for animal cells, which lack the rigid structure of the cell wall, to form and maintain such a plasmodesma-like structure? May it succeed, what changes ca ...lish a brand-new type of life form among Hela cells as a syncytial entity, and further understand the importance of this intimate communication. </p>
  24 KB (3,558 words) - 03:36, 27 September 2012
• Team:Goettingen/Project/Lab Work Flow
  * Note everything you have done the day and what you have prepared for the following day (e.g. inoculation of cultures) * Important methods can be marked with "Post-its".
  4 KB (578 words) - 21:04, 26 September 2012
• Team:USTC-China/background
  alt="University of Science and Technology of China"/> ...emperature and freeze. However, the phage is sensitive to ultraviolet rays and heat.</p>
  17 KB (2,616 words) - 23:31, 26 September 2012
• Team:ETH Zurich/MaterialMethods
  == Material & Methods == * Add 900 µL LB medium and incubate in a shaker at 37 °C 60 min.
  7 KB (988 words) - 02:49, 27 October 2012
• Team:NTNU Trondheim/Safety
  ...risk assessment (developed by the HSE group) was done of all the equipment and procedures to be used in this project. It had to be approved by the HSE gro ...(once a year, or less), 4 is high probability (once a month or more often) and 5 is very high probability (happens every week).
  10 KB (1,679 words) - 17:43, 25 September 2012
• Team:UT-Tokyo/LabWork/RegularMethods
  ...eader|fullpagename=Team:UT-Tokyo/LabWork/RegularMethod|subpagename=Regular Methods}} 2. Add 15uL of TE (MilliQ),and pipetting
  5 KB (681 words) - 00:09, 27 September 2012
• Team:Tec-Monterrey/antifreeze/safety
  ...ion of both the materials used in the projects, and the organisms employed and their characteristics.</p> <p>It is important to understand that we are always exposed to risk and no experiment is 100% risk free. Nevertheless, our job is to minimize the p
  30 KB (4,386 words) - 03:32, 26 October 2012
• Team:Tec-Monterrey/allergen/safety
  ...ion of both the materials used in the projects, and the organisms employed and their characteristics.</p> <p>It is important to understand that we are always exposed to risk and no experiment is 100% risk free. Nevertheless, our job is to minimize the p
  30 KB (4,384 words) - 03:26, 26 October 2012
• Team:Paris Bettencourt/Achievements
  ...r codon (stop codon) embedded in protein genes to prevent their expression and an amber suppressor system in our genetically engineered bacteria * Construction and characterization of 2 biobricks :
  18 KB (2,634 words) - 00:05, 27 October 2012
• Team:TU Darmstadt
  ...="/Team:TU_Darmstadt/Project/Material_Science" title="Material Science">4. Material Science</a></li> ...f as unprocessed waste in the environment. This often makes it into rivers and thus finally ends up in the world’s oceans.
  14 KB (2,191 words) - 03:47, 27 September 2012
• Team:Queens Canada/Guide/DNA
  <li> <a href="#Cloning_methods"> Cloning methods</a> </li> ... digesting with the insert with EcoRI, the insert will be cut prematurely, and its function will likely be reduced.
  15 KB (2,400 words) - 03:23, 27 October 2012
• Team:SUSTC-Shenzhen-B/protocol
  <p> 2. Restriction enzyme digestion and electrophoresis (proof)</p> <p> 8. Restriction Enzyme Digestion and Electrophoresis(proof) </p>
  15 KB (2,460 words) - 19:17, 24 September 2012
• Team:Goettingen/Sponsoring
  We are still looking for sponsors and supporters. If you would like to support as, contact us by [mailto:igem2012 ...ntains a broad range of varieties for sugar beet, corn, cereals, oil crops and potatoes. KWS conducted business
  8 KB (1,180 words) - 15:35, 24 September 2012
• Team:Exeter/Results
  ...m:Exeter/Results/characterise"; style="color:#57b947">Single Gene Plasmids and Enzyme Characterisation</a> ...rtant part of the iGEM project, acting as the mediator between the wet lab and dry lab teams. </p>
  14 KB (2,114 words) - 02:30, 27 September 2012
• Team:TU Darmstadt/Project/Material Science
  ...="/Team:TU_Darmstadt/Project/Material_Science" title="Material Science">4. Material Science</a></li> == Material Science ==
  6 KB (898 words) - 01:42, 27 September 2012
• Team:TU Darmstadt/Modeling MD
  ...="/Team:TU_Darmstadt/Project/Material_Science" title="Material Science">4. Material Science</a></li> ...efinement) force field which was especially developed to describe proteins and DNA.
