Team:UNAM Genomics Mexico/Notebook/ANDSugar

From 2012.igem.org

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We made glycerols of the bacteria <br /> [[Team:UNAM_Genomics_Mexico/Notebook/Protocols#GLYCEROL_PROTOCOL | GLYCEROL PROTOCOL]] . <br />
We made glycerols of the bacteria <br /> [[Team:UNAM_Genomics_Mexico/Notebook/Protocols#GLYCEROL_PROTOCOL | GLYCEROL PROTOCOL]] . <br />
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We transformed plasmid pHp45 [[Team:UNAM_Genomics_Mexico/Notebook/Protocols#Transformation | TRANSFORMATION PROTOCOL]]. <br />
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We transformed plasmid pHp45 [[Team:UNAM_Genomics_Mexico/Notebook/Protocols#Transformation | TRANSFORMATION PROTOCOL]]. <br />
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<p class='captionInside'>1. 1 kb ladder <br />
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2.E1010
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We ran gel and extracted from gel [[Team:UNAM_Genomics_Mexico/Notebook/Protocols#Gel_extraction_protocol | LIQUID CULTURE]].<br />
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We made liquid cultures [[Team:UNAM_Genomics_Mexico/Notebook/Protocols#Liquid_culture | LIQUID CULTURE]] and lysed [LYSIS PROTOCOL]. <br />
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<h2>06/14/12</h2><br />
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Revision as of 04:44, 23 September 2012


UNAM-Genomics_Mexico


Contents

Arabinose/Xylose AND Gate



Nanotubes!! The logic Random info




  • 1. 1 kb ladder
    2.E1010



06/07/12


PY BROTH 10g salt/L
PSB2K3 kanamicin
We transformed RFP E1010.
plate 1 18F
plate 2 17E
Stock 50 mg/mL
E.coli 1/1000
TRANSFORMATION PROTOCOL.
We made liquid cultures with colonies LIQUID CULTURE.
We extracted plasmids PLASMID EXTRACTION PROTOCOL.
We ran a gel to check the extraction GEL ELECTROPHORESIS PROTOCOL.




06/12/12


We made glycerols of the bacteria
GLYCEROL PROTOCOL .
We transformed plasmid pHp45 TRANSFORMATION PROTOCOL.

  • 1. 1 kb ladder
    2.E1010



06/13/12


We ran gel and extracted from gel LIQUID CULTURE.
We made liquid cultures LIQUID CULTURE and lysed [LYSIS PROTOCOL].






06/14/12


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