"
Team:METU/Timeline
From 2012.igem.org
Dumankolik (Talk | contribs) |
|||
| (11 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
<html lang="en-US" xmlns="http://www.w3.org/1999/xhtml" dir="ltr"> | <html lang="en-US" xmlns="http://www.w3.org/1999/xhtml" dir="ltr"> | ||
| - | + | <head> | |
| - | + | <meta http-equiv="Content-type" content="text/html; charset=utf-8" /> | |
| + | |||
<link rel="shortcut icon" href="http://simsekburak.com/hasan/css/images/favicon.ico" /> | <link rel="shortcut icon" href="http://simsekburak.com/hasan/css/images/favicon.ico" /> | ||
| - | + | <link rel="stylesheet" href="http://simsekburak.com/hasan/css/style.css" type="text/css" media="all" /> | |
| - | + | <!--[if IE 6]> | |
| - | + | <link rel="stylesheet" href="css/ie6.css" type="text/css" media="all" /> | |
| - | + | <script src="http://simsekburak.com/hasan/js/png-fix.js" type="text/javascript"></script> | |
| - | + | <![endif]--> | |
| - | + | <script src="http://simsekburak.com/hasan/js/jquery-1.4.2.js" type="text/javascript"></script> | |
| - | + | <script src="http://simsekburak.com/hasan/js/jquery.jcarousel.js" type="text/javascript"></script> | |
| - | + | <script src="http://simsekburak.com/hasan/js/js-func.js" type="text/javascript"></script> | |
| - | + | <style> | |
| - | + | h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{ | |
| - | + | display:none; | |
| - | + | } | |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| + | #top-section{ | ||
| + | height: 20px !important; | ||
| + | border: none !important; | ||
| + | } | ||
| - | + | div#content{ | |
| - | + | padding: 0 !important; | |
| - | + | background: transparent; | |
| - | + | border: none !important; | |
| + | width: auto; | ||
| + | } | ||
| + | |||
| + | </style> | ||
| + | |||
| + | |||
| + | </head> | ||
| + | <body style="background-image: url(https://static.igem.org/mediawiki/2012/c/cb/METU-team-logo-leaves.png); background-repeat: no-repeat; background-attachment: fixed; background-position:center;"> | ||
| + | |||
| + | |||
| + | <!-- Header --> | ||
<div id="header"> | <div id="header"> | ||
<div class="shell"> | <div class="shell"> | ||
| Line 47: | Line 52: | ||
<li><a href="https://2012.igem.org/Team:METU/Team"><span>Team</span></a></li> | <li><a href="https://2012.igem.org/Team:METU/Team"><span>Team</span></a></li> | ||
| - | <li><a href=" | + | <li><a href="#"><span>Project</span></a> |
<div class="dd-holder"> | <div class="dd-holder"> | ||
<div class="dd-t"></div> | <div class="dd-t"></div> | ||
<div class="dd"> | <div class="dd"> | ||
<ul> | <ul> | ||
| - | <li><a href="https://2012.igem.org/Team:METU/ | + | <li><a href="https://2012.igem.org/Team:METU/ProjectOverview"><span>Overview</span></a></li> |
| - | <li><a href="https://2012.igem.org/Team:METU/CODH"><span> | + | <li><a href="https://2012.igem.org/Team:METU/CODH"><span>CO Converter</span></a></li> |
<li><a href="https://2012.igem.org/Team:METU/KillSwitchOverview"><span>Kill-Switch</span></a></li> | <li><a href="https://2012.igem.org/Team:METU/KillSwitchOverview"><span>Kill-Switch</span></a></li> | ||
<li><a href="https://2012.igem.org/Team:METU/CellLimiter"><span>Cell Limiter</span></a></li> | <li><a href="https://2012.igem.org/Team:METU/CellLimiter"><span>Cell Limiter</span></a></li> | ||
| - | <li | + | <li><a href="https://2012.igem.org/Team:METU/datapage"><span>Data Page</span></a></li> |
</ul> | </ul> | ||
| Line 67: | Line 72: | ||
<li><a href="https://2012.igem.org/Team:METU/Results"><span>Results</span></a></li> | <li><a href="https://2012.igem.org/Team:METU/Results"><span>Results</span></a></li> | ||
| - | <li><a href=" | + | <li><a href="#"><span>Notebook</span></a> |
<div class="dd-holder"> | <div class="dd-holder"> | ||
<div class="dd-t"></div> | <div class="dd-t"></div> | ||
<div class="dd"> | <div class="dd"> | ||
<ul> | <ul> | ||
| - | + | ||
<li><a href="/Team:METU/Timeline">Timeline</a></li> | <li><a href="/Team:METU/Timeline">Timeline</a></li> | ||
| - | <li><a href="/Team:METU/Brainstorm"> | + | <li><a href="/Team:METU/Brainstorm">Brainstorming</a></li> |
<li><a href="https://2012.igem.org/Team:METU/Protocols">Protocols</a></li> | <li><a href="https://2012.igem.org/Team:METU/Protocols">Protocols</a></li> | ||
| - | + | ||
<li class="last"><a href="/Team:METU/Judging">Judging</a></li> | <li class="last"><a href="/Team:METU/Judging">Judging</a></li> | ||
</ul> | </ul> | ||
| Line 152: | Line 157: | ||
| | ||
<p><font face="tahoma" size="3" color="maroon"> | <p><font face="tahoma" size="3" color="maroon"> | ||
| - | <b>11.12.2011</b></font> :First Meeting | + | <b>11.12.2011</b></font> :First Meeting: |
Today the all that responded our announcements to be a part of the iGEM competition representing the METU-IGEM team got together for the first time. In total we have got 50 interested students, 2 instructors and 1 adviser. We started by introducing ourselves then we talked about our past IGEM experiences. Here, with the audiences we decided to make a brainstorming session which should be after reading enough about more on synthetic biology. Furthermore, we decided to make a brainstorming session to decide on our project after 1 week. | Today the all that responded our announcements to be a part of the iGEM competition representing the METU-IGEM team got together for the first time. In total we have got 50 interested students, 2 instructors and 1 adviser. We started by introducing ourselves then we talked about our past IGEM experiences. Here, with the audiences we decided to make a brainstorming session which should be after reading enough about more on synthetic biology. Furthermore, we decided to make a brainstorming session to decide on our project after 1 week. | ||
</p> | </p> | ||
| Line 158: | Line 163: | ||
<p><font face="tahoma" size="3" color="maroon"> | <p><font face="tahoma" size="3" color="maroon"> | ||
