Team:Groningen/Notebook/Wetwork 27June2012

From 2012.igem.org

(Difference between revisions)
 
(14 intermediate revisions not shown)
Line 1: Line 1:
{{HeaderGroningen2012}}
{{HeaderGroningen2012}}
 +
<html>
 +
<body>
 +
<div style="position:absolute; right: 100px; top: 530px;">
-
Tom
 
-
Plasmid isolation of LuxR (BBa_R0062), LuxI (BBa_J37034) and backbone (BBa_090403) biobricks.
 
-
Plasmid concentrations: LuxR (1) = 99.3 ng/ul, LuxR (2) = 164.3 ng/ul, LuxI (1) = 69.9 ng/ul , LuxI (2) = 130.6 ng/ul, backbone = 63.0 ng/ul.
+
</div>
 +
</body>
 +
</html>
 +
<html>
 +
<head>
 +
<style type="text/css">
 +
p.margin{
 +
font-size:12pt;
 +
line-height:14pt;
 +
color:white;
 +
margin-top:0px;
 +
margin-bottom:20px;
 +
margin-left:150px;
 +
margin-right:250px;
 +
}
 +
</style>
 +
</head>
-
'''MicroArray experiment: preparations'''
+
<body>
 +
<p class="margin"><br><br><br><br>
 +
Tom <br> <br>
-
Arjan/Renske
+
Plasmid isolation of LuxR (BBa_R0062), LuxI (BBa_J37034) and backbone (BBa_090403) biobricks. <br> <br>
-
Made setup for the micro-array experiment:
+
Plasmid concentrations: LuxR (1) = 99.3 ng/ul, LuxR (2) = 164.3 ng/ul, LuxI (1) = 69.9 ng/ul , LuxI (2) = 130.6 ng/ul, backbone = 63.0 ng/ul. <br><br>
-
37 degrees room. Flask with 50 mL culture of Bacillus subtilis sp. 168, connected with tubes to 100 mL flask with rotting/fresh meat. Filters and peristaltic pump inbetween. Culture is stirred by a magnetic stirrer.
+
-
[[File:Groningen_RR_20120628_microarraysetup.jpg|thumb|400px|left|Micro-Array Setup]]
+
 
 +
Emeraldo/Nisa <br>
 +
<br>
 +
1.) Order SpeI and EcoRI FastDigest Fermentas<br>
 +
2.) Order Plamid High Pure purification kit Roche<br>
 +
3.) B. subtilis transformation with BBa_K090403 <br>
 +
4.) Digestion of pigment genes from their backbones with EcoRI and SpeI. Result: the pigments genes are in correct length. <br>
 +
5.) Digestion of BBa_K090403. Result: incorrect length.<br><br>
 +
 
 +
 
 +
MicroArray experiment: preparations <br><br>
 +
 
 +
Arjan/Renske <br><br>
 +
 
 +
Made setup for the micro-array experiment: <br>
 +
37 degrees room. Flask with 50 mL culture <br>
 +
of Bacillus subtilis sp. 168,<br>
 +
connected with tubes to 100 mL flask <br>
 +
with rotting/fresh meat. <br>
 +
Filters and peristaltic pump inbetween. <br>
 +
Culture is stirred by a magnetic stirrer.<br><br>
 +
<img src="https://static.igem.org/mediawiki/2012/9/9e/Groningen2012_RR_MicArraysetup.png" width="300" height="300">
 +
<br>
 +
<A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A>
 +
</p><br><br>
 +
</body>
 +
</html>
 +
 
 +
{{Template:SponsorsGroningen2012}}

Latest revision as of 16:52, 25 September 2012








Tom

Plasmid isolation of LuxR (BBa_R0062), LuxI (BBa_J37034) and backbone (BBa_090403) biobricks.

Plasmid concentrations: LuxR (1) = 99.3 ng/ul, LuxR (2) = 164.3 ng/ul, LuxI (1) = 69.9 ng/ul , LuxI (2) = 130.6 ng/ul, backbone = 63.0 ng/ul.

Emeraldo/Nisa

1.) Order SpeI and EcoRI FastDigest Fermentas
2.) Order Plamid High Pure purification kit Roche
3.) B. subtilis transformation with BBa_K090403
4.) Digestion of pigment genes from their backbones with EcoRI and SpeI. Result: the pigments genes are in correct length.
5.) Digestion of BBa_K090403. Result: incorrect length.

MicroArray experiment: preparations

Arjan/Renske

Made setup for the micro-array experiment:
37 degrees room. Flask with 50 mL culture
of Bacillus subtilis sp. 168,
connected with tubes to 100 mL flask
with rotting/fresh meat.
Filters and peristaltic pump inbetween.
Culture is stirred by a magnetic stirrer.


Back to notebook