Team:TMU-Tokyo/Notebook/Assay

From 2012.igem.org

(Difference between revisions)
 
(One intermediate revision not shown)
Line 31: Line 31:
<Br>
<Br>
<B>■Assay</B><Br>
<B>■Assay</B><Br>
 +
Assay0<Br>
Device1 Assay<Br>
Device1 Assay<Br>
Device2 Assay<Br>
Device2 Assay<Br>
Line 54: Line 55:
<p class="description">
<p class="description">
<b>■Assay0</b><Br>
<b>■Assay0</b><Br>
-
Resistant formaldehyde and formate→<A Href="https://2012.igem.org/Team:TMU-Tokyo/Notebook/Assay_0_Protocol_and_Result">Assay 0  Protocol and Result</A><Br><Br><Br></p>
+
Resistant formaldehyde and formate<Br>→<A Href="https://2012.igem.org/Team:TMU-Tokyo/Notebook/Assay_0_Protocol_and_Result">Assay 0  Protocol and Result</A><Br><Br><Br></p>
<p class="description">
<p class="description">
<b>■Assay1(Device1)</b><Br>
<b>■Assay1(Device1)</b><Br>
-
Detect formaldehyde →<A Href="https://2012.igem.org/Team:TMU-Tokyo/Notebook/Assay_1_Protocol_and_Result">Assay 1 Protocol and Result</A><Br><Br><Br></p>
+
Detect formaldehyde<Br>→<A Href="https://2012.igem.org/Team:TMU-Tokyo/Notebook/Assay_1_Protocol_and_Result">Assay 1 Protocol and Result</A><Br><Br><Br></p>
<p class="description">
<p class="description">
<b>■Assay2(Device2)</b><Br>
<b>■Assay2(Device2)</b><Br>

Latest revision as of 02:15, 27 September 2012

 




■Experiment
1st week (8.13 - 8.19)
2nd week (8.20 - 8.26)
3rd week (8.27 - 9. 2)
4th week (9. 3 - 9. 9)
5th week (9.10 - 9.16)
6th week (9.17 - 9.23)
7th week (9.24 - 9.30)


■Protocols


■Assay
Assay0
Device1 Assay
Device2 Assay
Device3 Assay




Assay



■Assay0
Resistant formaldehyde and formate
Assay 0 Protocol and Result


■Assay1(Device1)
Detect formaldehyde
Assay 1 Protocol and Result


■Assay2(Device2)
Resistant formaldehyde and Quantitative Analysis of Formaldehyde by Formaldehyde Test wako
Assay 2 Protocol and Result


■Assay3(Device3)
・Quantitative Analysis of formate by high performance liquid chromatography(HPLC)
・FDH4 requires NAD⁺ when formate dehydrogenate. So FDH activitiy Ant E. coli produces can be measured by reduction of NAD⁺ at 340nm.
Assay 3 Protocol and Result