<b>18.12.2011</b></font>: Brainstorming Session Part 1: | <b>18.12.2011</b></font>: Brainstorming Session Part 1: | ||
| - | To make a successful plan we know that the first think should be done is coming together and adding the ideas each other. In the first part of the brainstorming we simply think everything and write them on the board! | + | To make a successful plan we know that the first think should be done is coming together and adding the ideas each other. In the first part of the brainstorming we simply think everything and write them on the board! |
</p> | </p> | ||
| Line 164: | Line 169: | ||
<p><font face="tahoma" size="3" color="maroon"> | <p><font face="tahoma" size="3" color="maroon"> | ||
<b>25.12.2011</b></font>: Brainstorming Session Part 2: | <b>25.12.2011</b></font>: Brainstorming Session Part 2: | ||
| - | In the second part of the brainstorming we continue to storm the ideas and writing down to the board. Then we made a schedule to think the ideas for their feasibility and if it is possible making circuits of the plans. | + | In the second part of the brainstorming we continue to storm the ideas and writing down to the board. Then we made a schedule to think the ideas for their feasibility and if it is possible making circuits of the plans. |
</p> | </p> | ||
| | ||
| Line 174: | Line 179: | ||
<p><font face="tahoma" size="3" color="maroon"> | <p><font face="tahoma" size="3" color="maroon"> | ||
<b>03.01.2012</b></font>: Eliminating the Ideas: | <b>03.01.2012</b></font>: Eliminating the Ideas: | ||
| - | The imagination of our team was extremely high because ,our team consists mainly first and second year students. At the end of the brainstorming sessions, there were lots of crazy ideas; however, we had to eliminate them. Finally we ended up with two main project. By trying to choose our main project, the several managerial tasks were divided among the team members. By doing so everyone got the chance to use their experience while learning from each other. Then we divided our team as a 4 different sub-groups; | + | The imagination of our team was extremely high because ,our team consists mainly first and second year students. At the end of the brainstorming sessions, there were lots of crazy ideas; however, we had to eliminate them. Finally we ended up with two main project. By trying to choose our main project, the several managerial tasks were divided among the team members. By doing so everyone got the chance to use their experience while learning from each other. Then we divided our team as a 4 different sub-groups; Wetlab workers, Software workers, People who interested in funding and Human Practice group.There were ofcourse some interactions between groups. |
</p> | </p> | ||
| Line 183: | Line 188: | ||
| | ||
<p><font face="tahoma" size="3" color="maroon"> | <p><font face="tahoma" size="3" color="maroon"> | ||
| - | <b>13.02.2012</b></font>: Article Reading | + | <b>13.02.2012</b></font>: Article Reading: |
| - | In the middle of the semester holiday, we did a meeting to give some | + | In the middle of the semester holiday, we did a meeting to give some homeworks to team members. We decided to prepare an article pool about our two main projects. By collecting information, we could have decided which one was more possible or which parts we had to add or remove from our projects. |
</p> | </p> | ||
<p><font face="tahoma" size="3" color="maroon"> | <p><font face="tahoma" size="3" color="maroon"> | ||
| - | <b>27.02.2012</b></font>: Deciding to main project | + | <b>27.02.2012</b></font>: Deciding to main project :CO FILTER must done! |
After semester holiday, we had read and summarized so many articles. With this huge amount of information we prepared lots of presentations to transfer the information and to discuss about them. After reading and discussing the so many articles related to our two main topics, we chose CO filter as our main project. | After semester holiday, we had read and summarized so many articles. With this huge amount of information we prepared lots of presentations to transfer the information and to discuss about them. After reading and discussing the so many articles related to our two main topics, we chose CO filter as our main project. | ||
</p> | </p> | ||
| Line 198: | Line 203: | ||
| | ||
<p><font face="tahoma" size="3" color="maroon"> | <p><font face="tahoma" size="3" color="maroon"> | ||
| - | <b>06.03.2012</b></font>: | + | <b>06.03.2012</b></font>: Building up the Project: |
| - | Because our team members were | + | Because our team members were unexperienced ,we had mini classes in which the basis of genetic engineering is taught. After this time, we all were excellent article readers. We tried to access all possible information about our project and try to build up the system, by finding available promoters ,genes,etc. We finally built the system and we presented it to our advisors. |
</p> | </p> | ||
<p><font face="tahoma" size="3" color="maroon"> | <p><font face="tahoma" size="3" color="maroon"> | ||
| - | <b>13.03.2012</b></font>: Searching for sponsorship | + | <b>13.03.2012</b></font>: Searching for sponsorship: |
Just after deciding our project, we started to search for sponsorship for our team. We started to make telephone calls to every single company or government unit about our project. In this time , we started to search for a new lab. | Just after deciding our project, we started to search for sponsorship for our team. We started to make telephone calls to every single company or government unit about our project. In this time , we started to search for a new lab. | ||
</p> | </p> | ||
<p><font face="tahoma" size="3" color="maroon"> | <p><font face="tahoma" size="3" color="maroon"> | ||
| - | <b>20.03.2012</b></font>: Building our own iGEM laboratory | + | <b>20.03.2012</b></font>: Building our own iGEM laboratory : |