  11 KB (1,668 words) - 01:06, 27 September 2012
• Team:Frankfurt/Ethics
  = Ethics and Synthetic Biology= ...thetic biology, we first need to define synthetic biology with its chances and risks.
  6 KB (1,028 words) - 04:07, 27 September 2012
• Team:UNITN-Trento/Art
  ...ki needs it for you to enjoy the full experience. Please enable Javascript and refresh the page.</h2> ...ur method and how it would have an impact on the art of stones restoration and conservation, we have taken steps to introduce experts in the field to our
  20 KB (3,095 words) - 12:41, 24 October 2012
• Team:SYSU-Software/Models
  ... FBA was applied in exploration of E.coli System[3,4,5].Thereafer, Palsson and his parters extend the uses of FBA even to genetic level[6,7].</p> ...tworks will reach homeostasis constrained by stoichiometry without knowing material changes all along the process. The values in this stoichiometric matrix are
  69 KB (12,645 words) - 00:58, 27 September 2012
• Team:Purdue/Biomaker Badge
  ...ation STEM: what girls have to say about science, technology, engineering, and mathematics </i>, the research concludes with the following ten recommendat ... Encourage young girls to ask questions about the world, to problem solve, and to use
  4 KB (596 words) - 03:57, 4 October 2012
• Team:Slovenia/Idea
  ... href='https://2012.igem.org/Team:Slovenia/ModelingMethods'><span>Modeling methods</span></a></li> ...><a href='https://2012.igem.org/Team:Slovenia/Notebook'><span>Experimental methods</span></a></li>
  29 KB (4,068 words) - 20:44, 26 October 2012
• Team:Slovenia/NotebookLabSafety
  ... href='https://2012.igem.org/Team:Slovenia/ModelingMethods'><span>Modeling methods</span></a></li> ...><a href='https://2012.igem.org/Team:Slovenia/Notebook'><span>Experimental methods</span></a></li>
  24 KB (3,285 words) - 20:46, 26 October 2012
• Team:HUST-China/Project/LCD/Method
  ...t, 9k-BBa_K805011 and ZXFα-bglX for cellulose degradation, 9k-BBa_K805012 and ZXFα-BBa_K805010 for hemicellulose degradation. ... as fusion protein with the C-terminal–half region of α-agglutinin Aga2 and β-xylosidase as fusion protein with the N-terminal-half region of ankyrin
  5 KB (695 words) - 01:49, 27 September 2012
• Team:Slovenia/SafetyMechanismsEscapeTag
  ... href='https://2012.igem.org/Team:Slovenia/ModelingMethods'><span>Modeling methods</span></a></li> ...><a href='https://2012.igem.org/Team:Slovenia/Notebook'><span>Experimental methods</span></a></li>
  26 KB (3,579 words) - 20:59, 26 October 2012
• Team:Calgary/Safety
  <h2>The Risks of FRED and OSCAR</h2> ...ons of horizontal gene transfer to native microorganisms in tailings ponds and the surrounding environment must be addressed. This issue has been raised b
  8 KB (1,309 words) - 21:08, 26 October 2012
• Team:Utah State/Safety
  ...roper materials, proper safety equipment (i.e., gloves, lab coats…etc.), and is conducted in a BSL 2 rated laboratory. ... being investigated in our lab and pose no threat to the public. All parts and devices are contained within vectors containing antibiotic resistance genes
  18 KB (2,388 words) - 05:49, 26 October 2012
• Team:LMU-Munich/Project Safety
  ...er to get a professional statement about the safety aspects of our project and how realistic it would be to release our spores in the environment, we invi In a two hour discussion, we explained the details of our projects and asked the Commissioner all of the "what ifs" on our minds. Most of the appl
  9 KB (1,500 words) - 14:18, 26 October 2012
• Team:Korea U Seoul/template/Notebook/Apr
  - Web design team (Wiki team) - Coding wiki pages and making video clips and flash movie. ...or to design most things except video clips and flash movie. Making poster and presentation files for the jamboree.
