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Team:Groningen/Notebook/Wetwork 22August2012
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| - | Cut AmilGFP, AmilCP, BBa_K274110, and pSac-CM+terminator (colony 3,6, and 10) with EcoRI and PstI, purified them from gel, inserting all the cut reporters inside the cut pSac-CM+terminator with ligation reaction, E. coli DH5alpha transformation with the ligated product. | + | Cut AmilGFP, AmilCP, BBa_K274110, and pSac-CM+terminator (colony 3,6, and 10) with EcoRI and PstI, purified them from gel, inserting all the cut reporters inside the cut pSac-CM+terminator (only psac from colony 3) with ligation reaction, E. coli DH5alpha transformation with the ligated product. |
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Revision as of 15:16, 25 September 2012
Yonathan and Elbrich
Digestion for preparation of the ligation of pFNR-violacein in pSac-Cm and pLacI-violacein in pSac-Cm.
Violacein had not the right size, so ligation of the construct was not possible.
Emeraldo
Cut AmilGFP, AmilCP, BBa_K274110, and pSac-CM+terminator (colony 3,6, and 10) with EcoRI and PstI, purified them from gel, inserting all the cut reporters inside the cut pSac-CM+terminator (only psac from colony 3) with ligation reaction, E. coli DH5alpha transformation with the ligated product.
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