| - | Our instructor ,Mrs. Akkaya had already offered her lab for our experiments. However, this wasn | + | Our instructor ,Mrs. Akkaya had already offered her lab for our experiments. However, this wasn`t enough for us. So after this search, we found a new lab in the basement of Chemistry Building. This lab was being used as storage for this building and was full of unnecessary stuff. By the time, we collected the last parts of our system and we all started to clean our new lab. |
</p> | </p> | ||
| Line 220: | Line 225: | ||
| | ||
<p><font face="tahoma" size="3" color="maroon"> | <p><font face="tahoma" size="3" color="maroon"> | ||
| - | <b>04.04.2012</b></font>: | + | <b>04.04.2012</b></font>: Software Meetings: |
Our software team started to do their own meetings. At first , they gather information about the model of our project. At the same time, they started to discussions about the design of our wiki page. By this time, they design a draft logo which is: (eski logo resmi) | Our software team started to do their own meetings. At first , they gather information about the model of our project. At the same time, they started to discussions about the design of our wiki page. By this time, they design a draft logo which is: (eski logo resmi) | ||
</p> | </p> | ||
<p><font face="tahoma" size="3" color="maroon"> | <p><font face="tahoma" size="3" color="maroon"> | ||
| - | <b>11.04.2012</b></font>: | + | <b>11.04.2012</b></font>: Human Practice Meetings: |
At this time, human practice sub team started to collect their ideas and present them to all group members. They made lots of presentation about their projects. Moreover, they implemented almost all of their ideas; making a short movie, organizing a iGEM party and a picnic with the other iGEM team of Turkey:Baskent University team,going to funfair to tell people about iGEM,etc. | At this time, human practice sub team started to collect their ideas and present them to all group members. They made lots of presentation about their projects. Moreover, they implemented almost all of their ideas; making a short movie, organizing a iGEM party and a picnic with the other iGEM team of Turkey:Baskent University team,going to funfair to tell people about iGEM,etc. | ||
</p> | </p> | ||
<p><font face="tahoma" size="3" color="maroon"> | <p><font face="tahoma" size="3" color="maroon"> | ||
| - | <b>18.04.2012</b></font>: Hardworking software team | + | <b>18.04.2012</b></font>: Hardworking software team: |
| - | Software team was arranging lots of meetings to talk about the design of our wiki page and the logo. After so many crazy ideas we ended with our current logo | + | Software team was arranging lots of meetings to talk about the design of our wiki page and the logo. After so many crazy ideas we ended with our current logo. After deciding our logo, software team started to ask the ideas of all team about wiki design. |
</p> | </p> | ||
<p><font face="tahoma" size="3" color="maroon"> | <p><font face="tahoma" size="3" color="maroon"> | ||
| - | <b>25.04.2012</b></font>: : Meetings with presidency of METU | + | <b>25.04.2012</b></font>: : Meetings with presidency of METU : |
| - | Our funding team arranged meetings with rector of our university. After those meetings, we found some money necessary for our wet lab practices. | + | Our funding team arranged meetings with the rector of our university. After those meetings, we found some money necessary for our wet lab practices. |
</p> | </p> | ||
| | ||
| Line 253: | Line 258: | ||
<p><font face="tahoma" size="3" color="maroon"> | <p><font face="tahoma" size="3" color="maroon"> | ||
| - | <b>09.05.2012</b></font>: | + | <b>09.05.2012</b></font>: Lets go into autoclave together!! |
We all introduced with autoclave at this times. All equipment that we had collected was autoclaved patiently and this procedure has been done often up to now. | We all introduced with autoclave at this times. All equipment that we had collected was autoclaved patiently and this procedure has been done often up to now. | ||
</p> | </p> | ||
| Line 267: | Line 272: | ||
| | ||
<p><font face="tahoma" size="3" color="maroon"> | <p><font face="tahoma" size="3" color="maroon"> | ||
| - | <b>06.06.2012</b></font>: Lab cleaning List | + | <b>06.06.2012</b></font>: Lab cleaning List done: |
| - | We all had started to live and work in this lab, so we have to keep it clean. We made a cleaning list and paste it on to our door. And we arrange a post stage for our lab notes | + | We all had started to live and work in this lab, so we have to keep it clean. We made a cleaning list and paste it on to our door. And we arrange a post stage for our lab notes, and as a compulsory work, we had to have a lab notebook to write down all procedures. |
</p> | </p> | ||
<p><font face="tahoma" size="3" color="maroon"> | <p><font face="tahoma" size="3" color="maroon"> | ||
| - | <b>13.06.2012</b></font>:Now Transformation List | + | <b>13.06.2012</b></font>:Now Transformation List: |
From iGEM kit we started to extract our parts and after diluting store them in fridges. Addition to this, we prepared competent cell series. Because we are very crowded wet lab team , we had to divide people into sub groups for transformation. | From iGEM kit we started to extract our parts and after diluting store them in fridges. Addition to this, we prepared competent cell series. Because we are very crowded wet lab team , we had to divide people into sub groups for transformation. | ||
</p> | </p> | ||
| Line 277: | Line 282: | ||
<p><font face="tahoma" size="3" color="maroon"> | <p><font face="tahoma" size="3" color="maroon"> | ||
<b>14.06.2012</b></font>: Protocols, have you seen my protocols? | <b>14.06.2012</b></font>: Protocols, have you seen my protocols? | ||
