Team:TMU-Tokyo/Notebook/Assay 1 Protocol and Result

■Experiment
1st week (8.13 - 8.19)
2nd week (8.20 - 8.26)
3rd week (8.27 - 9. 2)
4th week (9. 3 - 9. 9)
5th week (9.10 - 9.16)
6th week (9.17 - 9.23)
7th week (9.24 - 9.30)


■Protocols


■Assay
Assay0
Device1 Assay
Device2 Assay
Device3 Assay

Assay 1

■Assay1 Protocol

1. In LB medium (liquid), wound bacteria frm-GFP, were incubated for 6 hours at room shaking culture.
2. Was placed 5μl (0.04%) formalin solution to take the sample tube 500μl culture was diluted 200-fold there.
3.4 Degrees, I was centrifuged for 2 minutes at 120rpm
4. Supernatant was removed, I was vortexed
5. Put on the preparations taking 3μl from the tube, I observed a cover glass.
6. I observed as a control those that do not contain formaldehyde.

■Assay1 Result
GFP expression was not observed either. 50 times, 100 times, 400 times, 600 times, 800 times, created in 1000-fold diluted solution of the same procedure, but I was the same experiment, the expression of GFP was not observed either.