  12 KB (1,946 words) - 06:52, 15 August 2012
• Team:TU Darmstadt/Modeling Docking
  ...="/Team:TU_Darmstadt/Project/Material_Science" title="Material Science">4. Material Science</a></li> ...pounds like polymer plasticizer are released. Since PET is itself a waste material, it will never be in a pure state. Due to this, it is important to study po
  10 KB (1,666 words) - 20:29, 26 September 2012
• Team:British Columbia/Safety
  <b>A. Risks to the safety and health of team members or others in the lab?</b></br> ...red parts present a very low risk to the safety of the researcher, public, and the environment. Every team member has completed the introductory laborator
  8 KB (1,338 words) - 01:42, 4 October 2012
• Team:ULB-Brussels/Results
  ...href="https://2012.igem.org/Team:ULB-Brussels/Material&Methods">Material & Methods</a>&nbsp;/&nbsp; ...(MC1061), or containing a plasmid carrying the microcinB17 operon (McB17-3 and McB17-4) or containing a plasmid carrying the Mcc7 operon (Mcc7) were spott
  9 KB (1,357 words) - 00:24, 27 September 2012
• Team:Goettingen/NeuTest
  li:hover works in IE7 and FF2. a:hover works in IE6 and FF2.
  37 KB (4,985 words) - 18:58, 10 August 2012
• Team:Goettingen/NeuTest1
  li:hover works in IE7 and FF2. a:hover works in IE6 and FF2.
  37 KB (4,980 words) - 20:52, 10 August 2012
• Team:Goettingen/NeuTest2
  li:hover works in IE7 and FF2. a:hover works in IE6 and FF2.
  37 KB (4,985 words) - 20:53, 10 August 2012
• Team:Goettingen/NeuTest3
  li:hover works in IE7 and FF2. a:hover works in IE6 and FF2.
  37 KB (5,053 words) - 12:24, 12 August 2012
• Team:UC Chile/Biosafety
  ... conceived Two main systems, one is inspired by nature, easy to use, cheap and extensible to all gram negative bacteria that -if working properly- we urge To our vision a reality we propose the inclusion of a short and simple lysis gene under a constitutive promoter in all biobrick plasmids. T
  7 KB (1,144 words) - 03:25, 27 October 2012
• Team:Queens Canada/Safety
  ...safety equipment as well as properly disposing or cleaning of contaminated material. ...handling these substances, based on both the advice of our lab technicians and the MSDS.
  7 KB (1,031 words) - 04:07, 4 October 2012
• Team:NYU Gallatin/Project/Cloning
  ...enu-584"><a href="/Team:NYU_Gallatin/Notebook" title="Lab notebooks, news, and photos.">Notebook</a></li> ...d to the cellulose produced by Acetobacter xylinum using synthetic biology methods. Altering physical characteristics such as strength, color, odor, etc. woul
  18 KB (1,934 words) - 03:15, 4 October 2012
• Team:UC Chile2/Biosafety
  ... there may be environmental safety issues such as lateral DNA transference and endogenous species endangerment. We discuss this issue further in question ...ion by environmental cyanobacteria. To address this issue we have proposed and designed a biosafety mechanism discussed in question number 4 (see below).
  6 KB (857 words) - 00:07, 25 September 2012
• Team:UC Chile/Practices/Biosafety
  ... there may be environmental safety issues such as lateral DNA transference and endogenous species endangerment. We discuss this issue further in question ...ion by environmental cyanobacteria. To address this issue we have proposed and designed a biosafety mechanism discussed in question number 4 (see below).