| - | The senior members of our team started to search for their reliable protocols. We collected all protocols that we could find. Then we copied all to our lab notebooks | + | The senior members of our team started to search for their reliable protocols. We collected all protocols that we could find. Then we copied all to our lab notebooks, and collected in a shared network. |
</p> | </p> | ||
| Line 307: | Line 312: | ||
<b>22.06.2012</b></font>: Meeting Time | <b>22.06.2012</b></font>: Meeting Time | ||
<ul> | <ul> | ||
| - | <li>Were these protocols true , why didn | + | <li>Were these protocols true , why didn`t they work, who are we :S</li> |
| - | <li>All transformations, except Berke`s , were failed. So we started to change our protocols and repeated all procedures with these new protocols. After that time, transformations started to work.</li> | + | <li>All transformations, except Berke`s , were failed. So we started to change our protocols and repeated all the procedures with these new protocols. After that time, transformations started to work.</li> |
<li>Funding team; | <li>Funding team; | ||
| - | They all started to telephone calls to companies. They found some companies who are ready to help us . And they arranged meetings with Güven Vakfi , Vendeka and | + | They all started to telephone calls to companies. They found some companies who are ready to help us . And they arranged meetings with Güven Vakfi , Vendeka and Atasim. Also they collect the information for application of TUBITAK.</li> |
</ul> | </ul> | ||
</p> | </p> | ||
<p><font face="tahoma" size="3" color="maroon"> | <p><font face="tahoma" size="3" color="maroon"> | ||
| - | <b>23.06.2012</b></font>: With new protocols transformations were started to repeat. | + | <b>23.06.2012</b></font>: With the new protocols transformations were started to repeat. |
</p> | </p> | ||
| Line 327: | Line 332: | ||
<p><font face="tahoma" size="3" color="maroon"> | <p><font face="tahoma" size="3" color="maroon"> | ||
| - | <b>26.06.2012</b></font> | + | <b>26.06.2012</b></font>: Our third group also succeeded the transformation of KS and RBS parts. |
</p> | </p> | ||
| Line 335: | Line 340: | ||
<p><font face="tahoma" size="3" color="maroon"> | <p><font face="tahoma" size="3" color="maroon"> | ||
| - | <b>28.06.2012</b></font>: | + | <b>28.06.2012</b></font>: The primers arrived and we dilute them according to their special value 1:10 ratio. We tested our primers with PCR. |
</p> | </p> | ||
<p><font face="tahoma" size="3" color="maroon"> | <p><font face="tahoma" size="3" color="maroon"> | ||
| - | <b>29.06.2012</b></font>: Meeting | + | <b>29.06.2012</b></font>: Meeting Time: |
<ul> | <ul> | ||
<li>Wetlab: | <li>Wetlab: | ||
| Line 359: | Line 364: | ||
<p><font face="tahoma" size="3" color="maroon"> | <p><font face="tahoma" size="3" color="maroon"> | ||
| - | <b>02.07.2012</b></font>: Colony PCR didn | + | <b>02.07.2012</b></font>: Colony PCR didn`t work for the first times. |
</p> | </p> | ||
<p><font face="tahoma" size="3" color="maroon"> | <p><font face="tahoma" size="3" color="maroon"> | ||
| - | <b>03.07.2012</b></font>: Colony PCR still didn | + | <b>03.07.2012</b></font>: Colony PCR still didn`t work. By the way we are trying to be success in transformations. Starting to cahenge the plan accordiging to tarnsformations works. |
</p> | </p> | ||
| Line 372: | Line 377: | ||
</p> | </p> | ||
<p><font face="tahoma" size="3" color="maroon"> | <p><font face="tahoma" size="3" color="maroon"> | ||
| - | <b>08.07.2012</b></font>: However, we realized that our gel electrophoresis has some problems. we couldn | + | <b>08.07.2012</b></font>: However, we realized that our gel electrophoresis has some problems. we couldn`t get any results. So we conclude that our ladder didn`t work. |
</p> | </p> | ||
<p><font face="tahoma" size="3" color="maroon"> | <p><font face="tahoma" size="3" color="maroon"> | ||
| Line 378: | Line 383: | ||
</p> | </p> | ||
<p><font face="tahoma" size="3" color="maroon"> | <p><font face="tahoma" size="3" color="maroon"> | ||
| - | <b>10.07.2012</b></font>: We control our | + | <b>10.07.2012</b></font>: We control our ONCs and we did plasmid isolation of some genes that work with transformation according to this protocol, and started to make stocks with them. After plasmid isolation we took nanodrop values of our plasmids. |
</p> | </p> | ||
<p><font face="tahoma" size="3" color="maroon"> | <p><font face="tahoma" size="3" color="maroon"> | ||
| Line 402: | Line 407: | ||
</p> | </p> | ||
<p><font face="tahoma" size="3" color="maroon"> | <p><font face="tahoma" size="3" color="maroon"> | ||
| - | <b>15.07.2012</b></font>: | + | <b>15.07.2012</b></font>: LB Broth ,LB agar and plates were prepared . |
</p> | </p> | ||
<p><font face="tahoma" size="3" color="maroon"> | <p><font face="tahoma" size="3" color="maroon"> | ||
| Line 412: | Line 417: | ||
</p> | </p> | ||
<p><font face="tahoma" size="3" color="maroon"> | <p><font face="tahoma" size="3" color="maroon"> | ||
| - | <b>18.07.2012</b></font>: | + | <b>18.07.2012</b></font>: Competent cells were done again. |
</p> | </p> | ||
<p><font face="tahoma" size="3" color="maroon"> | <p><font face="tahoma" size="3" color="maroon"> | ||
| - | <b>19.07.2012</b></font>: We had a picnic with Baskent iGEM team .We all had great time. | + | <b>19.07.2012</b></font>: We had a picnic with Baskent iGEM team .We all had great time and get fun together. |
</p> | </p> | ||
| Line 423: | Line 428: | ||
<p><font face="tahoma" size="3" color="maroon"> | <p><font face="tahoma" size="3" color="maroon"> | ||
| - | <b>21.07.2012</b></font>: Meeting | + | <b>21.07.2012</b></font>: Meeting: |
<ul> | <ul> | ||
<li>Preparation for iGEM party... | <li>Preparation for iGEM party... | ||
| Line 433: | Line 438: | ||
<p><font face="tahoma" size="3" color="maroon"> | <p><font face="tahoma" size="3" color="maroon"> | ||
| - | <b>22.07.2012</b></font>:We did ligation to our samples. | + | <b>22.07.2012</b></font>:We did ligation to our samples according the cloniing protocol. |
</p> | </p> | ||
