"
Team:TMU-Tokyo/Notebook/Assay
From 2012.igem.org
| (8 intermediate revisions not shown) | |||
| Line 31: | Line 31: | ||
<Br> | <Br> | ||
<B>■Assay</B><Br> | <B>■Assay</B><Br> | ||
| + | Assay0<Br> | ||
Device1 Assay<Br> | Device1 Assay<Br> | ||
Device2 Assay<Br> | Device2 Assay<Br> | ||
| Line 52: | Line 53: | ||
</p> | </p> | ||
<Br> | <Br> | ||
| + | <p class="description"> | ||
| + | <b>■Assay0</b><Br> | ||
| + | Resistant formaldehyde and formate<Br>→<A Href="https://2012.igem.org/Team:TMU-Tokyo/Notebook/Assay_0_Protocol_and_Result">Assay 0 Protocol and Result</A><Br><Br><Br></p> | ||
<p class="description"> | <p class="description"> | ||
<b>■Assay1(Device1)</b><Br> | <b>■Assay1(Device1)</b><Br> | ||
| - | + | Detect formaldehyde<Br>→<A Href="https://2012.igem.org/Team:TMU-Tokyo/Notebook/Assay_1_Protocol_and_Result">Assay 1 Protocol and Result</A><Br><Br><Br></p> | |
<p class="description"> | <p class="description"> | ||
<b>■Assay2(Device2)</b><Br> | <b>■Assay2(Device2)</b><Br> | ||
| - | Quantitative Analysis of Formaldehyde by<Br> | + | Resistant formaldehyde and Quantitative Analysis of Formaldehyde by Formaldehyde Test wako <Br> |
→<A Href="https://2012.igem.org/Team:TMU-Tokyo/Notebook/Assay_2_Protocol_and_Result">Assay 2 Protocol and Result</A><Br><Br></p> | →<A Href="https://2012.igem.org/Team:TMU-Tokyo/Notebook/Assay_2_Protocol_and_Result">Assay 2 Protocol and Result</A><Br><Br></p> | ||
| Line 65: | Line 69: | ||
<p class="description"> | <p class="description"> | ||
<b>■Assay3(Device3)</b><Br> | <b>■Assay3(Device3)</b><Br> | ||
| - | + | ・Quantitative Analysis of formate by high performance liquid chromatography(HPLC)<Br> | |
| + | ・FDH4 requires NAD⁺ when formate dehydrogenate. So FDH activitiy Ant E. coli produces can be measured by reduction of NAD⁺ | ||
at 340nm.<Br> | at 340nm.<Br> | ||
→<A Href="https://2012.igem.org/Team:TMU-Tokyo/Notebook/Assay_3_Protocol_and_Result">Assay 3 Protocol and Result</A><Br><Br> | →<A Href="https://2012.igem.org/Team:TMU-Tokyo/Notebook/Assay_3_Protocol_and_Result">Assay 3 Protocol and Result</A><Br><Br> | ||
| Line 76: | Line 81: | ||
<div class="clear"></div> | <div class="clear"></div> | ||
| + | <Br> | ||
</html> | </html> | ||
Latest revision as of 02:15, 27 September 2012
■Experiment
1st week (8.13 - 8.19)
2nd week (8.20 - 8.26)
3rd week (8.27 - 9. 2)
4th week (9. 3 - 9. 9)
5th week (9.10 - 9.16)
6th week (9.17 - 9.23)
7th week (9.24 - 9.30)
■Protocols
■Assay
Assay0
Device1 Assay
Device2 Assay
Device3 Assay
Assay
■Assay0
Resistant formaldehyde and formate
→Assay 0 Protocol and Result
■Assay1(Device1)
Detect formaldehyde
→Assay 1 Protocol and Result
■Assay2(Device2)
Resistant formaldehyde and Quantitative Analysis of Formaldehyde by Formaldehyde Test wako
→Assay 2 Protocol and Result
■Assay3(Device3)
・Quantitative Analysis of formate by high performance liquid chromatography(HPLC)
・FDH4 requires NAD⁺ when formate dehydrogenate. So FDH activitiy Ant E. coli produces can be measured by reduction of NAD⁺
at 340nm.
→Assay 3 Protocol and Result