  6 KB (851 words) - 00:32, 8 September 2012
• Team:Lyon-INSA/StateArt
  <h2>Mechanical and Chemical actions</h2> ...ons) and <strong>sanitizing</strong> (to kill attached surviving bacteria and viruses). Closed surfaces such as pipes, tanks or filters require specific
  9 KB (1,332 words) - 11:59, 8 June 2013
• Team:Edinburgh/Project/Non-antibiotic-Markers/Plac-RFP-SacB
  Alternative selectable and counter-selectable markers: ...010)</a>. This part was deposited into the Registry by Team Edinburgh 2010 and can be used as a counter selectable marker <a href="#bibliography" onclick
  14 KB (1,979 words) - 19:07, 26 October 2012
• Team:Nanjing China Bio/notbook6
  ...iments. He also presented the weakness of the anti-cancer drugs nowadays, and the orientation scientists are working towards, which made us have a deep u ...tions on specific bacterial targeting of tumors, intratumoral penetration, and native bacterial cytotoxicity. An later added illustrations on Salmonella
  12 KB (1,796 words) - 06:35, 25 September 2012
• Team:Nanjing China Bio/index/notbook6
  ...iments. He also presented the weakness of the anti-cancer drugs nowadays, and the orientation scientists are working towards, which made us have a deep u ...tions on specific bacterial targeting of tumors, intratumoral penetration, and native bacterial cytotoxicity. An later added illustrations on Salmonella
  12 KB (1,795 words) - 06:24, 25 September 2012
• Team:Nanjing China Bio/notebook6
  ...iments. He also presented the weakness of the anti-cancer drugs nowadays, and the orientation scientists are working towards, which made us have a deep u ...tions on specific bacterial targeting of tumors, intratumoral penetration, and native bacterial cytotoxicity. An later added illustrations on Salmonella
  12 KB (1,789 words) - 16:48, 26 September 2012
• Team:Bordeaux/Material
  ...="https://2012.igem.org/Team:Bordeaux/Material" class="current">Material & Methods</a></li> <h2>iGEM - Bordeaux - Material & methods</h2>
  7 KB (1,027 words) - 18:33, 26 September 2012
• Team:Peking/Project/Overview
  ...four other iGEM teams by sharing DNA materials, characterizing their parts and modeling! Click <a href="/Team:Peking/HumanPractice/Outreach/Collaboration ...sh iGEMers with a collection and praise of historic iGEM projects to share and learn from each other!Click <a href="/Team:Peking/HumanPractice/Review"><i>
  8 KB (1,233 words) - 07:48, 16 October 2012
• Team:TU Darmstadt/Supporters
  ...="/Team:TU_Darmstadt/Project/Material_Science" title="Material Science">4. Material Science</a></li> Special thanks go to the following people for their support and their contribution to our project.
  7 KB (1,029 words) - 02:05, 27 September 2012
• Team:ULB-Brussels/Introduction
  ...href="https://2012.igem.org/Team:ULB-Brussels/Material&Methods">Material & Methods</a>&nbsp;/&nbsp; ... is sometimes impossible to have a complete understanding of the reactions and mechanisms of different pathways.</p>
  12 KB (1,784 words) - 00:07, 27 September 2012
• Team:ULB-Brussels/Project
  ...href="https://2012.igem.org/Team:ULB-Brussels/Material&Methods">Material & Methods</a>&nbsp;/&nbsp; ... is sometimes impossible to have a complete understanding of the reactions and mechanisms of different pathways.</p>
  12 KB (1,785 words) - 00:07, 27 September 2012
• Team:Leicester/Chemistry
  ... as we all have many different skills that are of great use to find a safe and useful way to break down polystyrene.</p> ...g polymers into biodegradable polylactic acids. We took this idea on board and used it as a starting point for our research.</p>
  12 KB (1,969 words) - 21:48, 26 September 2012
• Team:Peking/HumanPractice/SowingTomorrowSyntheticBiologists/FormingAlliance
  .../42/Peking2012_Subsubmenu_HumanPractice_Sowing_Guidance.png" alt="Guidance and Funding Application" /> ...tion to the chief secretary and other teachers. Our efforts impressed them and finally we agreed to work together. The club also paved the way for our lec
  12 KB (1,608 words) - 16:11, 2 September 2012
• Team:Peking/HumanPractice/SowingTomorrowSyntheticBiologists/FormingAlliance old