<p><font face="tahoma" size="3" color="maroon"> | <p><font face="tahoma" size="3" color="maroon"> | ||
| Line 444: | Line 449: | ||
<b>25.07.2012</b></font>: Lab cleaning was done. | <b>25.07.2012</b></font>: Lab cleaning was done. | ||
</p> | </p> | ||
| - | + | ||
<p><font face="tahoma" size="3" color="maroon"> | <p><font face="tahoma" size="3" color="maroon"> | ||
| - | <b>28.07.2012</b></font>: Party Timee | + | <b>28.07.2012</b></font>: Party Timee for Synthetic biology lovers! |
| - | At that night, we had our party with 300 visitors. We had lots of surprises, which introduces iGEM, for our guests. We wore our lab coats and had great fun through all night. Up to early in the morning, the music wasn | + | At that night, we had our party with 300 visitors. We had lots of surprises, which introduces iGEM, for our guests. We wore our lab coats and had great fun through all night. Up to early in the morning, the music wasn`t closed. We left a great impression in our visitors?mind about iGEM competition. |
</p> | </p> | ||
| | ||
| Line 461: | Line 466: | ||
<b>03.08.2012</b></font>: Meeting: | <b>03.08.2012</b></font>: Meeting: | ||
<ul> | <ul> | ||
| - | <li>Software: </li> | + | <li>Software:Modelling works of KillSwitch and Cell limiter parts are divided into two in the Software team. We started to search up for parameters </li> |
| - | <li>Funding:</li> | + | <li>Funding:We started to go sponsorship meetings </li> |
</ul> | </ul> | ||
</p> | </p> | ||
| Line 481: | Line 486: | ||
<p><font face="tahoma" size="3" color="maroon"> | <p><font face="tahoma" size="3" color="maroon"> | ||
| - | <b>08.08.2012</b></font>: We controlled our parts , however ; we saw digestion hadn | + | <b>08.08.2012</b></font>: We controlled our parts , however ; we saw digestion hadn`t worked. |
</p> | </p> | ||
<p><font face="tahoma" size="3" color="maroon"> | <p><font face="tahoma" size="3" color="maroon"> | ||
| Line 529: | Line 534: | ||
</p> | </p> | ||
<p><font face="tahoma" size="3" color="maroon"> | <p><font face="tahoma" size="3" color="maroon"> | ||
| - | <b>22.08.2012</b></font>: Meeting | + | <b>22.08.2012</b></font>: Meeting Time: |
<ul> | <ul> | ||
| - | <li>Software: | + | <li>Software:We started to build up the wiki page. First of all we constructed general structure of it. </li> |
| - | + | <li>Wetlab: All wetlab workers gave feedbacks about the parts. </li> | |
| - | <li>Wetlab: </li> | + | |
</ul> | </ul> | ||
</p> | </p> | ||
| Line 543: | Line 547: | ||
</p> | </p> | ||
<p><font face="tahoma" size="3" color="maroon"> | <p><font face="tahoma" size="3" color="maroon"> | ||
| - | <b>25.08.2012</b></font>: | + | <b>25.08.2012</b></font>: These times since the sine rises a lot, some students go for a bit holiday, so we foced on more reading about articles. |
</p> | </p> | ||
<p><font face="tahoma" size="3" color="maroon"> | <p><font face="tahoma" size="3" color="maroon"> | ||
<b>26.08.2012</b></font>: We started to collect the articles in our dropbox for references. | <b>26.08.2012</b></font>: We started to collect the articles in our dropbox for references. | ||
| + | </p> | ||
| + | | ||
| + | | ||
| + | <p><font face="tahoma" size="6" color="maroon"> | ||
| + | <b>September,2012</b></font></p> | ||
| + | | ||
| + | </p> | ||
| + | <p><font face="tahoma" size="3" color="maroon"> | ||
| + | <b>06.09.2012</b></font>: To be sure about the parts that we tought they have done, we all run them in a gel. | ||
</p> | </p> | ||
<p><font face="tahoma" size="3" color="maroon"> | <p><font face="tahoma" size="3" color="maroon"> | ||
| - | <b> | + | <b>06.09.2012</b></font>: We scaned the procedures again and from this time we decided to use 3Assebly kits. |
</p> | </p> | ||
| - | + | <p><font face="tahoma" size="3" color="maroon"> | |
| + | <b>10.09.2012</b></font>: Plasmid isolation of the parts that have left one one before to ONC are done and run in the gel. | ||
| + | </p> | ||
| + | <p><font face="tahoma" size="3" color="maroon"> | ||
| + | <b>11.09.2012</b></font>: New competent cells are prepared by Selin and Özge. Elowitz and Double terminater parts are grown at ONC. Moreover, we made some transformations (Min C and the ligated part RBS+cI) | ||
| + | </p> | ||
| + | <p><font face="tahoma" size="3" color="maroon"> | ||
| + | <b>12.09.2012</b></font>: We made some restriction digestions. And the parts J23116 and Sender device are ligated. | ||
| + | </p> | ||
| + | <p><font face="tahoma" size="3" color="maroon"> | ||
| + | <b>13.09.2012</b></font>: Ligated parts are transformated. PCR checks are done also. Today is Emre`s birthday!! Happy b-day!! | ||
| + | </p> | ||
| + | <p><font face="tahoma" size="3" color="maroon"> | ||
| + | <b>14.09.2012</b></font>: Successful transformations were left for ONC. Ligation of the plasmids were done. | ||
| + | </p> | ||
| + | <p><font face="tahoma" size="3" color="maroon"> | ||
| + | <b>15.09.2012</b></font>: PCR of the parts were done. Then their gel control was done. Plasmid isolation of the parts are done. | ||
| + | </p> | ||
| + | <p><font face="tahoma" size="3" color="maroon"> | ||
| + | <b>16.09.2012</b></font>: Transformations of the ligated parts are done. Successful ones are left for ONC. | ||
| + | </p> | ||
| + | <p><font face="tahoma" size="3" color="maroon"> | ||
| + | <b>17.09.2012</b></font>: Morning, plasmid isolation of the parts were done, nonodrop measurements are handled and run in the gel. | ||
| + | </p> | ||
| + | <p><font face="tahoma" size="3" color="maroon"> | ||
| + | <b>19.09.2012</b></font>: Lab is cleaned, with team workers meetings are done. Wiki works getting more and more hard, nearly all files are uploaded into the IGEMs official side. | ||
| + | </p> | ||
| + | <p><font face="tahoma" size="3" color="maroon"> | ||
| + | <b>20.09.2012</b></font>: New competent cells are prepared. Furthermore, we decided to get together of the videos that we shoot just for fun. Oguz put the video together and while he was woking and hungering there, we forgot about him and ate some delicious pizza! Yeah! | ||
