Team:Groningen/description

From 2012.igem.org

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<z1 >Project</z1><br>
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<z1 >Abstract</z1><br>
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<div class="Death2">
<div class="Death2">
<div class="Death1">
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<DeathP ><A HREF="https://2012.igem.org/Team:Groningen/Death" TARGET="_blank"><FONT COLOR=#ffffff>death</FONT></A></DeathP>
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<DeathP ><A HREF="https://2012.igem.org/Team:Groningen/Death" TARGET="_blank"><FONT COLOR=#ffffff>kill switch</FONT></A></DeathP>
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<img src="https://static.igem.org/mediawiki/2012/b/b6/Groningen2012_AD_20120802_BigCog.png" width="250" height="250">
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<img src="https://static.igem.org/mediawiki/2012/c/c2/Groningen2012_RR_20120909_rotting.png" width="150" height="150">
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<font color=#FF6700><b>The Food Warden</b></font> is a system for detecting food spoilage. When a package of meat is saved after opening, a <font color=#FF6700><b>closed sticker</b></font> containing <i>Bacillus subtilis</i> spores is activated by breaking the middle compartment (similar to activating a glow-in-the-dark stick), whereby Alanine and water are mixed with the spores to cause <font color=#FF6700><b>germination</b></font>. When the germinated microbes come in contact with <font color=#FF6700><b>volatiles from rotting meat</b></font>, a pathway is <font color=#FF6700><b>activated</b></font> which results to the <font color=#FF6700><b>production of a pigment</b></font>. When this happens, the consumer knows that he has to throw away the food.<br> <br>
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Every year, one third of global food production -1.3 billion tons of food- is thrown away, partially due to the “best before” dating system. <font color=#FF6700><b>iGEM Groningen 2012</b></font> seeks to provide an alternative method of assessing edibility: the <font color=#FF6700><b>Food Warden</b></font>. It uses an <font color=#FF6700><b>engineered strain </b></font> of <i>Bacillus subtilis</i> to detect and report volatiles in spoiling meat. The introduced <font color=#FF6700><b>genetic construct</b></font> uses a promoter to trigger a pigment coding gene. This promoter, <font color=#FF6700><b>identified by microarray analysis</b></font>, is significantly up-regulated in the presence of <font color=#FF6700><b>volatiles from spoiling meat</b></font>. The activity of the <font color=#FF6700><b>promoter</b></font> regulates the expression of the <font color=#FF6700><b>pigment reporter</b></font> and will be visible to the naked eye. For safe usage of the system, spores of our engineered strain are placed into one half of a semi-permeable <font color=#FF6700><b>capsule</b></font>, the second containing a calibrated amount of nutrients. Breaking the barrier between the two compartments allows <font color=#FF6700><b>germination and growth</b></font>, thereby activating the <font color=#FF6700><b>spoiling meat sensor</b></font>.<br><br>
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</p><p class = "centered">Discover the different states of the Food Warden system below!</p>
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Latest revision as of 18:23, 23 September 2012






Abstract

sticker


germination


volatiles


identification


pigment


construct


kill switch





Every year, one third of global food production -1.3 billion tons of food- is thrown away, partially due to the “best before” dating system. iGEM Groningen 2012 seeks to provide an alternative method of assessing edibility: the Food Warden. It uses an engineered strain of Bacillus subtilis to detect and report volatiles in spoiling meat. The introduced genetic construct uses a promoter to trigger a pigment coding gene. This promoter, identified by microarray analysis, is significantly up-regulated in the presence of volatiles from spoiling meat. The activity of the promoter regulates the expression of the pigment reporter and will be visible to the naked eye. For safe usage of the system, spores of our engineered strain are placed into one half of a semi-permeable capsule, the second containing a calibrated amount of nutrients. Breaking the barrier between the two compartments allows germination and growth, thereby activating the spoiling meat sensor.


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