  .../42/Peking2012_Subsubmenu_HumanPractice_Sowing_Guidance.png" alt="Guidance and Funding Application" /> ...tion to the chief secretary and other teachers. Our efforts impressed them and finally we agreed to work together. The club also paved the way for our lec
  12 KB (1,608 words) - 11:46, 16 September 2012
• Team:ZJU-China/labnote9.htm
  <h2>Week 9 bogged, Subversion and start again</h2> ...uates to manipulate MD, which could be replaced by RNA prediction software and ODE modeling with matlab.</p>
  9 KB (1,264 words) - 17:30, 26 October 2012
• Team:UNAM Genomics Mexico/Safety
  ...avoiding direct contact with hazardous reactants such as ethidium bromide, and the use of special protection for the eyes when observing stained gels with ...r animal disease. Furthermore, because of the scope of the project and the methods being used, our project would be classified as Biosecurity Level 1 accordin
  10 KB (1,509 words) - 00:54, 24 October 2012
• Team:Korea U Seoul/Notebook/Apr
  ...bacteria : Making bacteria detecting CO, H₂S, other toxic gases and compounds formed during incomplete combustion. After the detecting, noticea ...eria to maintain only cellular structures related to production of certain material we want.
  16 KB (2,073 words) - 02:11, 27 September 2012
• Team:ULB-Brussels/Material&Methods
  ...href="https://2012.igem.org/Team:ULB-Brussels/Material&Methods">Material & Methods</a>&nbsp;/&nbsp; <h1><A NAME="Material & Methods"> Material & Methods </A> </h1>
  17 KB (2,542 words) - 00:22, 27 September 2012
• Team:Peking/HumanPractice/Sowing/Forming
  ...tion to the chief secretary and other teachers. Our efforts impressed them and finally we agreed to work together. The club also paved the way for our lec ...ures presented the basic concept and research methods of synthetic biology and introduced the affairs of the iGEM high school division. How do we get in t
  6 KB (948 words) - 11:43, 26 October 2012
• Team:Bordeaux/Results
  <li><a href="https://2012.igem.org/Team:Bordeaux/Material" >Material & Methods</a></li> ...ning plan (fig.1). There were three steps to assemble properly each operon and one more step to pair the operons in order to have only two plasmids to ins
  8 KB (1,270 words) - 18:06, 26 September 2012
• Team:Goettingen/neu
  li:hover works in IE7 and FF2. a:hover works in IE6 and FF2.
  26 KB (3,537 words) - 18:15, 21 June 2012
• Team:ULB-Brussels/Materiel&Methods
  ...href="https://2012.igem.org/Team:ULB-Brussels/Material&Methods">Material & Methods</a>&nbsp;/&nbsp; <h1><A NAME="Material & Methods"> Material & Methods </A> </h1>
  13 KB (1,777 words) - 09:04, 25 September 2012
• Team:Hong Kong-CUHK/human1.html
  ...side. As for minimizing the environmental pollution, all laboratory wastes and bacteria stocks were autoclaved prior to disposal. </p> ...afety Office of the Chinese University of Hong Kong visited our laboratory and conducted a risk assessment.</p>
  7 KB (921 words) - 16:37, 7 September 2012
• Team:Hong Kong-CUHK/SAFETY QUES
  ...side. As for minimizing the environmental pollution, all laboratory wastes and bacteria stocks were autoclaved prior to disposal. </p> ...afety Office of the Chinese University of Hong Kong visited our laboratory and conducted a risk assessment.</p>
  4 KB (642 words) - 11:49, 26 September 2012
• Team:TU Darmstadt/Protocols/GC-MS/Results
  ...="/Team:TU_Darmstadt/Project/Material_Science" title="Material Science">4. Material Science</a></li> We used two [https://2012.igem.org/Team:TU_Darmstadt/Protocols/GC-MS methods] for our TPA analytics. Therefore we used
  8 KB (1,286 words) - 01:14, 27 September 2012
• Team:SYSU-Software/Models1
  ... FBA was applied in exploration of E.coli System[3,4,5].Thereafer, Palsson and his parters extend the uses of FBA even to genetic level[6,7].</p> ...tworks will reach homeostasis constrained by stoichiometry without knowing material changes all along the process. The values in this stoichiometric matrix are
  11 KB (1,598 words) - 07:11, 25 September 2012
• Team:Bordeaux/Introduction
  <li><a href="https://2012.igem.org/Team:Bordeaux/Material" >Material & Methods</a></li> ...plates in the lab. This fungus is growing in concentric circles on plates, and keep growing with this pattern if put on a new plate (figure 1).