| + | </p> | ||
| + | </p> | ||
| + | <p><font face="tahoma" size="3" color="maroon"> | ||
| + | <b>21.09.2012</b></font>: We started to get very good feedbacks about our video. Moreover, some transformations are done. Ligations are done also. Hey, today is Oguz`s and Özge`s birtday! Let`s go out, ... Wait! We can`t. Too much stuff to do... ********!!!!! | ||
| + | </p> | ||
| + | </p> | ||
| + | <p><font face="tahoma" size="3" color="maroon"> | ||
| + | <b>22.09.2012</b></font>: Ligated parts were transformed. Yesterdays transformants made ONC. By the way, wiki works getting more and more hard. Wiki page however, nearly ready! | ||
| + | </p> | ||
| + | </p> | ||
| + | <p><font face="tahoma" size="3" color="maroon"> | ||
| + | <b>23.09.2012</b></font>: Plasmid isolation of the parts are done. Some ready parts had gone to PCR checking up. For this we again made Emre to preapare gel:) | ||
| + | </p> | ||
| + | </p> | ||
| + | <p><font face="tahoma" size="3" color="maroon"> | ||
| + | <b>24.09.2012</b></font>: Again, ligation of the parts were done. Transformations are prepared. ONC of the ready plates were prepared. From the parts are going it seems that the parts won`t be ready to submit :( One of the parts seems ready and we think characterization of CODH can be done at least. | ||
| + | </p> | ||
| + | </p> | ||
</div> | </div> | ||
| Line 565: | Line 624: | ||
<!-- End Main --> | <!-- End Main --> | ||
<!-- Footer --> | <!-- Footer --> | ||
| - | + | <div id="footer"> | |
| - | + | <div class="shell"> | |
| - | + | <p class="left">Copyright © 2010 RightDirection. All rights reserved. Designed by <a href="http://chocotemplates.com" target="_blank" title="The Sweetest CSS Templates WorldWide">Chocotemplates.com</a></p> | |
| - | + | <p class="right"> | |
| - | + | <a href="https://igem.org/Team.cgi?year=2012&team_name=METU">METU IGEM 2012</a> | |
| - | + | </p> | |
| - | + | <div class="cl"> </div> | |
| - | + | </div> | |
| - | + | </div> | |
| - | + | <!-- Footer --> | |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
</body> | </body> | ||
</html> | </html> | ||
Latest revision as of 22:46, 26 September 2012
December,2011
11.12.2011 :First Meeting: Today the all that responded our announcements to be a part of the iGEM competition representing the METU-IGEM team got together for the first time. In total we have got 50 interested students, 2 instructors and 1 adviser. We started by introducing ourselves then we talked about our past IGEM experiences. Here, with the audiences we decided to make a brainstorming session which should be after reading enough about more on synthetic biology. Furthermore, we decided to make a brainstorming session to decide on our project after 1 week.
18.12.2011: Brainstorming Session Part 1: To make a successful plan we know that the first think should be done is coming together and adding the ideas each other. In the first part of the brainstorming we simply think everything and write them on the board!
25.12.2011: Brainstorming Session Part 2: In the second part of the brainstorming we continue to storm the ideas and writing down to the board. Then we made a schedule to think the ideas for their feasibility and if it is possible making circuits of the plans.
January,2012
03.01.2012: Eliminating the Ideas: The imagination of our team was extremely high because ,our team consists mainly first and second year students. At the end of the brainstorming sessions, there were lots of crazy ideas; however, we had to eliminate them. Finally we ended up with two main project. By trying to choose our main project, the several managerial tasks were divided among the team members. By doing so everyone got the chance to use their experience while learning from each other. Then we divided our team as a 4 different sub-groups; Wetlab workers, Software workers, People who interested in funding and Human Practice group.There were ofcourse some interactions between groups.
February,2012
13.02.2012: Article Reading: In the middle of the semester holiday, we did a meeting to give some homeworks to team members. We decided to prepare an article pool about our two main projects. By collecting information, we could have decided which one was more possible or which parts we had to add or remove from our projects.
27.02.2012: Deciding to main project :CO FILTER must done! After semester holiday, we had read and summarized so many articles. With this huge amount of information we prepared lots of presentations to transfer the information and to discuss about them. After reading and discussing the so many articles related to our two main topics, we chose CO filter as our main project.
March,2012
06.03.2012: Building up the Project: Because our team members were unexperienced ,we had mini classes in which the basis of genetic engineering is taught. After this time, we all were excellent article readers. We tried to access all possible information about our project and try to build up the system, by finding available promoters ,genes,etc. We finally built the system and we presented it to our advisors.
13.03.2012: Searching for sponsorship: Just after deciding our project, we started to search for sponsorship for our team. We started to make telephone calls to every single company or government unit about our project. In this time , we started to search for a new lab.
20.03.2012: Building our own iGEM laboratory : Our instructor ,Mrs. Akkaya had already offered her lab for our experiments. However, this wasn`t enough for us. So after this search, we found a new lab in the basement of Chemistry Building. This lab was being used as storage for this building and was full of unnecessary stuff. By the time, we collected the last parts of our system and we all started to clean our new lab.