  10 KB (1,405 words) - 18:33, 26 September 2012
• Team:Bordeaux/Biobricks
  <li><a href="https://2012.igem.org/Team:Bordeaux/Material" >Material & Methods</a></li> ... is a repressor of the pLsrA promoter; pAI-2 signal molecule binds to LsrR and inhibits its operation, therefore promoting transcription. LsrK protein (BB
  7 KB (1,067 words) - 18:38, 26 September 2012
• Team:TU Darmstadt/Protocols/Synthesis of polyethylene terephthalate in presence of sulfuric acid
  ...="/Team:TU_Darmstadt/Project/Material_Science" title="Material Science">4. Material Science</a></li> ...ethylene theephthalate is a reversible polykondensation of ethylene glycol and diemthyl terephthalate the developing methanol has to be removed from the r
  6 KB (833 words) - 21:24, 26 September 2012
• Team:Goettingen/Project/Lab Work Flow eng
  * Notice everything you have done the day and what you have prepared for the following day (e.g. inoculation of cultures) * Important methods can be marked with "Post-its"
  4 KB (572 words) - 21:04, 26 September 2012
• Team:HIT-Harbin/project/notebook/diary
  Create a flash showing the sprite of iGEM and the team of HIT.<br> Cartoon for spread of iGEM and synthesis biology around Harbin Institute of Technology.<br>
  10 KB (1,506 words) - 05:25, 23 October 2012
• Team:Cornell/project/background/oil sands
  ...eers to produce this resource on a large commercial scale in a responsible and sustainable way.
  3 KB (488 words) - 18:56, 28 May 2014
• Team:Cornell/testing/project/overview/1
  ...eers to produce this resource on a large commercial scale in a responsible and sustainable way.
  3 KB (420 words) - 16:01, 23 March 2016
• Team:Peking/Project/3D/3D
  ...such as with the synthesis of artificial vessels, organs, or bone tissues, and the creation of high-order biomaterials based on the high spatial resolutio ...ayer by layer. This is done by ‘cutting’ visual objects into 2D slides and printing each layer onto a surface.
  5 KB (720 words) - 05:14, 26 October 2012
• Team:TU Darmstadt/Project/Simulation
  ...="/Team:TU_Darmstadt/Project/Material_Science" title="Material Science">4. Material Science</a></li> ...e the evolution of single enzyme molecules by simulation of their dynamics and substrate specificity's.
  5 KB (763 words) - 01:38, 27 September 2012
• Team:Goettingen/G2 week3
  li:hover works in IE7 and FF2. a:hover works in IE6 and FF2.
  21 KB (2,744 words) - 11:30, 8 August 2012
• Agarose Gel Electrophoresis
  [[Category:Material and Methods]] ...ophoresis is the method of choice. Thereby, the DNA is separated by charge and size. The PCR samples are run on agarose gels with different percentages ac
  3 KB (529 words) - 09:19, 1 August 2012
• Team:Goettingen/G2 week4
  li:hover works in IE7 and FF2. a:hover works in IE6 and FF2.
  21 KB (2,758 words) - 11:46, 8 August 2012
• Team:Fudan Lux/project introduction
  ... This is the very first time that bacteria using light as an intercellular and intracellular signal in synthetic biology. The wave pattern or the synchron ...can’t be solved by simple computation, e.g. traffic jams between cities, and provide solutions to them.