April,2012
04.04.2012: Software Meetings: Our software team started to do their own meetings. At first , they gather information about the model of our project. At the same time, they started to discussions about the design of our wiki page. By this time, they design a draft logo which is: (eski logo resmi)
11.04.2012: Human Practice Meetings: At this time, human practice sub team started to collect their ideas and present them to all group members. They made lots of presentation about their projects. Moreover, they implemented almost all of their ideas; making a short movie, organizing a iGEM party and a picnic with the other iGEM team of Turkey:Baskent University team,going to funfair to tell people about iGEM,etc.
18.04.2012: Hardworking software team: Software team was arranging lots of meetings to talk about the design of our wiki page and the logo. After so many crazy ideas we ended with our current logo. After deciding our logo, software team started to ask the ideas of all team about wiki design.
25.04.2012: : Meetings with presidency of METU : Our funding team arranged meetings with the rector of our university. After those meetings, we found some money necessary for our wet lab practices.
May,2012
02.05.2012:Readdyyy for wet lab!! After cleaning, we designed our laboratory .Because our lab was a radioactive working place, we had to remove lots of stuff and check whole lab for radioactive substances. Because of our limited budget ,we can only place an old PCR machine named as UMUT. Then we had opened the box sent by iGEM with big excitement for the first time.
09.05.2012: Lets go into autoclave together!! We all introduced with autoclave at this times. All equipment that we had collected was autoclaved patiently and this procedure has been done often up to now.
23.05.2012: Preparing plates.. As a beginning of our wet lab work, we prepared lots of plates with agar medium. It was necessary to prepare them continuously during our working period. The first preparation was so excited for every one of us; however after time passing, this part has become the most boring part of study.
June,2012
06.06.2012: Lab cleaning List done: We all had started to live and work in this lab, so we have to keep it clean. We made a cleaning list and paste it on to our door. And we arrange a post stage for our lab notes, and as a compulsory work, we had to have a lab notebook to write down all procedures.
13.06.2012:Now Transformation List: From iGEM kit we started to extract our parts and after diluting store them in fridges. Addition to this, we prepared competent cell series. Because we are very crowded wet lab team , we had to divide people into sub groups for transformation.
14.06.2012: Protocols, have you seen my protocols? The senior members of our team started to search for their reliable protocols. We collected all protocols that we could find. Then we copied all to our lab notebooks, and collected in a shared network.
15.06.2012: Competent cells were prepared according to our protocol. A new list was done to do competent cell.
16.06.2012:Let the transformations begin! All the members of our team started to learn how to do transformation. All parts, removed and diluted, were ready to do transformation. There were almost ten groups for doing transformation. All started to their work one by one.
18.06.2012:We did the transformation of PLasR and JD.
19.06.2012: Transformation of CI and Elowitz were done by second group.
20.06.2012: KS and RBS were done ,too.
21.06.2012: Plates were cleaned and the contaminated LB was poured.
22.06.2012: Meeting Time
- Were these protocols true , why didn`t they work, who are we :S
- All transformations, except Berke`s , were failed. So we started to change our protocols and repeated all the procedures with these new protocols. After that time, transformations started to work.
- Funding team; They all started to telephone calls to companies. They found some companies who are ready to help us . And they arranged meetings with Güven Vakfi , Vendeka and Atasim. Also they collect the information for application of TUBITAK.
23.06.2012: With the new protocols transformations were started to repeat.
24.06.2012: With our new protocol , the transformation of PLasR and JD were succeed.
25.06.2012: The parts,CI and Elowitz, were also done .
26.06.2012: Our third group also succeeded the transformation of KS and RBS parts.
27.06.2012:Our last group did the transformation of LacI ,LasR and J23116. They also did LB agar with ampicillin according to LB agar protocol.
28.06.2012: The primers arrived and we dilute them according to their special value 1:10 ratio. We tested our primers with PCR.
29.06.2012: Meeting Time:
- Wetlab: Protocols were being collected for next procedures.
- Software: Software team started to build the model of kill switch and quorum sensing. They needed some parameters for these models .So lots of homework was given to all team to do some research about them.
- Funding: Our rectorship started to give some funding for our wet lab procedures. Then they started to search funding for our plane tickets and accommodation.
30.06.2012: We did the colony PCR from plates which we cultivate. The iGEM team of Baskent University came to our university for meeting .We decided for collaboration.
July,2012
02.07.2012: Colony PCR didn`t work for the first times.
03.07.2012: Colony PCR still didn`t work. By the way we are trying to be success in transformations. Starting to cahenge the plan accordiging to tarnsformations works.
04.07.2012: By changing some conditions we tried colony pcr several times ,to find the error.
07.07.2012:After some tries ,colony PCR was succeed and run samples on agorose gel according to its protocol. According to results, we put ONC into the shaker.
08.07.2012: However, we realized that our gel electrophoresis has some problems. we couldn`t get any results. So we conclude that our ladder didn`t work.
09.07.2012: We tried to find new ladder from our colleagues.
10.07.2012: We control our ONCs and we did plasmid isolation of some genes that work with transformation according to this protocol, and started to make stocks with them. After plasmid isolation we took nanodrop values of our plasmids.
11.07.2012: We ran the plasmids which we isolated in agorose gel electrophoresis. We prepared LB Broth, LB Agar and plates.
12.07.2012: We prepared antibiotics ampicillin and kanamycin according to protocol1 and protocol2.
13.07.2012: We cleaned up our lab.
14.07.2012: Meeting
- Wetlab: Protocols were being collected for next procedures.
- Software: Software team started to build the model of kill switch and quorum sensing. They needed some parameters for these models .So lots of homework was given to all team to do some research about them.
- Funding: Our rectorship started to give some funding for our wet lab procedures. Then they started to search funding for our plane tickets and accommodation.