  18 KB (2,447 words) - 03:57, 27 September 2012
• Team:Wageningen UR/Protocol
  = Methods = ...ew in iGEM. In this section we will explain how the different methods work and how it all fits together.
  3 KB (403 words) - 10:44, 26 October 2012
• Team:Goettingen/May03
  li:hover works in IE7 and FF2. a:hover works in IE6 and FF2.
  21 KB (2,760 words) - 19:42, 21 June 2012
• Team:Goettingen/May02
  li:hover works in IE7 and FF2. a:hover works in IE6 and FF2.
  22 KB (2,797 words) - 07:31, 8 August 2012
• Team:Goettingen/notebook
  li:hover works in IE7 and FF2. a:hover works in IE6 and FF2.
  22 KB (2,838 words) - 19:16, 21 June 2012
• Team:Goettingen/G2 week2
  li:hover works in IE7 and FF2. a:hover works in IE6 and FF2.
  21 KB (2,739 words) - 10:51, 8 August 2012
• Team:Goettingen/G2 week1
  li:hover works in IE7 and FF2. a:hover works in IE6 and FF2.
  21 KB (2,710 words) - 10:13, 8 August 2012
• Team:TU Darmstadt/Protocols/Bacterial transformation
  ...="/Team:TU_Darmstadt/Project/Material_Science" title="Material Science">4. Material Science</a></li> There a several methods of bacterial transformation, the process of inserting DNA into a host, the
  4 KB (520 words) - 18:41, 26 September 2012
• Team:Grenoble/Team/Students/Nadia
  Engineering studies in the field of Material science, 2<span class="exposant">nd</span> year ...u learn about other science fields and open your mind to different methods and different ways of thinking. So when I heard about the iGEM competition, I j
  2 KB (252 words) - 22:04, 26 September 2012
• Competent Cells
  [[Category:Material and Methods]] ...sure that the <chemform>CaCl2</chemform> buffer is ice-cold''' when needed and the centrifuge is cooled to 4°C
  1,012 B (149 words) - 09:21, 1 August 2012
• Cloning Protocols
  [[Category:Material and Methods]] ...ermoblock for 45 min (incubation time dependent on used vector!) at 37 °C and 300 rpm
  809 B (119 words) - 09:20, 1 August 2012
• Team:TU Darmstadt/Attributions
  ...="/Team:TU_Darmstadt/Project/Material_Science" title="Material Science">4. Material Science</a></li> ...pporters supporters] section, this includes inaugurations into measurement methods like MS, AFM or ELISA reader.
  3 KB (536 words) - 01:07, 27 September 2012
• Team:Bordeaux/Biology
  <li><a href="https://2012.igem.org/Team:Bordeaux/Material">Material & Methods</a></li> ...send a signal to its neighbor that will react to it by changing its color, and sending another message to the next cell. This will allow us to draw differ
  5 KB (783 words) - 18:52, 26 September 2012
• Standard PCR
  [[Category:Material and Methods]]
  250 B (37 words) - 09:16, 1 August 2012
• QuikChange Protocol
  [[Category:Material and Methods]]
  467 B (72 words) - 09:21, 1 August 2012
• Team:TU Darmstadt/Materials/Kanamycin
  ...="/Team:TU_Darmstadt/Project/Material_Science" title="Material Science">4. Material Science</a></li> # aliquot in 1 mL stocks and store at -20°C
  4 KB (530 words) - 20:42, 26 September 2012
• Team:USP-UNESP-Brazil/Plasmid Plug n Play/Experiments
  ...l/Templates/RImage | image=PlugPlaymethod8.png | caption=Material, Methods and participation in the project | size=620px}}
  247 B (26 words) - 22:30, 25 September 2012
• Team:TU Darmstadt/Protocols/PCR techniques
  ...="/Team:TU_Darmstadt/Project/Material_Science" title="Material Science">4. Material Science</a></li> ...asic polymerase chain reaction (PCR). Below is an overiew of important PCR methods with links to individual pages for detailed information.
  3 KB (504 words) - 21:21, 26 September 2012