15.07.2012: LB Broth ,LB agar and plates were prepared .
16.07.2012: Funding team went to Interlab for sponsorship discussion.
17.07.2012: We did double digestion of our plasmids according to its protocol. Then put the samples in Agorose Gel Electrophoresis and we did gel extraction according to its protocol.
18.07.2012: Competent cells were done again.
19.07.2012: We had a picnic with Baskent iGEM team .We all had great time and get fun together.
20.07.2012: We had a mini meeting for logo ideas. And we decided some details , so it was ready to professionally drawn.
21.07.2012: Meeting:
- Preparation for iGEM party... With the wet lab works, we also carried out our human practice works. And we found a place for our party. And we designed our party tickets and party posters.(resimler) .We started to deliver our party flyers.
- Software: After collecting homework, software team did some parts of models and parameters. They also started to talk about logo design.
- Human Practice: They started to talk about the activities which were going to do in METU.
22.07.2012:We did ligation to our samples according the cloniing protocol.
23.07.2012: We controlled our samples with Agorose Gel electrophoresis. We did transformation from our ligated parts and spread them onto agar plate and we did ONC.
24.07.2012:Preparation for party is done and last controls were done.
25.07.2012: Lab cleaning was done.
28.07.2012: Party Timee for Synthetic biology lovers! At that night, we had our party with 300 visitors. We had lots of surprises, which introduces iGEM, for our guests. We wore our lab coats and had great fun through all night. Up to early in the morning, the music wasn`t closed. We left a great impression in our visitors?mind about iGEM competition.
August,2012
02.08.2012: We did plasmid isolation optimization in 4 test isolation condition according to its protocol.
03.08.2012: Meeting:
- Software:Modelling works of KillSwitch and Cell limiter parts are divided into two in the Software team. We started to search up for parameters
- Funding:We started to go sponsorship meetings
04.08.2012: We did agorose gel electrophoresis and measure the nanodrop of the parts.
05.08.2012: We did ONC from samples.
06.08.2012: We did plasmid isolation and measure their nanodrop , and put samples into agorose gel electrophoresis.
07.08.2012: We did double digestion .
08.08.2012: We controlled our parts , however ; we saw digestion hadn`t worked.
09.08.2012: We repeated our digestion procedure according to its protocol.
10.08.2012: Our final logo was drawn and we loved it.
11.08.2012: We did ligation and gel electrophoresis.
12.08.2012: We did transformation of ligated parts, and realized some of the ligations were not successfully done.
13.08.2012: The unsuccessful ligations were repeated.
14.08.2012:The last ligations were also be controlled by transformation.
15.08.2012: We prepared competent cell with CaCl2 treatment.
16.08.2012: Meeting
- Wetlab: We tried to collect all the equipment that we used to write them down to sponsorship list.
- Software: Models were nearly completed and wiki codes were also nearly completed.
- Funding: Our funding team said us that our plane tickets were bought?
17.08.2012: We did plasmid isolation for the parts; pLacI, KS, SD, J23116,pLasR and measure their nanodrop. We did competent cells and digestion.
18.08.2012: We did transformations of RBS,CI,LVA and minC parts.
19.08.2012: We did gel electrophoresis and we saw the DT-1 and pLacI.
21.08.2012: We did gel extraction.
22.08.2012: Meeting Time:
- Software:We started to build up the wiki page. First of all we constructed general structure of it.
- Wetlab: All wetlab workers gave feedbacks about the parts.
23.08.2012: We did competent cells.
24.08.2012: We talked about our wiki page and its design. We added some new parts to our wiki draft.
25.08.2012: These times since the sine rises a lot, some students go for a bit holiday, so we foced on more reading about articles.
26.08.2012: We started to collect the articles in our dropbox for references.
September,2012
06.09.2012: To be sure about the parts that we tought they have done, we all run them in a gel.
06.09.2012: We scaned the procedures again and from this time we decided to use 3Assebly kits.
10.09.2012: Plasmid isolation of the parts that have left one one before to ONC are done and run in the gel.
11.09.2012: New competent cells are prepared by Selin and Özge. Elowitz and Double terminater parts are grown at ONC. Moreover, we made some transformations (Min C and the ligated part RBS+cI)
12.09.2012: We made some restriction digestions. And the parts J23116 and Sender device are ligated.
13.09.2012: Ligated parts are transformated. PCR checks are done also. Today is Emre`s birthday!! Happy b-day!!
14.09.2012: Successful transformations were left for ONC. Ligation of the plasmids were done.
15.09.2012: PCR of the parts were done. Then their gel control was done. Plasmid isolation of the parts are done.
16.09.2012: Transformations of the ligated parts are done. Successful ones are left for ONC.
17.09.2012: Morning, plasmid isolation of the parts were done, nonodrop measurements are handled and run in the gel.
19.09.2012: Lab is cleaned, with team workers meetings are done. Wiki works getting more and more hard, nearly all files are uploaded into the IGEMs official side.
20.09.2012: New competent cells are prepared. Furthermore, we decided to get together of the videos that we shoot just for fun. Oguz put the video together and while he was woking and hungering there, we forgot about him and ate some delicious pizza! Yeah!
21.09.2012: We started to get very good feedbacks about our video. Moreover, some transformations are done. Ligations are done also. Hey, today is Oguz`s and Özge`s birtday! Let`s go out, ... Wait! We can`t. Too much stuff to do... ********!!!!!
22.09.2012: Ligated parts were transformed. Yesterdays transformants made ONC. By the way, wiki works getting more and more hard. Wiki page however, nearly ready!
23.09.2012: Plasmid isolation of the parts are done. Some ready parts had gone to PCR checking up. For this we again made Emre to preapare gel:)
24.09.2012: Again, ligation of the parts were done. Transformations are prepared. ONC of the ready plates were prepared. From the parts are going it seems that the parts won`t be ready to submit :( One of the parts seems ready and we think characterization of CODH can be done at least.
